Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons

Klenke C, Widera D, Engelen T, Müller J, Noll T, Niehaus K, Schmitz M, Kaltschmidt B, Kaltschmidt C (2013)
PLoS ONE 8(6).

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Abstract
Signaling via NF-κB in neurons depends on complex formation with interactors such as dynein/dynactin motor complex and can be triggered by synaptic activation. However, so far a detailed interaction map for the neuronal NF-κB is missing. In this study we used mass spectrometry to identify novel interactors of NF-κB p65 within the brain. Hsc70 was identified as a novel neuronal interactor of NF-κB p65. In HEK293 cells, a direct physical interaction was shown by co-immunoprecipitation and verified via in situ proximity ligation in healthy rat neurons. Pharmacological blockade of Hsc70 by deoxyspergualin (DSG) strongly decreased nuclear translocation of NF-κB p65 and transcriptional activity shown by reporter gene assays in neurons after stimulation with glutamate. In addition, knock down of Hsc70 via siRNA significantly reduced neuronal NF-κB activity. Taken together these data provide evidence for Hsc70 as a novel neuronal interactor of NF-κB p65.
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Klenke C, Widera D, Engelen T, et al. Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE. 2013;8(6).
Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., et al. (2013). Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE, 8(6).
Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., Kaltschmidt, B., and Kaltschmidt, C. (2013). Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE 8.
Klenke, C., et al., 2013. Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE, 8(6).
C. Klenke, et al., “Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons”, PLoS ONE, vol. 8, 2013.
Klenke, C., Widera, D., Engelen, T., Müller, J., Noll, T., Niehaus, K., Schmitz, M., Kaltschmidt, B., Kaltschmidt, C.: Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons. PLoS ONE. 8, (2013).
Klenke, Christin, Widera, Darius, Engelen, Thomas, Müller, Janine, Noll, Thomas, Niehaus, Karsten, Schmitz, M., Kaltschmidt, Barbara, and Kaltschmidt, Christian. “Hsc70 is a novel interactor of NF-kappaB p65 in living hippocampal neurons”. PLoS ONE 8.6 (2013).
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