Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*

Heider S, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF (2014)
Applied Microbiology and Biotechnology 98(3): 1223-1235.

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The yellow-pigmented soil bacterium Corynebacterium glutamicum ATCC13032 is accumulating the cyclic C-50 carotenoid decaprenoxanthin and its glucosides. Carotenoid pathway engineering was previously shown to allow for efficient lycopene production. Here, engineering of C. glutamicum for production of endogenous decaprenoxanthin as well as of the heterologous C-50 carotenoids C.p.450 and sarcinaxanthin is described. Plasmid-borne overexpression of genes for lycopene cyclization and hydroxylation from C. glutamicum, Dietzia sp., and Micrococcus luteus, in a lycopene-producing platform strain constructed here, resulted in accumulation of these three C-50 carotenoids to concentrations of about 3-4 mg/g CDW. Chromosomal deletion of a putative carotenoid glycosyltransferase gene cg0730/crtX in these strains entailed production of non-glucosylated derivatives of decaprenoxanthin, C.p.450, and sarcinaxanthin, respectively. Upon introduction of glucosyltransferase genes from M. luteus, C. glutamicum, and Pantoea ananatis, these hydroxylated C-50 carotenoids were glucosylated. We here also demonstrate production of the C-40 carotenoids beta-carotene and zeaxanthin in recombinant C. glutamicum strains and co-expression of the P. ananatis crtX gene was used to obtain glucosylated zeaxanthin. Together, our results show that C. glutamicum is a potentially valuable host for production of a wide range of glucosylated C-40 and C-50 carotenoids.
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Heider S, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF. Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*. Applied Microbiology and Biotechnology. 2014;98(3):1223-1235.
Heider, S., Peters-Wendisch, P., Netzer, R., Stafnes, M., Brautaset, T., & Wendisch, V. F. (2014). Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*. Applied Microbiology and Biotechnology, 98(3), 1223-1235. doi:10.1007/s00253-013-5359-y
Heider, S., Peters-Wendisch, P., Netzer, R., Stafnes, M., Brautaset, T., and Wendisch, V. F. (2014). Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*. Applied Microbiology and Biotechnology 98, 1223-1235.
Heider, S., et al., 2014. Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*. Applied Microbiology and Biotechnology, 98(3), p 1223-1235.
S. Heider, et al., “Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*”, Applied Microbiology and Biotechnology, vol. 98, 2014, pp. 1223-1235.
Heider, S., Peters-Wendisch, P., Netzer, R., Stafnes, M., Brautaset, T., Wendisch, V.F.: Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*. Applied Microbiology and Biotechnology. 98, 1223-1235 (2014).
Heider, Sabine, Peters-Wendisch, Petra, Netzer, Roman, Stafnes, Marit, Brautaset, Trygve, and Wendisch, Volker F. “Production and glucosylation of C50 and C40 carotenoids by metabolically engineered *Corynebacterium glutamicum*”. Applied Microbiology and Biotechnology 98.3 (2014): 1223-1235.
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12 Citations in Europe PMC

Data provided by Europe PubMed Central.

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