RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens

Vorhölter F-J (2013)
Molecular Microbiology 88(6): 1041-1046.

Journal Article | Published | English

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Abstract
RNA-Seq is opening new doors for the functional understanding of microorganisms. Advances in RNA-Seq technology are allowing investigators to focus their studies on specific functional questions. An interesting example is presented by An etal. (2013) in this issue of Molecular Microbiology. New genes were identified for proteins and ncRNAs when the authors concentrated on the role of the rpf genes, which code for key components of a signal transduction hub in the plant pathogen Xanthomonas campestris pv. campestris. Although rpf gene products were already known to be involved in controlling transcription of many genes, including those encoding several important virulence factors, novel and unexpected properties of this signal transduction system emerged from the RNA-Seq analysis. In addition to identifying new target genes influenced by the rpf genes, the study found that the regulons of RpfC and RpfG, the sensor and response regulator of the master two-component regulatory system, only partially overlapped, indicating that the Rpf signalling system is even more complex than previously appreciated.
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Vorhölter F-J. RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens. Molecular Microbiology. 2013;88(6):1041-1046.
Vorhölter, F. - J. (2013). RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens. Molecular Microbiology, 88(6), 1041-1046.
Vorhölter, F. - J. (2013). RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens. Molecular Microbiology 88, 1041-1046.
Vorhölter, F.-J., 2013. RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens. Molecular Microbiology, 88(6), p 1041-1046.
F.-J. Vorhölter, “RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens”, Molecular Microbiology, vol. 88, 2013, pp. 1041-1046.
Vorhölter, F.-J.: RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens. Molecular Microbiology. 88, 1041-1046 (2013).
Vorhölter, Frank-Jörg. “RNA-Seq facilitates a new perspective on signal transduction and gene regulation in important plant pathogens”. Molecular Microbiology 88.6 (2013): 1041-1046.
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2 Citations in Europe PMC

Data provided by Europe PubMed Central.

The DSF Family of Cell-Cell Signals: An Expanding Class of Bacterial Virulence Regulators.
Ryan RP, An SQ, Allan JH, McCarthy Y, Dow JM., PLoS Pathog. 11(7), 2015
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Proteomics analysis of the regulatory role of Rpf/DSF cell-to-cell signaling system in the virulence of Xanthomonas campestris.
O'Connell A, An SQ, McCarthy Y, Schulte F, Niehaus K, He YQ, Tang JL, Ryan RP, Dow JM., Mol. Plant Microbe Interact. 26(10), 2013
PMID: 23819805

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