Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris

O'Connell A, An S-Q, McCarthy Y, Schulte F, Niehaus K, He Y-Q, Tang J-L, Ryan RP, Dow JM (2013)
Molecular Plant-Microbe Interactions 26(10): 1131-1137.

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Abstract
The black rot pathogen Xanthomonas campestris utilizes molecules of the diffusible signal factor (DSF) family as signals to regulate diverse processes contributing to virulence. DSF signal synthesis and transduction requires proteins encoded by the rpf gene cluster. RpfF catalyzes DSF synthesis, whereas the RpfCG two-component system links the perception of DSF to alteration in the level of the second messenger cyclic di-GMP. As this nucleotide can exert a regulatory influence at the post-transcriptional and post-translational levels, we have used comparative proteomics to identify Rpf-regulated processes in X. campestris that may not be revealed by transcriptomics. The abundance of a number of proteins was altered in rpfF, rpfC, or rpfG mutants compared with the wild type. These proteins belonged to several functional categories, including biosynthesis and intermediary metabolism, regulation, oxidative stress or antibiotic resistance, and DNA replication. For many of these proteins, the alteration in abundance was not associated with alteration in transcript level. A directed mutational analysis allowed us to describe a number of new virulence factors among these proteins, including elongation factor P and a putative outer membrane protein, which are both widely conserved in bacteria.
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O'Connell A, An S-Q, McCarthy Y, et al. Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris. Molecular Plant-Microbe Interactions. 2013;26(10):1131-1137.
O'Connell, A., An, S. - Q., McCarthy, Y., Schulte, F., Niehaus, K., He, Y. - Q., Tang, J. - L., et al. (2013). Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris. Molecular Plant-Microbe Interactions, 26(10), 1131-1137.
O'Connell, A., An, S. - Q., McCarthy, Y., Schulte, F., Niehaus, K., He, Y. - Q., Tang, J. - L., Ryan, R. P., and Dow, J. M. (2013). Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris. Molecular Plant-Microbe Interactions 26, 1131-1137.
O'Connell, A., et al., 2013. Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris. Molecular Plant-Microbe Interactions, 26(10), p 1131-1137.
A. O'Connell, et al., “Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris”, Molecular Plant-Microbe Interactions, vol. 26, 2013, pp. 1131-1137.
O'Connell, A., An, S.-Q., McCarthy, Y., Schulte, F., Niehaus, K., He, Y.-Q., Tang, J.-L., Ryan, R.P., Dow, J.M.: Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris. Molecular Plant-Microbe Interactions. 26, 1131-1137 (2013).
O'Connell, Aileen, An, Shi-Qi, McCarthy, Yvonne, Schulte, Fabian, Niehaus, Karsten, He, Yong-Qiang, Tang, Ji-Liang, Ryan, Robert P., and Dow, J. Maxwell. “Proteomics Analysis of the Regulatory Role of Rpf/DSF Cell-to-Cell Signaling System in the Virulence of Xanthomonas campestris”. Molecular Plant-Microbe Interactions 26.10 (2013): 1131-1137.
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2 Citations in Europe PMC

Data provided by Europe PubMed Central.

The DSF Family of Cell-Cell Signals: An Expanding Class of Bacterial Virulence Regulators.
Ryan RP, An SQ, Allan JH, McCarthy Y, Dow JM., PLoS Pathog. 11(7), 2015
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The PAS domain-containing histidine kinase RpfS is a second sensor for the diffusible signal factor of Xanthomonas campestris.
An SQ, Allan JH, McCarthy Y, Febrer M, Dow JM, Ryan RP., Mol. Microbiol. 92(3), 2014
PMID: 24617591

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