Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation

Myronovskyi M, Tokovenko B, Brötz E, Rückert C, Kalinowski J, Luzhetskyy A (2014)
Applied microbiology and biotechnology 98(2): 795-806.

Download
Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Autor
; ; ; ; ;
Abstract / Bemerkung
Streptomyces albus J1074 is a derivative of the S. albus G1 strain defective in SalG1 restriction-modification system. Genome sequencing of S. albus J1074 revealed that the size of its chromosome is 6.8 Mb with unusually short terminal arms of only 0.3 and 0.4 Mb. Here we present our attempts to evaluate the dispensability of subtelomeric regions of the S. albus J1074 chromosome. A number of large site-directed genomic deletions led to circularization of the S. albus J1074 chromosome and to the overall genome reduction by 307 kb. Two spontaneous mutants with an activated carotenoid cluster were obtained. Genome sequencing and transcriptome analysis indicated that phenotypes of these mutants resulted from the right terminal 0.42 Mb chromosomal region deletion, followed by the carotenoid cluster amplification. Our results indicate that the right terminal 0.42 Mb fragment is dispensable under laboratory conditions. In contrast, the left terminal arm of the S. albus J1074 chromosome contains essential genes and only 42 kb terminal region is proved to be dispensable. We identified overexpressed carotenoid compounds and determined fitness costs of the large genomic rearrangements.
Erscheinungsjahr
Zeitschriftentitel
Applied microbiology and biotechnology
Band
98
Zeitschriftennummer
2
Seite
795-806
ISSN
eISSN
PUB-ID

Zitieren

Myronovskyi M, Tokovenko B, Brötz E, Rückert C, Kalinowski J, Luzhetskyy A. Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology. 2014;98(2):795-806.
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., & Luzhetskyy, A. (2014). Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology, 98(2), 795-806. doi:10.1007/s00253-013-5440-6
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., and Luzhetskyy, A. (2014). Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology 98, 795-806.
Myronovskyi, M., et al., 2014. Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology, 98(2), p 795-806.
M. Myronovskyi, et al., “Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation”, Applied microbiology and biotechnology, vol. 98, 2014, pp. 795-806.
Myronovskyi, M., Tokovenko, B., Brötz, E., Rückert, C., Kalinowski, J., Luzhetskyy, A.: Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation. Applied microbiology and biotechnology. 98, 795-806 (2014).
Myronovskyi, Maksym, Tokovenko, Bogdan, Brötz, Elke, Rückert, Christian, Kalinowski, Jörn, and Luzhetskyy, Andriy. “Genome rearrangements of Streptomyces albus J1074 lead to the carotenoid gene cluster activation”. Applied microbiology and biotechnology 98.2 (2014): 795-806.

6 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Identification of butenolide regulatory system controlling secondary metabolism in Streptomyces albus J1074.
Ahmed Y, Rebets Y, Tokovenko B, Brötz E, Luzhetskyy A., Sci Rep 7(1), 2017
PMID: 28852167
Novel Bioactive Paulomycin Derivatives Produced by Streptomyces albus J1074.
Hoz JF, Méndez C, Salas JA, Olano C., Molecules 22(10), 2017
PMID: 29057800
Identification and activation of novel biosynthetic gene clusters by genome mining in the kirromycin producer Streptomyces collinus Tü 365.
Iftime D, Kulik A, Härtner T, Rohrer S, Niedermeyer TH, Stegmann E, Weber T, Wohlleben W., J Ind Microbiol Biotechnol 43(2-3), 2016
PMID: 26433383
New insights into paulomycin biosynthesis pathway in Streptomyces albus J1074 and generation of novel derivatives by combinatorial biosynthesis.
González A, Rodríguez M, Braña AF, Méndez C, Salas JA, Olano C., Microb Cell Fact 15(), 2016
PMID: 27001601
Cell wall glycopolymers of Streptomyces albus, Streptomyces albidoflavus and Streptomyces pathocidini.
Shashkov AS, Streshinskaya GM, Tul'skaya EM, Senchenkova SN, Baryshnikova LM, Dmitrenok AS, Ostash BE, Fedorenko VA., Antonie Van Leeuwenhoek 109(7), 2016
PMID: 27055525

44 References

Daten bereitgestellt von Europe PubMed Central.

