Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.

Sporbert A, Cseresnyes Z, Heidbreder M, Domaing P, Hauser S, Kaltschmidt B, Kaltschmidt C, Heilemann M, Widera D (2013)
PLoS ONE 8(5).

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This study describes a simple technique that improves a recently developed 3D sub-diffraction imaging method based on three-photon absorption of commercially available quantum dots. The method combines imaging of biological samples via tri-exciton generation in quantum dots with deconvolution and spectral multiplexing, resulting in a novel approach for multi-color imaging of even thick biological samples at a 1.4 to 1.9-fold better spatial resolution. This approach is realized on a conventional confocal microscope equipped with standard continuous-wave lasers. We demonstrate the potential of multi-color tri-exciton imaging of quantum dots combined with deconvolution on viral vesicles in lentivirally transduced cells as well as intermediate filaments in three-dimensional clusters of mouse-derived neural stem cells (neurospheres) and dense microtubuli arrays in myotubes formed by stacks of differentiated C2C12 myoblasts.
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Sporbert A, Cseresnyes Z, Heidbreder M, et al. Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE. 2013;8(5).
Sporbert, A., Cseresnyes, Z., Heidbreder, M., Domaing, P., Hauser, S., Kaltschmidt, B., Kaltschmidt, C., et al. (2013). Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE, 8(5).
Sporbert, A., Cseresnyes, Z., Heidbreder, M., Domaing, P., Hauser, S., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M., and Widera, D. (2013). Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE 8.
Sporbert, A., et al., 2013. Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE, 8(5).
A. Sporbert, et al., “Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.”, PLoS ONE, vol. 8, 2013.
Sporbert, A., Cseresnyes, Z., Heidbreder, M., Domaing, P., Hauser, S., Kaltschmidt, B., Kaltschmidt, C., Heilemann, M., Widera, D.: Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots. PLoS ONE. 8, (2013).
Sporbert, Anje, Cseresnyes, Zoltan, Heidbreder, Meike, Domaing, Petra, Hauser, Stefan, Kaltschmidt, Barbara, Kaltschmidt, Christian, Heilemann, Mike, and Widera, Darius. “Simple method for sub-diffraction resolution imaging of cellular structures on standard confocal microscopes by three-photon absorption of quantum dots.”. PLoS ONE 8.5 (2013).
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