Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*

Vold Korgaard Jensen J, Wendisch VF (2013)
Microbial Cell Factories 12(63).

Download
OA
Zeitschriftenaufsatz | Veröffentlicht | Englisch
Volltext vorhanden für diesen Nachweis
Abstract / Bemerkung
Background The soil bacterium Corynebacterium glutamicum, best known for its glutamate producing ability, is suitable as a producer of a variety of bioproducts. Glutamate is the precursor of the amino acid proline. Proline biosynthesis typically involves three enzymes and a spontaneous cyclisation reaction. Alternatively, proline can be synthesised from ornithine, an intermediate of arginine biosynthesis. The direct conversion of ornithine to proline is catalysed by ornithine cyclodeaminase. An ornithine overproducing platform strain with deletions of argR and argF (ORN1) has been employed for production of derived compounds such as putrescine. By heterologous expression of ocd this platform strain can be engineered further for proline production. Results Plasmid-based expression of ocd encoding the putative ornithine cyclodeaminase of C. glutamicum did not result in detectable proline accumulation in the culture medium. However, plasmid-based expression of ocd from Pseudomonas putida resulted in proline production with yields up to 0.31 ± 0.01 g proline/g glucose. Overexpression of the gene encoding a feedback-alleviated N-acetylglutamate kinase further increased proline production to 0.36 ± 0.01 g/g. In addition, feedback-alleviation of N-acetylglutamate kinase entailed growth-coupled production of proline and reduced the accumulation of by-products in the culture medium. Conclusions The product spectrum of the platform strain C. glutamicum ORN1 was expanded to include the amino acid L-proline. Upon further development of the ornithine overproducing platform strain, industrial production of amino acids of the glutamate family and derived bioproducts such as diamines might become within reach.
Erscheinungsjahr
Zeitschriftentitel
Microbial Cell Factories
Band
12
Zeitschriftennummer
63
ISSN
PUB-ID

Zitieren

Vold Korgaard Jensen J, Wendisch VF. Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*. Microbial Cell Factories. 2013;12(63).
Vold Korgaard Jensen, J., & Wendisch, V. F. (2013). Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*. Microbial Cell Factories, 12(63). doi:10.1186/1475-2859-12-63
Vold Korgaard Jensen, J., and Wendisch, V. F. (2013). Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*. Microbial Cell Factories 12.
Vold Korgaard Jensen, J., & Wendisch, V.F., 2013. Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*. Microbial Cell Factories, 12(63).
J. Vold Korgaard Jensen and V.F. Wendisch, “Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*”, Microbial Cell Factories, vol. 12, 2013.
Vold Korgaard Jensen, J., Wendisch, V.F.: Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*. Microbial Cell Factories. 12, (2013).
Vold Korgaard Jensen, Jaide, and Wendisch, Volker F. “Ornithine cyclodeaminase-based proline production by *Corynebacterium glutamicum*”. Microbial Cell Factories 12.63 (2013).
Alle Dateien verfügbar unter der/den folgenden Lizenz(en):
Copyright Statement:
This Item is protected by copyright and/or related rights. [...]
Volltext(e)
Access Level
OA Open Access
Zuletzt Hochgeladen
2016-08-23T11:40:07Z

27 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Metabolic engineering of Corynebacterium glutamicum for 2-ketoisocaproate production.
Bückle-Vallant V, Krause FS, Messerschmidt S, Eikmanns BJ., Appl Microbiol Biotechnol 98(1), 2014
PMID: 24169948
Engineering biotin prototrophic Corynebacterium glutamicum strains for amino acid, diamine and carotenoid production.
Peters-Wendisch P, Götker S, Heider SA, Komati Reddy G, Nguyen AQ, Stansen KC, Wendisch VF., J Biotechnol 192 Pt B(), 2014
PMID: 24486440

42 References

Daten bereitgestellt von Europe PubMed Central.

L-Glutamine as a nitrogen source for Corynebacterium glutamicum: derepression of the AmtR regulon and implications for nitrogen sensing.
Rehm N, Georgi T, Hiery E, Degner U, Schmiedl A, Burkovski A, Bott M., Microbiology (Reading, Engl.) 156(Pt 10), 2010
PMID: 20656783
Enhancement of ornithine production in proline-supplemented Corynebacterium glutamicum by ornithine cyclodeaminase.
Lee SY, Cho JY, Lee HJ, Kim YH, Min J., J. Microbiol. Biotechnol. 20(1), 2010
PMID: 20134243
The plant oncogene rolD encodes a functional ornithine cyclodeaminase.
Trovato M, Maras B, Linhares F, Costantino P., Proc. Natl. Acad. Sci. U.S.A. 98(23), 2001
PMID: 11687622
The Noc region of Ti plasmid C58 codes for arginase and ornithine cyclodeaminase.
Sans N, Schroder G, Schroder J., Eur. J. Biochem. 167(1), 1987
PMID: 3040404
Codon usage patterns in Corynebacterium glutamicum: mutational bias, natural selection and amino acid conservation
AUTHOR UNKNOWN, 2010
Taxonomical studies on glutamic acid producing bacteria
AUTHOR UNKNOWN, 1967
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791

AUTHOR UNKNOWN, 1989
Experiments
AUTHOR UNKNOWN, 2005
The design of optimum multifactorial experiments
AUTHOR UNKNOWN, 1946
Chapter 3: Two-level factorial design
AUTHOR UNKNOWN, 2007

Export

Markieren/ Markierung löschen
Markierte Publikationen

Open Data PUB

Web of Science

Dieser Datensatz im Web of Science®

Quellen

PMID: 23806148
PubMed | Europe PMC

Suchen in

Google Scholar