Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles

Dimitrov V, Kakorin S, Neumann E (2013)
Physical Chemistry Chemical Physics 15(17): 6303-6322.

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Abstract
The results of electrooptical and conductometrical measurements on unilamellar lipid vesicles (of mean radius a = 90 nm), filled with 0.2 M NaCl solution, suspended in 0.33 M sucrose solution of 0.2 mM NaCl, and exposed to a stepwise decaying electric field (time constant tau(E) = 154 mu s) in the range 10 <= E-0 (kV cm(-1)) <= 90, are analyzed in terms of cyclic changes in vesicle shape and vesicle membrane conductivity. The two peaks in the dichroitic turbidity relaxations reflect two cycles of rapid membrane electroporation and slower resealing of long-lived electropores. The field-induced changes reflect structural transitions between closed (C) and porated (P) membrane states, qualified by pores of type P-1 and of type P-2, respectively. The transient change in the membrane conductivity and the transient shape oscillation are based on changes in the pore density of the (larger) P-2-pores along a hysteresis cycle. The P-2-pore formation leads to transient net ion flows across the P-2-pores and to transient changes in the membrane field. The kinetic data are numerically processed in terms of coupled structural relaxation modes. Using the torus-hole pore model, the mean inner pore radii are estimated to be r(1) = 0.38 (+/- 0.05) nm and r(2) = 1.7 (+/- 0.1) nm, respectively. The observation of a transient oscillation of membrane electroporation and of shape changes in a longer lasting external field pulse is suggestive of potential resonance enhancement, for instance, of electro-uptake by, and of electro-release of biogenic molecules from, biological cells in trains of long-lasting low-intensity voltage pulses.
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Dimitrov V, Kakorin S, Neumann E. Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles. Physical Chemistry Chemical Physics. 2013;15(17):6303-6322.
Dimitrov, V., Kakorin, S., & Neumann, E. (2013). Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles. Physical Chemistry Chemical Physics, 15(17), 6303-6322.
Dimitrov, V., Kakorin, S., and Neumann, E. (2013). Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles. Physical Chemistry Chemical Physics 15, 6303-6322.
Dimitrov, V., Kakorin, S., & Neumann, E., 2013. Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles. Physical Chemistry Chemical Physics, 15(17), p 6303-6322.
V. Dimitrov, S. Kakorin, and E. Neumann, “Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles”, Physical Chemistry Chemical Physics, vol. 15, 2013, pp. 6303-6322.
Dimitrov, V., Kakorin, S., Neumann, E.: Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles. Physical Chemistry Chemical Physics. 15, 6303-6322 (2013).
Dimitrov, Vasil, Kakorin, Sergej, and Neumann, Eberhard. “Transient oscillation of shape and membrane conductivity changes by field pulse-induced electroporation in nano-sized phospholipid vesicles”. Physical Chemistry Chemical Physics 15.17 (2013): 6303-6322.
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45 References

Data provided by Europe PubMed Central.

Light scattering by a core-mantle spheroidal particle.
Farafonov VG, Voshchinnikov NV, Somsikov VV., Appl Opt 35(27), 1996
PMID: 21127540

Sersa, Bioelectrochem. Bioenerg. 43(), 1997

Kakorin, Ber. Bunsenges. Phys. Chem. 100(), 1996

Kakorin, Coll. Surf. A. 209(), 2002

Neumann, Faraday Discuss. 111(), 1998
Conformation changes in rRNA induced by electric impulses.
Revzin A, Neumann E., Biophys. Chem. 2(2), 1974
PMID: 4611518
Electrical properties of tissue and cell suspensions.
SCHWAN HP., Adv Biol Med Phys 5(), 1957
PMID: 13520431
Ion transport across transmembrane pores.
Leontiadou H, Mark AE, Marrink SJ., Biophys. J. 92(12), 2007
PMID: 17384063
Kinetics, statistics, and energetics of lipid membrane electroporation studied by molecular dynamics simulations.
Bockmann RA, de Groot BL, Kakorin S, Neumann E, Grubmuller H., Biophys. J. 95(4), 2008
PMID: 18469089

Fricke, J. Appl. Phys. 24(), 1953

Kakorin, J. Phys. Chem. B 107(), 2003

Kakorin, Ber. Bunsen-Ges. Phys. Chem. 102(), 1998
Ionic conductivity of electroporated lipid bilayer membranes.
Kakorin S, Neumann E., Bioelectrochemistry 56(1-2), 2002
PMID: 12009466
Atomistic simulations of bicelle mixtures.
Jiang Y, Wang H, Kindt JT., Biophys. J. 98(12), 2010
PMID: 20550902
Asymmetric pore distribution and loss of membrane lipid in electroporated DOPC vesicles.
Tekle E, Astumian RD, Friauf WA, Chock PB., Biophys. J. 81(2), 2001
PMID: 11463638
High electrical field effects on cell membranes.
Pliquett U, Joshi RP, Sridhara V, Schoenbach KH., Bioelectrochemistry 70(2), 2007
PMID: 17123870

Tamura, Adv. Mol. Relax. Interact. Processes 24(), 1982
The dielectric properties of water within model transbilayer pores.
Sansom MS, Smith GR, Adcock C, Biggin PC., Biophys. J. 73(5), 1997
PMID: 9370434
Membrane dipole potentials, hydration forces, and the ordering of water at membrane surfaces.
Gawrisch K, Ruston D, Zimmerberg J, Parsegian VA, Rand RP, Fuller N., Biophys. J. 61(5), 1992
PMID: 1600081

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