Optical fluctuation microscopy based on calculating local entropy values

Yahiatene I, Hennig S, Huser T (2013)
Chemical Physics Letters 587: 1-6.

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Journal Article | Original Article | Published | English

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Abstract
We demonstrate a novel and easy-to-use method to dramatically reduce noise and background contributions in advanced fluorescence microscopy experiments. The underlying idea is that the entropy value increases for systems with a large number of accessible energy states. Intensity fluctuations originating from photophysical or photochemical effects lead to an increased information content. Calculating the pixel-wise entropy value results in an enhancement of the signal-to-noise ratio by a factor of 90-100. Comparing ECI (entropy-based contrast-enhanced imaging) to superresolution methods such as STORM and SOFI, we find that this technique also bears substantial potential for enhancing fluctuation-based superresolution microscopies. (C) 2013 Elsevier B. V. All rights reserved.
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Yahiatene I, Hennig S, Huser T. Optical fluctuation microscopy based on calculating local entropy values. Chemical Physics Letters. 2013;587:1-6.
Yahiatene, I., Hennig, S., & Huser, T. (2013). Optical fluctuation microscopy based on calculating local entropy values. Chemical Physics Letters, 587, 1-6. doi:10.1016/j.cplett.2013.08.102
Yahiatene, I., Hennig, S., and Huser, T. (2013). Optical fluctuation microscopy based on calculating local entropy values. Chemical Physics Letters 587, 1-6.
Yahiatene, I., Hennig, S., & Huser, T., 2013. Optical fluctuation microscopy based on calculating local entropy values. Chemical Physics Letters, 587, p 1-6.
I. Yahiatene, S. Hennig, and T. Huser, “Optical fluctuation microscopy based on calculating local entropy values”, Chemical Physics Letters, vol. 587, 2013, pp. 1-6.
Yahiatene, I., Hennig, S., Huser, T.: Optical fluctuation microscopy based on calculating local entropy values. Chemical Physics Letters. 587, 1-6 (2013).
Yahiatene, Idir, Hennig, Simon, and Huser, Thomas. “Optical fluctuation microscopy based on calculating local entropy values”. Chemical Physics Letters 587 (2013): 1-6.
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