KAAS: an automatic genome annotation and pathway reconstruction server.
Moriya Y, Itoh M, Okuda S, Yoshizawa AC, Kanehisa M., Nucleic Acids Res. 35(Web Server issue), 2007
PMID: 17526522
Beta-glucuronidase as a sensitive and versatile reporter in actinomycetes.
Myronovskyi M, Welle E, Fedorenko V, Luzhetskyy A., Appl. Environ. Microbiol. 77(15), 2011
PMID: 21685164
Genome sequence of the streptomycin-producing microorganism Streptomyces griseus IFO 13350.
Ohnishi Y, Ishikawa J, Hara H, Suzuki H, Ikenoya M, Ikeda H, Yamashita A, Hattori M, Horinouchi S., J. Bacteriol. 190(11), 2008
PMID: 18375553
Genome sequence of an industrial microorganism Streptomyces avermitilis: deducing the ability of producing secondary metabolites.
Omura S, Ikeda H, Ishikawa J, Hanamoto A, Takahashi C, Shinose M, Takahashi Y, Horikawa H, Nakazawa H, Osonoe T, Kikuchi H, Shiba T, Sakaki Y, Hattori M., Proc. Natl. Acad. Sci. U.S.A. 98(21), 2001
PMID: 11572948
Emergent properties of reduced-genome Escherichia coli.
Posfai G, Plunkett G 3rd, Feher T, Frisch D, Keil GM, Umenhoffer K, Kolisnychenko V, Stahl B, Sharma SS, de Arruda M, Burland V, Harcum SW, Blattner FR., Science 312(5776), 2006
PMID: 16645050
Replication at the telomeres of the Streptomyces linear plasmid pSLA2.
Qin Z, Cohen SN., Mol. Microbiol. 28(5), 1998
PMID: 9663677
DNA amplifications and deletions in Streptomyces lividans 66 and the loss of one end of the linear chromosome.
Rauland U, Glocker I, Redenbach M, Cullum J., Mol. Gen. Genet. 246(1), 1995
PMID: 7823911
Generation of hybrid elloramycin analogs by combinatorial biosynthesis using genes from anthracycline-type and macrolide biosynthetic pathways.
Rodriguez L, Oelkers C, Aguirrezabalaga I, Brana AF, Rohr J, Mendez C, Salas JA., J. Mol. Microbiol. Biotechnol. 2(3), 2000
PMID: 10937435
Activation and analysis of cryptic crt genes for carotenoid biosynthesis from Streptomyces griseus.
Schumann G, Nurnberger H, Sandmann G, Krugel H., Mol. Gen. Genet. 252(6), 1996
PMID: 8917308
Genetic instability of the Streptomyces chromosome.
Volff JN, Altenbuchner J., Mol. Microbiol. 27(2), 1998
PMID: 9484880
Cloning, sequencing, analysis, and heterologous expression of the fredericamycin biosynthetic gene cluster from Streptomyces griseus.
Wendt-Pienkowski E, Huang Y, Zhang J, Li B, Jiang H, Kwon H, Hutchinson CR, Shen B., J. Am. Chem. Soc. 127(47), 2005
PMID: 16305230
Molecular basis for chloronium-mediated meroterpene cyclization: cloning, sequencing, and heterologous expression of the napyradiomycin biosynthetic gene cluster.
Winter JM, Moffitt MC, Zazopoulos E, McAlpine JB, Dorrestein PC, Moore BS., J. Biol. Chem. 282(22), 2007
PMID: 17392281

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 24337397
PubMed | Europe PMC

Suchen in

Google Scholar