Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines

Viefhues M, Wegener S, Rischmueller A, Schleef M, Anselmetti D (2013)
Lab On A Chip 13(15): 3111-3118.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
We present a prototype nanofluidic device, developed for the continuous-flow dielectrophoretic (DEP) fractionation, purification, and quality control of sample suspensions for gene vaccine production. The device consists of a cross injector, two operation regions, and separate outlets where the analytes are collected. In each DEP operation region, an inhomogeneous electric field is generated at a channel spanning insulating ridge. The samples are driven by ac and dc voltages that generate a dielectrophoretic potential at the ridge as well as (linear) electrokinetics. Since the DEP potential differs at the two ridges, probes of three and more species can be iteratively fully fractionated. We demonstrate the fast and efficient separation of parental plasmid, miniplasmid, and minicircle DNA, where the latter is applicable as a gene vaccine. Since the present technique is virtually label-free, it offers a fast purification and in-process quality control with low consumption, in parallel, for the production of gene vaccines.
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Zeitschriftentitel
Lab On A Chip
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13
Zeitschriftennummer
15
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3111-3118
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Viefhues M, Wegener S, Rischmueller A, Schleef M, Anselmetti D. Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab On A Chip. 2013;13(15):3111-3118.
Viefhues, M., Wegener, S., Rischmueller, A., Schleef, M., & Anselmetti, D. (2013). Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab On A Chip, 13(15), 3111-3118. doi:10.1039/c3lc50475a
Viefhues, M., Wegener, S., Rischmueller, A., Schleef, M., and Anselmetti, D. (2013). Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab On A Chip 13, 3111-3118.
Viefhues, M., et al., 2013. Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab On A Chip, 13(15), p 3111-3118.
M. Viefhues, et al., “Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines”, Lab On A Chip, vol. 13, 2013, pp. 3111-3118.
Viefhues, M., Wegener, S., Rischmueller, A., Schleef, M., Anselmetti, D.: Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines. Lab On A Chip. 13, 3111-3118 (2013).
Viefhues, Martina, Wegener, Sonja, Rischmueller, Anja, Schleef, Martin, and Anselmetti, Dario. “Dielectrophoresis based continuous-flow nano sorter: fast quality control of gene vaccines”. Lab On A Chip 13.15 (2013): 3111-3118.

8 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

DNA dielectrophoresis: Theory and applications a review.
Viefhues M, Eichhorn R., Electrophoresis 38(11), 2017
PMID: 28306161
Reaching for the limits in continuous-flow dielectrophoretic DNA analysis.
Täuber S, Kunze L, Grauberger O, Grundmann A, Viefhues M., Analyst 142(24), 2017
PMID: 29119187
The development of malaria diagnostic techniques: a review of the approaches with focus on dielectrophoretic and magnetophoretic methods.
Kasetsirikul S, Buranapong J, Srituravanich W, Kaewthamasorn M, Pimpin A., Malar J 15(1), 2016
PMID: 27405995
Dielectrophoretic concentrator enhancement based on dielectric poles for continuously flowing samples.
del Moral Zamora B, Álvarez Azpeitia JM, Oliva Brañas AM, Colomer-Farrarons J, Castellarnau M, Miribel-Català PL, Homs-Corbera A, Juárez A, Samitier J., Electrophoresis 36(13), 2015
PMID: 25630478
Dielectrophoretic separation of bioparticles in microdevices: a review.
Jubery TZ, Srivastava SK, Dutta P., Electrophoresis 35(5), 2014
PMID: 24338825
Dielectrophoretic separation of micron and submicron particles: a review.
Dash S, Mohanty S., Electrophoresis 35(18), 2014
PMID: 24930837
Dielectrophoresis for bioparticle manipulation.
Qian C, Huang H, Chen L, Li X, Ge Z, Chen T, Yang Z, Sun L., Int J Mol Sci 15(10), 2014
PMID: 25310652

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