The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins

Barabino SM, Hübner W, Jenny A, Minvielle-Sebastia L, Keller W (1997)
Genes & development 11(13): 1703-1716.

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Abstract
Cleavage and polyadenylation specificity factor (CPSF), a key component of the mammalian RNA 3'-end processing machinery, consists of four subunits of 160, 100, 73, and 30 kD. Here we report the isolation and characterization of a cDNA encoding the 30-kD polypeptide. Antibodies raised against this protein inhibit cleavage and polyadenylation and coimmunoprecipitate the other CPSF subunits. The protein sequence contains five C3H-zinc-finger repeats and a putative RNA-binding zinc knuckle motif at the carboxyl terminus. Consistent with this observation, the in vitro translated 30-kD protein binds RNA polymers with a distinct preference for poly(U). In addition, an essential S. cerevisiae gene, YTH1, was cloned which is 40% identical to CPSF 30K at the protein level. Extracts prepared from a conditional yth1 mutant have normal cleavage activity, but fail to polyadenylate the upstream cleavage product. Efficient polyadenylation activity can be restored by the addition of purified polyadenylation factor I (PF I). We demonstrate that Yth1p is a component of PF I that interacts in vivo and in vitro with Fip1p, a known PF I subunit.
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Barabino SM, Hübner W, Jenny A, Minvielle-Sebastia L, Keller W. The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & development. 1997;11(13):1703-1716.
Barabino, S. M., Hübner, W., Jenny, A., Minvielle-Sebastia, L., & Keller, W. (1997). The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & development, 11(13), 1703-1716.
Barabino, S. M., Hübner, W., Jenny, A., Minvielle-Sebastia, L., and Keller, W. (1997). The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & development 11, 1703-1716.
Barabino, S.M., et al., 1997. The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & development, 11(13), p 1703-1716.
S.M. Barabino, et al., “The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins”, Genes & development, vol. 11, 1997, pp. 1703-1716.
Barabino, S.M., Hübner, W., Jenny, A., Minvielle-Sebastia, L., Keller, W.: The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins. Genes & development. 11, 1703-1716 (1997).
Barabino, S M, Hübner, Wolfgang, Jenny, A, Minvielle-Sebastia, L, and Keller, W. “The 30-kD subunit of mammalian cleavage and polyadenylation specificity factor and its yeast homolog are RNA-binding zinc finger proteins”. Genes & development 11.13 (1997): 1703-1716.
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