Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites

Zoerb C, Becker D, Hasler M, Muehling K, Gödde V, Niehaus K, Geilfus C-M (2013)
Frontiers in Plant Science 4.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ;
Abstract
Wheat is an important source of proteins and metabolites for human and animal nutrition. To assess the nutritional quality of wheat products, various protein and diverse metabolites have to be evaluated. The grain storage protein family of the alpha-gliadins are suggested to be the primary initiator of the inflammatory response to gluten in Celiac disease patients. With the technique of RNAi, the alpha-gliadin storage protein fraction in wheat grains was recently knocked down. From a patient's perspective, this is a desired approach, however, this study aims to evaluate whether such a down-regulation of these problematic alpha-gliadins also has unintended side-effects on other plant metabolites. Such uncontrolled and unknown arbitrary effects on any metabolite in plants designated for food production would surely represent an avoidable risk for the consumer. In general, alpha-gliadins are rich in sulfur, making their synthesis and content depended of the sulfur supply. For this reason, the influence of the application of increasing sulfur amounts on the metabolome of alpha-gliadin-deficient wheat was additionally investigated because it might be possible that e.g., considerable high/low amounts of S might increase or even induce such unintended effects that are not observable under moderate S nutrition. By silencing the alpha-gliadin genes, a recently developed wheat line that lacks the set of 75 corresponding alpha-gliadin proteins has become available. The plants were subsequently tested for RNAi-induced effects on metabolites that were not directly attributable to the specific effects of the RNAi-approach on the alpha-gliadin proteins. For this, GC-MS-based metabolite profiles were recorded. A comparison of wild type with gliadin-deficient plants cultivated in pot experiments revealed no differences in all 109 analyzed metabolites, regardless of the S-nutritional status. No unintended effects attributable to the RNAi-based specific genetic deletion of a storage protein fraction were observed.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Zoerb C, Becker D, Hasler M, et al. Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites. Frontiers in Plant Science. 2013;4.
Zoerb, C., Becker, D., Hasler, M., Muehling, K., Gödde, V., Niehaus, K., & Geilfus, C. - M. (2013). Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites. Frontiers in Plant Science, 4.
Zoerb, C., Becker, D., Hasler, M., Muehling, K., Gödde, V., Niehaus, K., and Geilfus, C. - M. (2013). Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites. Frontiers in Plant Science 4.
Zoerb, C., et al., 2013. Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites. Frontiers in Plant Science, 4.
C. Zoerb, et al., “Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites”, Frontiers in Plant Science, vol. 4, 2013.
Zoerb, C., Becker, D., Hasler, M., Muehling, K., Gödde, V., Niehaus, K., Geilfus, C.-M.: Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites. Frontiers in Plant Science. 4, (2013).
Zoerb, Christian, Becker, Dirk, Hasler, Mario, Muehling, Karlh., Gödde, Victoria, Niehaus, Karsten, and Geilfus, Christoph-Martin. “Silencing of the sulfur rich alpha-gliadin storage protein family in wheat grains (Triticum aestivum L.) causes no unintended side-effects on other metabolites”. Frontiers in Plant Science 4 (2013).
This data publication is cited in the following publications:
This publication cites the following data publications:

22 References

Data provided by Europe PubMed Central.

Protein composition and techno-functional properties of transgenic wheat with reduced α –gliadin content obtained by RNA interference
Becker D., Wieser H., Köhler P., Folck A., Mühling K., Zörb C.., 2012
Metabolite profiling for plant functional genomics.
Fiehn O, Kopka J, Dormann P, Altmann T, Trethewey RN, Willmitzer L., Nat. Biotechnol. 18(11), 2000
PMID: 11062433
Celiac disease: prevalence, diagnosis, pathogenesis and treatment.
Gujral N, Freeman HJ, Thomson AB., World J. Gastroenterol. 18(42), 2012
PMID: 23155333

Hartung J., Elpelt B.., 1999
Multiple contrast tests in the presence of heteroscedasticity.
Hasler M, Hothorn LA., Biom J 50(5), 2008
PMID: 18932141
A simple sequentially rejective multiple test procedure
Holm S.., 1979
Co-ordinated expression of amino acid metabolism in response to N and S deficiency during wheat grain filling.
Howarth JR, Parmar S, Jones J, Shepherd CE, Corol DI, Galster AM, Hawkins ND, Miller SJ, Baker JM, Verrier PJ, Ward JL, Beale MH, Barraclough PB, Hawkesford MJ., J. Exp. Bot. 59(13), 2008
PMID: 18791197
How does scientific risk assessment of GM crops fit within the wider risk analysis?
Johnson KL, Raybould AF, Hudson MD, Poppy GM., Trends Plant Sci. 12(1), 2007
PMID: 17161972
GMD@CSB.DB: the Golm Metabolome Database.
Kopka J, Schauer N, Krueger S, Birkemeyer C, Usadel B, Bergmuller E, Dormann P, Weckwerth W, Gibon Y, Stitt M, Willmitzer L, Fernie AR, Steinhauser D., Bioinformatics 21(8), 2005
PMID: 15613389
A uniform decimal code for growth stages of crops and weeds.
Lancashire PD, Bleiholder H, Boom Tvanden, Langeluddeke P, Stauss R, Weber E, Witzenberger A., Ann. Appl. Biol. 119(3), 1991
PMID: IND92024777

AUTHOR UNKNOWN, 2012
Sulfur metabolism in plants: are trees different?
Rennenberg H, Herschbach C, Haberer K, Kopriva S., Plant Biol (Stuttg) 9(5), 2007
PMID: 17853362
Analysis of trials with complex treatment structure using multiple contrast tests
Schaarschmidt F., Vaas L.., 2009
Sulphur in crop production--invited paper.
Scherer HW., European journal of agronomy : the journal of the European Society for Agronomy. 14(2), 2001
PMID: IND22906946
Structural basis for gluten intolerance in celiac sprue.
Shan L, Molberg O, Parrot I, Hausch F, Filiz F, Gray GM, Sollid LM, Khosla C., Science 297(5590), 2002
PMID: 12351792
Wheat.
Shewry PR., J. Exp. Bot. 60(6), 2009
PMID: 19386614
The precipitating factor in coeliac disease.
Wieser H., Baillieres Clin. Gastroenterol. 9(2), 1995
PMID: 7549024
Metabolomic responses in grain, ear and straw of winter wheat under increasing sulphur treatment
Zörb C., Mühling K., Hasler M., Gödde V., Niehaus K., Becker D.., 2013
Metabolite profiling of wheat flag leaf and grains during grain filling phase as affected by S fertilization
Zörb C., Steinfurth D., Gödde V., Niehaus K., Mühling K.., 2012

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 24062763
PubMed | Europe PMC

Search this title in

Google Scholar