Sortagging: a robust and efficient chemoenzymatic ligation strategy

Ritzefeld M (2014)
Chemistry (Weinheim an der Bergstrasse, Germany) 20(28): 8516-8529.

Journal Article | Published | English

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Abstract
Bioorthogonal, chemoselective ligation methods are an essential part of the tools utilized to investigate biochemical pathways. Specifically enzymatic approaches are valuable methods in this context due to the inherent specificity of the deployed enzymes and the mild conditions of the modification reactions. One of the most common strategies is based on the transpeptidation catalyzed by sortase A derived from Staphylococcus aureus. The procedure is well established and a wide variety of applications have been published to date. Here, implementations of sortase A, which range from protein labeling using fluorescence dyes and the preparation of cyclic proteins to the modification of entire cells, are summarized. Furthermore, there is a focus on the optimization approaches established to solve the drawbacks of sortase-mediated transpeptidation.
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Ritzefeld M. Sortagging: a robust and efficient chemoenzymatic ligation strategy. Chemistry (Weinheim an der Bergstrasse, Germany). 2014;20(28):8516-8529.
Ritzefeld, M. (2014). Sortagging: a robust and efficient chemoenzymatic ligation strategy. Chemistry (Weinheim an der Bergstrasse, Germany), 20(28), 8516-8529.
Ritzefeld, M. (2014). Sortagging: a robust and efficient chemoenzymatic ligation strategy. Chemistry (Weinheim an der Bergstrasse, Germany) 20, 8516-8529.
Ritzefeld, M., 2014. Sortagging: a robust and efficient chemoenzymatic ligation strategy. Chemistry (Weinheim an der Bergstrasse, Germany), 20(28), p 8516-8529.
M. Ritzefeld, “Sortagging: a robust and efficient chemoenzymatic ligation strategy”, Chemistry (Weinheim an der Bergstrasse, Germany), vol. 20, 2014, pp. 8516-8529.
Ritzefeld, M.: Sortagging: a robust and efficient chemoenzymatic ligation strategy. Chemistry (Weinheim an der Bergstrasse, Germany). 20, 8516-8529 (2014).
Ritzefeld, Markus. “Sortagging: a robust and efficient chemoenzymatic ligation strategy”. Chemistry (Weinheim an der Bergstrasse, Germany) 20.28 (2014): 8516-8529.
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1 Citation in Europe PMC

Data provided by Europe PubMed Central.

Covalent Modification of Synthetic Hydrogels with Bioactive Proteins via Sortase-Mediated Ligation.
Cambria E, Renggli K, Ahrens CC, Cook CD, Kroll C, Krueger AT, Imperiali B, Griffith LG., Biomacromolecules 16(8), 2015
PMID: 26098148

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