Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation

Schürmann M, Wolff A, Widera D, Hauser S, Heimann P, Hütten A, Kaltschmidt C, Kaltschmidt B (2014)
Stem Cell Research 13(1): 98-110.

Download
OA
Journal Article | Published | English
Abstract
Abstract Osteogenic differentiation of various adult stem cell populations such as neural crest-derived stem cells is of great interest in the context of bone regeneration. Ideally, exogenous differentiation should mimic an endogenous differentiation process, which is partly mediated by topological cues. To elucidate the osteoinductive potential of porous substrates with different pore diameters (30 nm, 100 nm), human neural crest-derived stem cells isolated from the inferior nasal turbinate were cultivated on the surface of nanoporous titanium covered membranes without additional chemical or biological osteoinductive cues. As controls, flat titanium without any topological features and osteogenic medium was used. Cultivation of human neural crest-derived stem cells on 30 nm pores resulted in osteogenic differentiation as demonstrated by alkaline phosphatase activity after seven days as well as by calcium deposition after 3 weeks of cultivation. In contrast, cultivation on flat titanium and on membranes equipped with 100 nm pores was not sufficient to induce osteogenic differentiation. Moreover, we demonstrate an increase of osteogenic transcripts including Osterix, Osteocalcin and up-regulation of Integrin β1 and α2 in the 30 nm pore approach only. Thus, transplantation of stem cells pre-cultivated on nanostructured implants might improve the clinical outcome by support of the graft adherence and acceleration of the regeneration process.
Publishing Year
ISSN
PUB-ID

Cite this

Schürmann M, Wolff A, Widera D, et al. Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research. 2014;13(1):98-110.
Schürmann, M., Wolff, A., Widera, D., Hauser, S., Heimann, P., Hütten, A., Kaltschmidt, C., et al. (2014). Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research, 13(1), 98-110.
Schürmann, M., Wolff, A., Widera, D., Hauser, S., Heimann, P., Hütten, A., Kaltschmidt, C., and Kaltschmidt, B. (2014). Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research 13, 98-110.
Schürmann, M., et al., 2014. Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research, 13(1), p 98-110.
M. Schürmann, et al., “Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation”, Stem Cell Research, vol. 13, 2014, pp. 98-110.
Schürmann, M., Wolff, A., Widera, D., Hauser, S., Heimann, P., Hütten, A., Kaltschmidt, C., Kaltschmidt, B.: Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation. Stem Cell Research. 13, 98-110 (2014).
Schürmann, Matthias, Wolff, Annalena, Widera, Darius, Hauser, Stefan, Heimann, Peter, Hütten, Andreas, Kaltschmidt, Christian, and Kaltschmidt, Barbara. “Interaction of adult human neural crest-derived stem cells with nanoporous titanium surface is sufficient to induce their osteogenic differentiation”. Stem Cell Research 13.1 (2014): 98-110.
Main File(s)
Access Level
OA Open Access
Last Uploaded
2014-06-10 14:34:18

This data publication is cited in the following publications:
This publication cites the following data publications:

1 Citation in Europe PMC

Data provided by Europe PubMed Central.

A fibrin matrix promotes the differentiation of EMSCs isolated from nasal respiratory mucosa to myelinating phenotypical Schwann-like cells.
Chen Q, Zhang Z, Liu J, He Q, Zhou Y, Shao G, Sun X, Cao X, Gong A, Jiang P., Mol. Cells 38(3), 2015
PMID: 25666351

39 References

Data provided by Europe PubMed Central.

The lamina propria of adult human oral mucosa harbors a novel stem cell population.
Marynka-Kalmani K, Treves S, Yafee M, Rachima H, Gafni Y, Cohen MA, Pitaru S., Stem Cells 28(5), 2010
PMID: 20474080
Prolonged cultivation of hippocampal neural precursor cells shifts their differentiation potential and selects for aneuploid cells.
Nguyen TD, Widera D, Greiner J, Muller J, Martin I, Slotta C, Hauser S, Kaltschmidt C, Kaltschmidt B., Biol. Chem. 394(12), 2013
PMID: 24084358
The transcriptional activity of osterix requires the recruitment of Sp1 to the osteocalcin proximal promoter.
Niger C, Lima F, Yoo DJ, Gupta RR, Buo AM, Hebert C, Stains JP., Bone 49(4), 2011
PMID: 21820092
Stem cell fate dictated solely by altered nanotube dimension.
Oh S, Brammer KS, Li YS, Teng D, Engler AJ, Chien S, Jin S., Proc. Natl. Acad. Sci. U.S.A. 106(7), 2009
PMID: 19179282
Substrate affects the initial attachment and subsequent behavior of human osteoblastic cells (Saos-2).
Okumura A, Goto M, Goto T, Yoshinari M, Masuko S, Katsuki T, Tanaka T., Biomaterials 22(16), 2001
PMID: 11456066
Integrin alpha2beta1 plays a critical role in osteoblast response to micron-scale surface structure and surface energy of titanium substrates.
Olivares-Navarrete R, Raz P, Zhao G, Chen J, Wieland M, Cochran DL, Chaudhri RA, Ornoy A, Boyan BD, Schwartz Z., Proc. Natl. Acad. Sci. U.S.A. 105(41), 2008
PMID: 18843104
Nanosize and vitality: TiO2 nanotube diameter directs cell fate.
Park J, Bauer S, von der Mark K, Schmuki P., Nano Lett. 7(6), 2007
PMID: 17503870
Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.
Salasznyk RM, Klees RF, Boskey A, Plopper GE., J. Cell. Biochem. 100(2), 2007
PMID: 16927379
Titanium nanofeaturing for enhanced bioactivity of implanted orthopedic and dental devices. [Research Support, Non-U.S. Gov't Review]
Sjostrom, Nanomedicine (London) 8(1), 2013
Isolation of distinct progenitor stem cell populations from dental pulp
Waddington, Cells Tissues Organs 189(1–4), 2009
Integrin beta1 silencing in osteoblasts alters substrate-dependent responses to 1,25-dihydroxy vitamin D3.
Wang L, Zhao G, Olivares-Navarrete R, Bell BF, Wieland M, Cochran DL, Schwartz Z, Boyan BD., Biomaterials 27(20), 2006
PMID: 16569430
Effects of titanium surface topography on bone integration: a systematic review.
Wennerberg A, Albrektsson T., Clin Oral Implants Res 20 Suppl 4(), 2009
PMID: 19663964
Highly efficient neural differentiation of human somatic stem cells, isolated by minimally invasive periodontal surgery.
Widera D, Grimm WD, Moebius JM, Mikenberg I, Piechaczek C, Gassmann G, Wolff NA, Thevenod F, Kaltschmidt C, Kaltschmidt B., Stem Cells Dev. 16(3), 2007
PMID: 17610375
Adult palatum as a novel source of neural crest-related stem cells.
Widera D, Zander C, Heidbreder M, Kasperek Y, Noll T, Seitz O, Saldamli B, Sudhoff H, Sader R, Kaltschmidt C, Kaltschmidt B., Stem Cells 27(8), 2009
PMID: 19544446

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 24858494
PubMed | Europe PMC

Search this title in

Google Scholar