Elements of transcriptional machinery are compatible among plants and mammals

Wolf A, Akrap N, Marg B, Galliardt H, Heiligentag M, Humpert F, Sauer M, Kaltschmidt B, Kaltschmidt C, Seidel T (2013)
PLoS ONE 8(1).

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Abstract
In the present work, the objective has been to analyse the compatibility of plant and human transcriptional machinery. The experiments revealed that nuclear import and export are conserved among plants and mammals. Further it has been shown that transactivation of a human promoter occurs by human transcription factor NF-κB in plant cells, demonstrating that the transcriptional machinery is highly conserved in both kingdoms. Functionality was also seen for regulatory elements of NF-κB such as its inhibitor IκB isoform α that negatively regulated the transactivation activity of the p50/RelA heterodimer by interaction with NF-κB in plant cells. Nuclear export of RelA could be demonstrated by FRAP-measurements so that RelA shows nucleo-cytoplasmic shuttling as reported for RelA in mammalian cells. The data reveals the high level of compatibility of human transcriptional elements with the plant transcriptional machinery. Thus, Arabidopsis thaliana mesophyll protoplasts might provide a new heterologous expression system for the investigation of the human NF-κB signaling pathways. The system successfully enabled the controlled manipulation of NF-κB activity. We suggest the plant protoplast system as a tool for reconstitution and analyses of mammalian pathways and for direct observation of responses to e.g. pharmaceuticals. The major advantage of the system is the absence of interference with endogenous factors that affect and crosstalk with the pathway.
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Wolf A, Akrap N, Marg B, et al. Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE. 2013;8(1).
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., et al. (2013). Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE, 8(1).
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., Kaltschmidt, B., Kaltschmidt, C., and Seidel, T. (2013). Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE 8.
Wolf, A., et al., 2013. Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE, 8(1).
A. Wolf, et al., “Elements of transcriptional machinery are compatible among plants and mammals”, PLoS ONE, vol. 8, 2013.
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., Kaltschmidt, B., Kaltschmidt, C., Seidel, T.: Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE. 8, (2013).
Wolf, Annette, Akrap, Nina, Marg, Berenice, Galliardt, Helena, Heiligentag, Martyna, Humpert, Fabian, Sauer, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Seidel, Thorsten. “Elements of transcriptional machinery are compatible among plants and mammals”. PLoS ONE 8.1 (2013).
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Greiner JF, Muller J, Zeuner MT, Hauser S, Seidel T, Klenke C, Grunwald LM, Schomann T, Widera D, Sudhoff H, Kaltschmidt B, Kaltschmidt C., Biochim. Biophys. Acta 1833(12), 2013
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31 References

Data provided by Europe PubMed Central.

Arabidopsis transportin1 is the nuclear import receptor for the circadian clock-regulated RNA-binding protein AtGRP7.
Ziemienowicz A, Haasen D, Staiger D, Merkle T., Plant Mol. Biol. 53(1-2), 2003
PMID: 14756317
Sensitive detection of p65 homodimers using red-shifted and fluorescent protein-based FRET couples.
Goedhart J, