Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models

Kolditz F, Krausze J, Heinz DW, Niemann H, Müller-Goymann CC (2014)
European journal of pharmaceutics and biopharmaceutics 86(2: Theme Issue: Dermal and Transdermal Drug Delivery): 277-283.

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Abstract
A constitutively dimeric truncated variant of internalin B (InlB321-CD), acting as stimulator of the receptor tyrosine kinase MET, was tested for dermal wound-healing potential. Due to a lack of the endogenous MET agonist HGF/SF in chronic wounds, HGF/SF substitution by an InlB321-CD-loaded hydrogel might be beneficial in chronic wound therapy. In this study, InlB321-CD in solution and incorporated in a hydrogel was tested for mitogenic effects on immortalized human dermal keratinocytes (HaCaT) with an MTT assay. Cell migration was investigated with a scratch assay on primary keratinocytes (PHK) and on HaCaT. For the latter, scratching needed to be mitomycin C-controlled. InlB321-CD effects on a model of human skin were analyzed histologically with respect to viability. InlB321-CD led to dose-dependent proliferative effects on HaCaT cells whereas the equimolar dose of monomeric InlB321 did not. Upon hydrogel incorporation of InlB321-CD its mitogenic activity for HaCaT cells was maintained thus confirming the hydrogel as a promising drug delivery system. Motogenic effects were shown on both HaCaT and PHK cells. InlB321-CD neither possesses cytotoxic effects on the viability of a human skin model nor alters its organotypic cell morphology.
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Kolditz F, Krausze J, Heinz DW, Niemann H, Müller-Goymann CC. Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models. European journal of pharmaceutics and biopharmaceutics. 2014;86(2: Theme Issue: Dermal and Transdermal Drug Delivery):277-283.
Kolditz, F., Krausze, J., Heinz, D. W., Niemann, H., & Müller-Goymann, C. C. (2014). Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models. European journal of pharmaceutics and biopharmaceutics, 86(2: Theme Issue: Dermal and Transdermal Drug Delivery), 277-283. doi:10.1016/j.ejpb.2013.10.003
Kolditz, F., Krausze, J., Heinz, D. W., Niemann, H., and Müller-Goymann, C. C. (2014). Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models. European journal of pharmaceutics and biopharmaceutics 86, 277-283.
Kolditz, F., et al., 2014. Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models. European journal of pharmaceutics and biopharmaceutics, 86(2: Theme Issue: Dermal and Transdermal Drug Delivery), p 277-283.
F. Kolditz, et al., “Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models”, European journal of pharmaceutics and biopharmaceutics, vol. 86, 2014, pp. 277-283.
Kolditz, F., Krausze, J., Heinz, D.W., Niemann, H., Müller-Goymann, C.C.: Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models. European journal of pharmaceutics and biopharmaceutics. 86, 277-283 (2014).
Kolditz, F., Krausze, J., Heinz, D. W., Niemann, Hartmut, and Müller-Goymann, C. C. “Wound healing potential of a dimeric InlB variant analyzed by in vitro experiments on re-epithelialization of human skin models”. European journal of pharmaceutics and biopharmaceutics 86.2: Theme Issue: Dermal and Transdermal Drug Delivery (2014): 277-283.
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