The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length

Tamhane T, Arampatzidou M, Gerganova V, Tacke M, Illukkumbura R, Dauth S, Schaschke N, Peters C, Reinheckel T, Brix K (2014)
Biological Chemistry 395(10): 1201-1219.

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Abstract
Cysteine cathepsins are expressed in most tissues, including the gastrointestinal tract. We demonstrated an involvement of mouse intestinal cathepsin B in extracellular matrix remodeling for regeneration from trauma. The present study aimed at elucidating roles of cysteine cathepsins in the non-traumatized gastrointestinal tract of mice. Thus we investigated expression and localization patterns of cathepsin B and its closest relative, cathepsin X, along the length of the gastrointestinal tract, and determined the effects of their absence. Cathepsin B showed the highest protein levels in the anterior segments of the gastrointestinal tract, whereas the highest activity was observed in the jejunum, as revealed by cathepsin B-specific activity-based probe labeling. Cathepsin X was most abundant in the jejunum and protein levels were elevated in duodenum and colon of Ctsb(-/-) mice. The segmental pattern of cathepsin expression was reflected by a compartmentalized distribution of junction proteins and basal lamina constituents, changes in tissue architecture and altered activities of the brush border enzyme aminopeptidase N. In conclusion, we observed different compensatory effects and activity levels of cysteine peptidases along the length of the small and large intestines in a segment-specific manner suggesting specific in situ functions of these enzymes in particular parts of the gastrointestinal tract.
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Tamhane T, Arampatzidou M, Gerganova V, et al. The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length. Biological Chemistry. 2014;395(10):1201-1219.
Tamhane, T., Arampatzidou, M., Gerganova, V., Tacke, M., Illukkumbura, R., Dauth, S., Schaschke, N., et al. (2014). The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length. Biological Chemistry, 395(10), 1201-1219.
Tamhane, T., Arampatzidou, M., Gerganova, V., Tacke, M., Illukkumbura, R., Dauth, S., Schaschke, N., Peters, C., Reinheckel, T., and Brix, K. (2014). The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length. Biological Chemistry 395, 1201-1219.
Tamhane, T., et al., 2014. The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length. Biological Chemistry, 395(10), p 1201-1219.
T. Tamhane, et al., “The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length”, Biological Chemistry, vol. 395, 2014, pp. 1201-1219.
Tamhane, T., Arampatzidou, M., Gerganova, V., Tacke, M., Illukkumbura, R., Dauth, S., Schaschke, N., Peters, C., Reinheckel, T., Brix, K.: The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length. Biological Chemistry. 395, 1201-1219 (2014).
Tamhane, Tripti, Arampatzidou, Maria, Gerganova, Veneta, Tacke, Marlene, Illukkumbura, Rukshala, Dauth, Stephanie, Schaschke, Norbert, Peters, Christoph, Reinheckel, Thomas, and Brix, Klaudia. “The activity and localization patterns of cathepsins B and X in cells of the mouse gastrointestinal tract differ along its length”. Biological Chemistry 395.10 (2014): 1201-1219.
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Construction of a plasmid coding for green fluorescent protein tagged cathepsin L and data on expression in colorectal carcinoma cells.
Tamhane T, Wolters BK, Illukkumbura R, Maelandsmo GM, Haugen MH, Brix K., Data Brief 5(), 2015
PMID: 26594658
Design of a highly selective quenched activity-based probe and its application in dual color imaging studies of cathepsin S activity localization.
Oresic Bender K, Ofori L, van der Linden WA, Mock ED, Datta GK, Chowdhury S, Li H, Segal E, Sanchez Lopez M, Ellman JA, Figdor CG, Bogyo M, Verdoes M., J. Am. Chem. Soc. 137(14), 2015
PMID: 25785540
Proteolysis mediated by cysteine cathepsins and legumain-recent advances and cell biological challenges.
Brix K, McInnes J, Al-Hashimi A, Rehders M, Tamhane T, Haugen MH., Protoplasma 252(3), 2015
PMID: 25398648

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