Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy

Mönkemöller V, Schüttpelz M, McCourt P, Sorensen K, Smedsrod B, Huser T (2014)
Physical Chemistry Chemical Physics 16(24): 12576-12581.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Liver sinusoidal endothelial cells (LSEC) are an important class of endothelial cells facilitating the translocation of lipoproteins and small molecules between the liver and blood. A number of clinical conditions, especially metabolic and aging-related disorders, are implicated by improper function of LSECs. Despite their importance, research into these cells is limited because the primary ultrastructures involved in their function are transcellular pores, called fenestrations, with diameters in a size range between 50-200 nm, i.e. well below the optical diffraction limit. Here, we show that we are able to resolve fenestrations with a spatial resolution of similar to 20 nm by direct stochastic optical reconstruction microscopy (dSTORM). The cellular plasma membrane was labeled at high fluorophore density with CellMask Deep Red and imaged using a reducing buffer system. We compare the higher degree of structural detail that dSTORM provides to results obtained by 3D structured illumination microscopy (3D-SIM). Our results open up a path to image these physiologically important cells in vitro using highly resolving localization microscopy techniques that could be implemented on non-specialized fluorescence microscopes, enabling their investigation in most biomedical laboratories without the need for electron microscopy.
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Zeitschriftentitel
Physical Chemistry Chemical Physics
Band
16
Zeitschriftennummer
24
Seite
12576-12581
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Mönkemöller V, Schüttpelz M, McCourt P, Sorensen K, Smedsrod B, Huser T. Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics. 2014;16(24):12576-12581.
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., & Huser, T. (2014). Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics, 16(24), 12576-12581. doi:10.1039/c4cp01574f
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., and Huser, T. (2014). Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics 16, 12576-12581.
Mönkemöller, V., et al., 2014. Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics, 16(24), p 12576-12581.
V. Mönkemöller, et al., “Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy”, Physical Chemistry Chemical Physics, vol. 16, 2014, pp. 12576-12581.
Mönkemöller, V., Schüttpelz, M., McCourt, P., Sorensen, K., Smedsrod, B., Huser, T.: Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy. Physical Chemistry Chemical Physics. 16, 12576-12581 (2014).
Mönkemöller, Viola, Schüttpelz, Mark, McCourt, Peter, Sorensen, Karen, Smedsrod, Bard, and Huser, Thomas. “Imaging fenestrations in liver sinusoidal endothelial cells by optical localization microscopy”. Physical Chemistry Chemical Physics 16.24 (2014): 12576-12581.

5 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

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