Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology

Baumgart M, Unthan S, Rückert C, Sivalingam J, Grünberger A, Kalinowski J, Bott M, Noack S, Frunzke J (2013)
Applied and environmental microbiology 79(19): 6006-6015.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
: The activity of bacteriophages and phage-related mobile elements is a major source for genome rearrangements and genetic instability of their bacterial hosts. The genome of the industrial amino acid producer Corynebacterium glutamicum ATCC 13032 contains three prophages (CGP1-3) of so far unknown functionality. Several phage genes are regularly expressed and the large prophage CGP3 (∼190 kbp) has recently been shown to be induced under certain stress conditions. Here, we present the construction of MB001, a prophage-free variant of C. glutamicum ATCC 13032 with a 6 % reduced genome. This strain does not show any unfavorable properties during extensive phenotypic characterization under various standard and stress conditions. As expected, we observed an improved growth and fitness of MB001 under SOS-response inducing conditions that trigger CGP3 induction in the wild type strain. Further studies revealed that MB001 has a significantly increased transformation efficiency and produced about 30 % more of the heterologous model protein eYFP, presumably as a consequence of an increased plasmid copy number. These effects were attributed to the loss of the restriction-modification system (cg1996-98) located within CGP3. The deletion of the prophages without any negative effect results in a novel platform strain for metabolic engineering and represents a useful step towards the construction of a C. glutamicum chassis genome of strain ATCC 13032 for biotechnological applications and synthetic biology.
Publishing Year
ISSN
PUB-ID

Cite this

Baumgart M, Unthan S, Rückert C, et al. Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology. Applied and environmental microbiology. 2013;79(19):6006-6015.
Baumgart, M., Unthan, S., Rückert, C., Sivalingam, J., Grünberger, A., Kalinowski, J., Bott, M., et al. (2013). Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology. Applied and environmental microbiology, 79(19), 6006-6015.
Baumgart, M., Unthan, S., Rückert, C., Sivalingam, J., Grünberger, A., Kalinowski, J., Bott, M., Noack, S., and Frunzke, J. (2013). Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology. Applied and environmental microbiology 79, 6006-6015.
Baumgart, M., et al., 2013. Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology. Applied and environmental microbiology, 79(19), p 6006-6015.
M. Baumgart, et al., “Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology”, Applied and environmental microbiology, vol. 79, 2013, pp. 6006-6015.
Baumgart, M., Unthan, S., Rückert, C., Sivalingam, J., Grünberger, A., Kalinowski, J., Bott, M., Noack, S., Frunzke, J.: Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology. Applied and environmental microbiology. 79, 6006-6015 (2013).
Baumgart, Meike, Unthan, Simon, Rückert, Christian, Sivalingam, Jasintha, Grünberger, Alexander, Kalinowski, Jörn, Bott, Michael, Noack, Stephan, and Frunzke, Julia. “Construction of a prophage-free variant of Corynebacterium glutamicum ATCC 13032 - a platform strain for basic research and industrial biotechnology”. Applied and environmental microbiology 79.19 (2013): 6006-6015.
This data publication is cited in the following publications:
This publication cites the following data publications:

15 Citations in Europe PMC

Data provided by Europe PubMed Central.

Production of the Marine Carotenoid Astaxanthin by Metabolically Engineered Corynebacterium glutamicum.
Henke NA, Heider SA, Peters-Wendisch P, Wendisch VF., Mar Drugs 14(7), 2016
PMID: 27376307
Genome sequence of the soil bacterium Corynebacterium callunae type strain DSM 20147(T).
Persicke M, Albersmeier A, Bednarz H, Niehaus K, Kalinowski J, Ruckert C., Stand Genomic Sci 10(), 2015
PMID: 26203323
Genome-based identification of active prophage regions by next generation sequencing in Bacillus licheniformis DSM13.
Hertel R, Rodriguez DP, Hollensteiner J, Dietrich S, Leimbach A, Hoppert M, Liesegang H, Volland S., PLoS ONE 10(3), 2015
PMID: 25811873
Chassis organism from Corynebacterium glutamicum--a top-down approach to identify and delete irrelevant gene clusters.
Unthan S, Baumgart M, Radek A, Herbst M, Siebert D, Bruhl N, Bartsch A, Bott M, Wiechert W, Marin K, Hans S, Kramer R, Seibold G, Frunzke J, Kalinowski J, Ruckert C, Wendisch VF, Noack S., Biotechnol J 10(2), 2015
PMID: 25139579
Optimization of the IPP Precursor Supply for the Production of Lycopene, Decaprenoxanthin and Astaxanthin by Corynebacterium glutamicum.
Heider SA, Wolf N, Hofemeier A, Peters-Wendisch P, Wendisch VF., Front Bioeng Biotechnol 2(), 2014
PMID: 25191655
Clostridium difficile phages: still difficult?
Hargreaves KR, Clokie MR., Front Microbiol 5(), 2014
PMID: 24808893
Analysis of SOS-induced spontaneous prophage induction in Corynebacterium glutamicum at the single-cell level.
Nanda AM, Heyer A, Kramer C, Grunberger A, Kohlheyer D, Frunzke J., J. Bacteriol. 196(1), 2014
PMID: 24163339

59 References

Data provided by Europe PubMed Central.

Characterization of an endogenous plasmid and development of cloning vectors and a transformation system in
Santamaria R, Gil JA, Mesas JM, Martin JF., 1984
Cryptic plasmids in glutamic acid-producing bacteria
Miwa K, Matsui H, Terabe M, Nakamori S, Sano K, Momose H., 1984
Replication and control of circular bacterial plasmids.
del Solar G, Giraldo R, Ruiz-Echevarria MJ, Espinosa M, Diaz-Orejas R., Microbiol. Mol. Biol. Rev. 62(2), 1998
PMID: 9618448
The family of highly interrelated single-stranded deoxyribonucleic acid plasmids.
Gruss A, Ehrlich SD., Microbiol. Rev. 53(2), 1989
PMID: 2666843
Plasmid copy number control: an ever-growing story.
del Solar G, Espinosa M., Mol. Microbiol. 37(3), 2000
PMID: 10931343
The nucleotide sequence of pACYC184.
Rose RE., Nucleic Acids Res. 16(1), 1988
PMID: 3340533
Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum.
Hentschel E, Will C, Mustafi N, Burkovski A, Rehm N, Frunzke J., Microb Biotechnol 6(2), 2013
PMID: 22938655

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 23892752
PubMed | Europe PMC

Search this title in

Google Scholar