Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato

Savidor A, Chalupowicz L, Teper D, Gartemann K-H, Eichenlaub R, Manulis-Sasson S, Barash I, Sessa G (2014)
Molecular Plant-Microbe Interactions 27(10): 1035-1047.

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The plant pathogen Clavibacter michiganensis subsp. michiganensis is a gram-positive bacterium responsible for wilt and canker disease of tomato. Although disease development is well characterized and diagnosed, molecular mechanisms of C. michiganensis subsp. michiganensis virulence are poorly understood. Here, we identified and characterized two C. michiganensis subsp. michiganensis transcriptional regulators, Vatr1 and Vatr2, that are involved in pathogenicity of C. michiganensis subsp. michiganensis. Vatr1 and Vatr2 belong to TetR and MocR families of transcriptional regulators, respectively. Mutations in their corresponding genes caused attenuated virulence, with the Delta vatr2 mutant showing a more dramatic effect than Delta vatr1. Although both mutants grew well in vitro and reached a high titer in planta, they caused reduced wilting and canker development in infected plants compared with the wild-type bacterium. They also led to a reduced expression of the ethylene-synthesizing tomato enzyme ACC-oxidase compared with wild-type C. michiganensis subsp. michiganensis and to reduced ethylene production in the plant. Transcriptomic analysis of wild-type C. michiganensis subsp. michiganensis and the two mutants under infectionmimicking conditions revealed that Vatr1 and Vatr2 regulate expression of virulence factors, membrane and secreted proteins, and signal-transducing proteins. A 70% overlap between the sets of genes positively regulated by Vatr1 and Vatr2 suggests that these transcriptional regulators are on the same molecular pathway responsible for C. michiganensis subsp. michiganensis virulence.
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Savidor A, Chalupowicz L, Teper D, et al. Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions. 2014;27(10):1035-1047.
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K. - H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., et al. (2014). Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions, 27(10), 1035-1047. doi:10.1094/MPMI-02-14-0061-R
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K. - H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., and Sessa, G. (2014). Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions 27, 1035-1047.
Savidor, A., et al., 2014. Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions, 27(10), p 1035-1047.
A. Savidor, et al., “Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato”, Molecular Plant-Microbe Interactions, vol. 27, 2014, pp. 1035-1047.
Savidor, A., Chalupowicz, L., Teper, D., Gartemann, K.-H., Eichenlaub, R., Manulis-Sasson, S., Barash, I., Sessa, G.: Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato. Molecular Plant-Microbe Interactions. 27, 1035-1047 (2014).
Savidor, Alon, Chalupowicz, Laura, Teper, Doron, Gartemann, Karl-Heinz, Eichenlaub, Rudolf, Manulis-Sasson, Shulamit, Barash, Isaac, and Sessa, Guido. “Clavibacter michiganensis subsp michiganensis Vatr1 and Vatr2 Transcriptional Regulators Are Required for Virulence in Tomato”. Molecular Plant-Microbe Interactions 27.10 (2014): 1035-1047.
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4 Citations in Europe PMC

Data provided by Europe PubMed Central.

Differential contribution of Clavibacter michiganensis ssp. michiganensis virulence factors to systemic and local infection in tomato.
Chalupowicz L, Barash I, Reuven M, Dror O, Sharabani G, Gartemann KH, Eichenlaub R, Sessa G, Manulis-Sasson S., Mol Plant Pathol 18(3), 2017
PMID: 26992141
Genomic Analysis of Clavibacter michiganensis Reveals Insight Into Virulence Strategies and Genetic Diversity of a Gram-Positive Bacterial Pathogen.
Thapa SP, Pattathil S, Hahn MG, Jacques MA, Gilbertson RL, Coaker G., Mol Plant Microbe Interact 30(10), 2017
PMID: 28677494
A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants.
Hiery E, Poetsch A, Moosbauer T, Amin B, Hofmann J, Burkovski A., Proteomes 3(4), 2015
PMID: 28248277

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