Microscale immobilized enzyme reactors in proteomics: Latest developments

Safdar M, Sproß J, Jaenis J (2014)
Journal of Chromatography A 1324: 1-10.

Es wurde kein Volltext hochgeladen. Nur Publikationsnachweis!
Zeitschriftenaufsatz | Veröffentlicht | Englisch
; ;
Abstract / Bemerkung
Enzymatic digestion of proteins is one of the key steps in proteomic analyses. There has been a steady progress in the applied digestion protocols in the past, starting from conventional time-consuming in-solution or in-gel digestion protocols to rapid and efficient methods utilizing different types of microscale enzyme reactors. Application of such microreactors has been proven beneficial due to lower sample consumption, higher sensitivity and straightforward coupling with LC-MS set-ups. Novel stationary phases, immobilization techniques and device formats are being constantly developed and tested to optimize digestion efficiency of proteolytic enzymes. This review focuses on the latest developments associated with the preparation and application of microscale enzyme reactors for proteomics applications since 2008 onwards. A special attention has been paid to the discussion of different stationary phases applied for immobilization purposes. (C) 2013 Elsevier B.V. All rights reserved.
Journal of Chromatography A


Safdar M, Sproß J, Jaenis J. Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A. 2014;1324:1-10.
Safdar, M., Sproß, J., & Jaenis, J. (2014). Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A, 1324, 1-10. doi:10.1016/j.chroma.2013.11.045
Safdar, M., Sproß, J., and Jaenis, J. (2014). Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A 1324, 1-10.
Safdar, M., Sproß, J., & Jaenis, J., 2014. Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A, 1324, p 1-10.
M. Safdar, J. Sproß, and J. Jaenis, “Microscale immobilized enzyme reactors in proteomics: Latest developments”, Journal of Chromatography A, vol. 1324, 2014, pp. 1-10.
Safdar, M., Sproß, J., Jaenis, J.: Microscale immobilized enzyme reactors in proteomics: Latest developments. Journal of Chromatography A. 1324, 1-10 (2014).
Safdar, Muhammad, Sproß, Jens, and Jaenis, Janne. “Microscale immobilized enzyme reactors in proteomics: Latest developments”. Journal of Chromatography A 1324 (2014): 1-10.

16 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

On-a-chip tryptic digestion of transthyretin: a step toward an integrated microfluidic system for the follow-up of familial transthyretin amyloidosis.
Bataille J, Viodé A, Pereiro I, Lafleur JP, Varenne F, Descroix S, Becher F, Kutter JP, Roesch C, Poüs C, Taverna M, Pallandre A, Smadja C, Le Potier I., Analyst 143(5), 2018
PMID: 29383369
Performance comparison of three trypsin columns used in liquid chromatography.
Šlechtová T, Gilar M, Kalíková K, Moore SM, Jorgenson JW, Tesařová E., J Chromatogr A 1490(), 2017
PMID: 28215403
Use of monolithic supports for high-throughput protein and peptide separation in proteomics.
Andjelković U, Tufegdžić S, Popović M., Electrophoresis 38(22-23), 2017
PMID: 28906564
Advances in monoliths and related porous materials for microfluidics.
Knob R, Sahore V, Sonker M, Woolley AT., Biomicrofluidics 10(3), 2016
PMID: 27190564
A new sample preparation method for the absolute quantitation of a target proteome using (18)O labeling combined with multiple reaction monitoring mass spectrometry.
Li J, Zhou L, Wang H, Yan H, Li N, Zhai R, Jiao F, Hao F, Jin Z, Tian F, Peng B, Zhang Y, Qian X., Analyst 140(4), 2015
PMID: 25568899
Proteomics beyond trypsin.
Tsiatsiani L, Heck AJ., FEBS J 282(14), 2015
PMID: 25823410
Rational synthesis of novel recyclable Fe₃O₄@MOF nanocomposites for enzymatic digestion.
Zhao M, Zhang X, Deng C., Chem Commun (Camb) 51(38), 2015
PMID: 25869528
Nano-LC in proteomics: recent advances and approaches.
Wilson SR, Vehus T, Berg HS, Lundanes E., Bioanalysis 7(14), 2015
PMID: 26270786
Open tubular lab-on-column/mass spectrometry for targeted proteomics of nanogram sample amounts.
Hustoft HK, Vehus T, Brandtzaeg OK, Krauss S, Greibrokk T, Wilson SR, Lundanes E., PLoS One 9(9), 2014
PMID: 25222838

118 References

Daten bereitgestellt von Europe PubMed Central.

Development of an automated digestion and droplet deposition microfluidic chip for MALDI-TOF MS.
Lee J, Musyimi HK, Soper SA, Murray KK., J. Am. Soc. Mass Spectrom. 19(7), 2008
PMID: 18479934
Rapid fabrication of glass/PDMS hybrid µIMER for high throughput membrane proteomics.
Pereira-Medrano AG, Forster S, Fowler GJ, McArthur SL, Wright PC., Lab Chip 10(24), 2010
PMID: 20949197
Proteolysis in microfluidic droplets: an approach to interface protein separation and peptide mass spectrometry.
Ji J, Nie L, Qiao L, Li Y, Guo L, Liu B, Yang P, Girault HH., Lab Chip 12(15), 2012
PMID: 22695710
Facile trypsin immobilization in polymeric membranes for rapid, efficient protein digestion.
Xu F, Wang WH, Tan YJ, Bruening ML., Anal. Chem. 82(24), 2010
PMID: 21087034
Prediction of protein orientation upon immobilization on biological and nonbiological surfaces.
Talasaz AH, Nemat-Gorgani M, Liu Y, Stahl P, Dutton RW, Ronaghi M, Davis RW., Proc. Natl. Acad. Sci. U.S.A. 103(40), 2006
PMID: 17001006
Rapid and enhanced proteolytic digestion using electric-field-oriented enzyme reactor.
Zhou Y, Yi T, Park SS, Chadwick W, Shen RF, Wu WW, Martin B, Maudsley S., J Proteomics 74(7), 2011
PMID: 21338726
Ion-exchange-membrane-based enzyme micro-reactor coupled online with liquid chromatography-mass spectrometry for protein analysis.
Zhou Z, Yang Y, Zhang J, Zhang Z, Bai Y, Liao Y, Liu H., Anal Bioanal Chem 403(1), 2012
PMID: 22349343

Starke, React. Funct. Polym. 73(), 2013

Jagur-Grodzinski, Polym. Adv. Technol. 21(), 2010

Oh, Can. J. Chem. 88(), 2010
Chitosan-based hydrogels for controlled, localized drug delivery.
Bhattarai N, Gunn J, Zhang M., Adv. Drug Deliv. Rev. 62(1), 2010
PMID: 19799949
Digital microfluidic hydrogel microreactors for proteomics.
Luk VN, Fiddes LK, Luk VM, Kumacheva E, Wheeler AR., Proteomics 12(9), 2012
PMID: 22589180
Trypsin-immobilized fiber core in syringe needle for highly efficient proteolysis.
Wang S, Chen Z, Yang P, Chen G., Proteomics 8(9), 2008
PMID: 18442168


Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®


PMID: 24360812
PubMed | Europe PMC

Suchen in

Google Scholar