Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence

Unger A, Brunne B, Hinssen H (2013)
Archives Of Biochemistry And Biophysics 536(1): 38-45.

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Two distinct isoforms of the Ca-dependent actin filament severing protein gelsolin were identified in cross-striated muscles of the American lobster. The variants (termed LG1 and LG2) differ by an extension of 18 AA at the C-terminus of LG1, and by two substitutions at AA735 and AA736, the two C-terminal amino acids of LG2. Functional comparison of the isolated and purified proteins revealed gelsolin-typical properties for both with differences in Ca2+-sensitivity, LG2 being activated at significant lower Ca-concentration than LG1: Half maximal activation for both filament severing and G-actin binding was similar to 4 x 10(-7) M Ca2+ for LG2 vs. similar to 2 x 10(-6) M Ca2+ for LG1. This indicates a differential activation for the two isoproteins in vivo where they are present in almost equal amounts in the muscle cell. Structure prediction modeling on the basis of the known structure of mammalian gelsolin shows that LG2 lacks the C-terminal alpha-helix which is involved in contact formation between domains G6 and G2. In both mammalian gelsolin and LG1, this "latch bridge" is assumed to play a critical role in Ca2+-activation by keeping gelsolin in a closed, inactive conformation at low [Ca2+]. In LG2, the reduced contact between G6 and G2 may be responsible for its activation at low Ca2+-concentration. (C) 2013 Elsevier Inc. All rights reserved.
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Unger A, Brunne B, Hinssen H. Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence. Archives Of Biochemistry And Biophysics. 2013;536(1):38-45.
Unger, A., Brunne, B., & Hinssen, H. (2013). Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence. Archives Of Biochemistry And Biophysics, 536(1), 38-45.
Unger, A., Brunne, B., and Hinssen, H. (2013). Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence. Archives Of Biochemistry And Biophysics 536, 38-45.
Unger, A., Brunne, B., & Hinssen, H., 2013. Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence. Archives Of Biochemistry And Biophysics, 536(1), p 38-45.
A. Unger, B. Brunne, and H. Hinssen, “Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence”, Archives Of Biochemistry And Biophysics, vol. 536, 2013, pp. 38-45.
Unger, A., Brunne, B., Hinssen, H.: Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence. Archives Of Biochemistry And Biophysics. 536, 38-45 (2013).
Unger, Andreas, Brunne, Bianka, and Hinssen, Horst. “Isoforms of gelsolin from lobster striated muscles differ in Calcium-dependence”. Archives Of Biochemistry And Biophysics 536.1 (2013): 38-45.
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