Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1

Niu B, Vater J, Rückert C, Blom J, Lehmann M, Ru J-J, Chen X-H, Wang Q, Borriss R (2013)
BMC microbiology 13(1): 137.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ;
Abstract
BACKGROUND: Nine gene clusters dedicated to nonribosomal synthesis of secondary metabolites with possible antimicrobial action, including polymyxin and fusaricidin, were detected within the whole genome sequence of the plant growth-promoting rhizobacterium (PGPR) Paenibacillus polymyxa M-1. To survey the antimicrobial compounds expressed by M-1 we analyzed the active principle suppressing phytopathogenic Erwinia spp. RESULTS: P. polymyxa M-1 suppressed the growth of phytopathogenic Erwinia amylovora Ea 273, and E. carotovora, the causative agents of fire blight and soft rot, respectively. By MALDI-TOF mass spectrometry and reversed-phase high-performance liquid chromatography (RP-HPLC), two antibacterial compounds bearing molecular masses of 1190.9 Da and 1176.9 Da were detected as being the two components of polymyxin P, polymyxin P1 and P2, respectively. The active principle acting against the two Erwinia strains was isolated from TLC plates and identified by postsource decay (PSD)-MALDI-TOF mass spectrometry as polymyxin P1 and polymyxin P2. These findings were corroborated by domain structure analysis of the polymyxin (pmx) gene cluster detected in the M-1 chromosome which revealed that corresponding to the chemical structure of polymyxin P, the gene cluster is encoding D-Phe in position 6 and L-Thr in position 7. CONCLUSIONS: Identical morphological changes in the cell wall of the bacterial phytopathogens treated with either crude polymyxin P or culture supernatant of M-1 corroborated that polymyxin P is the main component of the biocontrol effect exerted by strain M-1 against phytopathogenic Erwinia spp.
Publishing Year
ISSN
PUB-ID

Cite this

Niu B, Vater J, Rückert C, et al. Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology. 2013;13(1):137.
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J. - J., Chen, X. - H., et al. (2013). Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology, 13(1), 137. doi:10.1186/1471-2180-13-137
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J. - J., Chen, X. - H., Wang, Q., and Borriss, R. (2013). Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology 13, 137.
Niu, B., et al., 2013. Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology, 13(1), p 137.
B. Niu, et al., “Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1”, BMC microbiology, vol. 13, 2013, pp. 137.
Niu, B., Vater, J., Rückert, C., Blom, J., Lehmann, M., Ru, J.-J., Chen, X.-H., Wang, Q., Borriss, R.: Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1. BMC microbiology. 13, 137 (2013).
Niu, Ben, Vater, Joachim, Rückert, Christian, Blom, Jochen, Lehmann, Maik, Ru, Jin-Jiang, Chen, Xiao-Hua, Wang, Qi, and Borriss, Rainer. “Polymyxin P is the active principle in suppressing phytopathogenic Erwinia spp. by the biocontrol rhizobacterium Paenibacillus polymyxa M-1”. BMC microbiology 13.1 (2013): 137.
This data publication is cited in the following publications:
This publication cites the following data publications:

16 Citations in Europe PMC

Data provided by Europe PubMed Central.

Fusaricidins from Paenibacillus polymyxa M-1, a family of lipohexapeptides of unusual complexity-a mass spectrometric study.
Vater J, Herfort S, Doellinger J, Weydmann M, Dietel K, Faetke S, Lasch P., J Mass Spectrom 52(1), 2017
PMID: 27714901
Dynamics of bacterial class Bacilli in the deepest valley lake of Kashmir-the Manasbal Lake.
Shafi S, Kamili AN, Shah MA, Bandh SA, Dar R., Microb Pathog 104(), 2017
PMID: 28087491
Seeds of the Wild Progenitor of Maize Possess Bacteria That Antagonize Foodborne Pathogens.
Shehata HR, Griffiths MW, Raizada MN., Foodborne Pathog Dis 14(4), 2017
PMID: 28186422
Simplified and representative bacterial community of maize roots.
Niu B, Paulson JN, Zheng X, Kolter R., Proc Natl Acad Sci U S A 114(12), 2017
PMID: 28275097
The antibacterial activity and modes of LI-F type antimicrobial peptides against Bacillus cereus in vitro.
Han J, Zhao S, Ma Z, Gao L, Liu H, Muhammad U, Lu Z, Lv F, Bie X., J Appl Microbiol 123(3), 2017
PMID: 28650559
Draft Genome Sequence of Bacillus cereus 905, a Plant Growth-Promoting Rhizobacterium of Wheat.
Ding H, Niu B, Fan H, Li Y, Wang Q., Genome Announc 4(3), 2016
PMID: 27257205
Genome mining: Prediction of lipopeptides and polyketides from Bacillus and related Firmicutes.
Aleti G, Sessitsch A, Brader G., Comput Struct Biotechnol J 13(), 2015
PMID: 25893081
Full genome sequence of Brevibacillus laterosporus strain B9, a biological control strain isolated from Zhejiang, China.
Li G, Xu J, Wu L, Ren D, Ye W, Dong G, Zhu L, Zeng D, Guo L., J Biotechnol 207(), 2015
PMID: 26022423
Characterization of Novel Fusaricidins Produced by Paenibacillus polymyxa-M1 Using MALDI-TOF Mass Spectrometry.
Vater J, Niu B, Dietel K, Borriss R., J Am Soc Mass Spectrom 26(9), 2015
PMID: 26100395
Complete Genome Sequence of Paenibacillus polymyxa SQR-21, a Plant Growth-Promoting Rhizobacterium with Antifungal Activity and Rhizosphere Colonization Ability.
Li S, Yang D, Qiu M, Shao J, Guo R, Shen B, Yin X, Zhang R, Zhang N, Shen Q., Genome Announc 2(2), 2014
PMID: 24723719

51 References

Data provided by Europe PubMed Central.

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 23773687
PubMed | Europe PMC

Search this title in

Google Scholar