Quantitative 3D video microscopy of HIV transfer across T cell virological synapses

Hübner W, McNerney GP, Chen P, Dale BM, Gordon RE, Chuang FYS, Li X-D, Asmuth DM, Huser T, Chen BK (2009)
Science (New York, N.Y.) 323(5922): 1743-1747.

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The spread of HIV between immune cells is greatly enhanced by cell-cell adhesions called virological synapses, although the underlying mechanisms have been unclear. With use of an infectious, fluorescent clone of HIV, we tracked the movement of Gag in live CD4 T cells and captured the direct translocation of HIV across the virological synapse. Quantitative, high-speed three-dimensional (3D) video microscopy revealed the rapid formation of micrometer-sized "buttons" containing oligomerized viral Gag protein. Electron microscopy showed that these buttons were packed with budding viral crescents. Viral transfer events were observed to form virus-laden internal compartments within target cells. Continuous time-lapse monitoring showed preferential infection through synapses. Thus, HIV dissemination may be enhanced by virological synapse-mediated cell adhesion coupled to viral endocytosis.
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Hübner W, McNerney GP, Chen P, et al. Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science (New York, N.Y.). 2009;323(5922):1743-1747.
Hübner, W., McNerney, G. P., Chen, P., Dale, B. M., Gordon, R. E., Chuang, F. Y. S., Li, X. - D., et al. (2009). Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science (New York, N.Y.), 323(5922), 1743-1747. doi:10.1126/science.1167525
Hübner, W., McNerney, G. P., Chen, P., Dale, B. M., Gordon, R. E., Chuang, F. Y. S., Li, X. - D., Asmuth, D. M., Huser, T., and Chen, B. K. (2009). Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science (New York, N.Y.) 323, 1743-1747.
Hübner, W., et al., 2009. Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science (New York, N.Y.), 323(5922), p 1743-1747.
W. Hübner, et al., “Quantitative 3D video microscopy of HIV transfer across T cell virological synapses”, Science (New York, N.Y.), vol. 323, 2009, pp. 1743-1747.
Hübner, W., McNerney, G.P., Chen, P., Dale, B.M., Gordon, R.E., Chuang, F.Y.S., Li, X.-D., Asmuth, D.M., Huser, T., Chen, B.K.: Quantitative 3D video microscopy of HIV transfer across T cell virological synapses. Science (New York, N.Y.). 323, 1743-1747 (2009).
Hübner, Wolfgang, McNerney, Gregory P, Chen, Ping, Dale, Benjamin M, Gordon, Ronald E, Chuang, Frank Y S, Li, Xiao-Dong, Asmuth, David M, Huser, Thomas, and Chen, Benjamin K. “Quantitative 3D video microscopy of HIV transfer across T cell virological synapses”. Science (New York, N.Y.) 323.5922 (2009): 1743-1747.
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26 References

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Dynamic imaging of the immune system: progress, pitfalls and promise.
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