A laser-supported lowerable surface setup to study the role of ground contact during stepping

Berendes V, Dübbert M, Bockemühl T, Schmitz J, Büschges A, Gruhn M (2013)
Journal of neuroscience methods 215(2): 224-233.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
We introduce a laser-supported setup to study the influence of afferent input on muscle activation during walking, using a movable ground platform. This approach allows investigating if and how the activity of stance phase muscles of an insect (e.g. stick insect) responds to a missing ground contact signal. The walking surface consists of a fixed and a lowerable part, which can be lowered to defined levels below the previous ground level at any time point during a walking sequence. As a consequence, the leg under investigation finds either a lower ground level or no ground support at all. The lowerable walking surface consists of a 49mm×34mm stainless steel surface, made slippery and equipped for tarsal contact monitoring, similar to the system that was described by Gruhn and colleagues (Gruhn et al., 2006). The setup controller allows pneumatic lowering of the surface and subsequent detection of tarsal entry into the previous ground level with the help of a thin sheet of laser light and a corresponding detector. Here, we describe basic properties of the new setup and show the results of first experiments to demonstrate its use for the study of sensory and central influences in stepping of a small animal. In the experiments, we compare the effect of ground-support ("control") with either steps into the hole (SiH), ground support at a lower surface level, or the amputation of the tarsus on the onset of EMG activity in the flexor tibiae muscle of the stick insect.
Publishing Year
ISSN
PUB-ID

Cite this

Berendes V, Dübbert M, Bockemühl T, Schmitz J, Büschges A, Gruhn M. A laser-supported lowerable surface setup to study the role of ground contact during stepping. Journal of neuroscience methods. 2013;215(2):224-233.
Berendes, V., Dübbert, M., Bockemühl, T., Schmitz, J., Büschges, A., & Gruhn, M. (2013). A laser-supported lowerable surface setup to study the role of ground contact during stepping. Journal of neuroscience methods, 215(2), 224-233.
Berendes, V., Dübbert, M., Bockemühl, T., Schmitz, J., Büschges, A., and Gruhn, M. (2013). A laser-supported lowerable surface setup to study the role of ground contact during stepping. Journal of neuroscience methods 215, 224-233.
Berendes, V., et al., 2013. A laser-supported lowerable surface setup to study the role of ground contact during stepping. Journal of neuroscience methods, 215(2), p 224-233.
V. Berendes, et al., “A laser-supported lowerable surface setup to study the role of ground contact during stepping”, Journal of neuroscience methods, vol. 215, 2013, pp. 224-233.
Berendes, V., Dübbert, M., Bockemühl, T., Schmitz, J., Büschges, A., Gruhn, M.: A laser-supported lowerable surface setup to study the role of ground contact during stepping. Journal of neuroscience methods. 215, 224-233 (2013).
Berendes, Volker, Dübbert, Michael, Bockemühl, Till, Schmitz, Joscha, Büschges, Ansgar, and Gruhn, Matthias. “A laser-supported lowerable surface setup to study the role of ground contact during stepping”. Journal of neuroscience methods 215.2 (2013): 224-233.
This data publication is cited in the following publications:
This publication cites the following data publications:

1 Citation in Europe PMC

Data provided by Europe PubMed Central.

The role of leg touchdown for the control of locomotor activity in the walking stick insect.
Schmitz J, Gruhn M, Buschges A., J. Neurophysiol. 113(7), 2015
PMID: 25652931

48 References

Data provided by Europe PubMed Central.

Coordinated walking of stick insects on a mercury surface
Graham, J Exp Biol 92(), 1981
Adaptive changes in locomotor control after partial denervation of triceps surae muscles in the cat.
Gritsenko V, Mushahwar V, Prochazka A., J. Physiol. (Lond.) 533(Pt 1), 2001
PMID: 11351036
Tethered stick insect walking: a modified slippery surface setup with optomotor stimulation and electrical monitoring of tarsal contact.
Gruhn M, Hoffmann O, Dubbert M, Scharstein H, Buschges A., J. Neurosci. Methods 158(2), 2006
PMID: 16824615
Straight walking and turning on a slippery surface.
Gruhn M, Zehl L, Buschges A., J. Exp. Biol. 212(Pt 2), 2009
PMID: 19112138

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Distribution of central pattern generators for rhythmic motor outputs in the spinal cord of limbed vertebrates
Kiehn, Ann NY Acad Sci 860(), 1998
Leg adjustments during running across visible and camouflaged incidental changes in ground level.
Muller R, Ernst M, Blickhan R., J. Exp. Biol. 215(Pt 17), 2012
PMID: 22875771
Three forms of the scratch reflex in the spinal turtle: central generation of motor patterns.
Robertson GA, Mortin LI, Keifer J, Stein PS., J. Neurophysiol. 53(6), 1985
PMID: 4009231
Rhythmic activity in a motor axon induced by axotomy.
Schmidt J, Grund M., Neuroreport 14(9), 2003
PMID: 12824773
Properties of the feedback system controlling the coxa-trochanter-joint in the stick insect Carausius morosus
Schmitz, Biol Cybern 55(), 1986
Load-compensating reactions in the proximal leg joints of stick insects during standing and walking
Schmitz, J Exp Biol 183(), 1993
Intersegmental transfer of sensory signals in the stick insect leg muscle control system.
Stein W, Buschges A, Bassler U., J. Neurobiol. 66(11), 2006
PMID: 16902990
Patterns of spinal sensory-motor connectivity prescribed by a dorsoventral positional template.
Surmeli G, Akay T, Ippolito GC, Tucker PW, Jessell TM., Cell 147(3), 2011
PMID: 22036571
Laufen und Stehen der Stabheuschrecke Carausius morosus: Sinnesborstenfelder in den Beingelenken als Glieder von Regelkreisen
Wendler, Z Vergl Physiol 48(), 1964
Erzeugung und Kontrolle koordinierter Bewegungen bei Tieren—Beispiele an Insekten
Wendler, Kybernetik, München: Oldenbourg (), 1977
Encoding of force increases and decreases by tibial campaniform sensilla in the stick insect, Carausius morosus.
Zill SN, Buschges A, Schmitz J., J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 197(8), 2011
PMID: 21544617

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 23562598
PubMed | Europe PMC

Search this title in

Google Scholar