Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy
Mönkemöller V, Schüttpelz M, McCourt P, Sorensen K, Smedsrod B, Huser T (2014)
Physical Chemistry Chemical Physics 16(24): 12576-12581.
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Mönkemöller, ViolaUniBi
;
Schüttpelz, MarkUniBi
;
McCourt, Peter
;
Sorensen, Karen
;
Smedsrod, Bard
;
Huser, ThomasUniBi

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Abstract
Liver sinusoidal endothelial cells (LSEC) are an important class of endothelial cells facilitating the translocation of lipoproteins and small molecules between the liver and blood. A number of clinical conditions, especially metabolic and aging-related disorders, are implicated by improper function of LSECs. Despite their importance, research into these cells is limited because the primary ultrastructures involved in their function are transcellular pores, called fenestrations, with diameters in a size range between 50-200 nm, i.e. well below the optical diffraction limit. Here, we show that we are able to resolve fenestrations with a spatial resolution of similar to 20 nm by direct stochastic optical reconstruction microscopy (dSTORM). The cellular plasma membrane was labeled at high fluorophore density with CellMask Deep Red and imaged using a reducing buffer system. We compare the higher degree of structural detail that dSTORM provides to results obtained by 3D structured illumination microscopy (3D-SIM). Our results open up a path to image these physiologically important cells in vitro using highly resolving localization microscopy techniques that could be implemented on non-specialized fluorescence microscopes, enabling their investigation in most biomedical laboratories without the need for electron microscopy.
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Mönkemöller V, Schüttpelz M, McCourt P, Sorensen K, Smedsrod B, Huser T. Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics. 2014;16(24):12576-12581.
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., & Huser, T. (2014). Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics, 16(24), 12576-12581. doi:10.1039/c4cp01574f
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., and Huser, T. (2014). Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics 16, 12576-12581.
Mönkemöller, V., et al., 2014. Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics, 16(24), p 12576-12581.
V. Mönkemöller, et al., “Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy”, Physical Chemistry Chemical Physics, vol. 16, 2014, pp. 12576-12581.
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., Huser, T.: Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics. 16, 12576-12581 (2014).
Mönkemöller, Viola, Schüttpelz, Mark, McCourt, Peter, Sorensen, Karen, Smedsrod, Bard, and Huser, Thomas. “Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy”. Physical Chemistry Chemical Physics 16.24 (2014): 12576-12581.
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5 Citations in Europe PMC
Data provided by Europe PubMed Central.
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PMID: 26996201
Müller M, Mönkemöller V, Hennig S, Hübner W, Huser T., Nat Commun 7(), 2016
PMID: 26996201
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PMID: 27958271
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PMID: 27958271
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PMID: 25767958
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PMID: 25767958
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PMID: 26549018
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PMID: 26549018
A First-Time-In-Human Phase I Clinical Trial of Bispecific Antibody-Targeted, Paclitaxel-Packaged Bacterial Minicells.
Solomon BJ, Desai J, Rosenthal M, McArthur GA, Pattison ST, Pattison SL, MacDiarmid J, Brahmbhatt H, Scott AM., PLoS One 10(12), 2015
PMID: 26659127
Solomon BJ, Desai J, Rosenthal M, McArthur GA, Pattison ST, Pattison SL, MacDiarmid J, Brahmbhatt H, Scott AM., PLoS One 10(12), 2015
PMID: 26659127
29 References
Data provided by Europe PubMed Central.
Live-cell super-resolution imaging with trimethoprim conjugates.
Wombacher R, Heidbreder M, van de Linde S, Sheetz MP, Heilemann M, Cornish VW, Sauer M., Nat. Methods 7(9), 2010
PMID: 20693998
Wombacher R, Heidbreder M, van de Linde S, Sheetz MP, Heilemann M, Cornish VW, Sauer M., Nat. Methods 7(9), 2010
PMID: 20693998
Yahiatene, Chem. Phys. Lett. 587(), 2013
Fast, background-free, 3D super-resolution optical fluctuation imaging (SOFI).
Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J., Proc. Natl. Acad. Sci. U.S.A. 106(52), 2009
PMID: 20018714
Dertinger T, Colyer R, Iyer G, Weiss S, Enderlein J., Proc. Natl. Acad. Sci. U.S.A. 106(52), 2009
PMID: 20018714
A blueprint for cost-efficient localization microscopy.
Holm T, Klein T, Loschberger A, Klamp T, Wiebusch G, van de Linde S, Sauer M., Chemphyschem 15(4), 2014
PMID: 24227751
Holm T, Klein T, Loschberger A, Klamp T, Wiebusch G, van de Linde S, Sauer M., Chemphyschem 15(4), 2014
PMID: 24227751
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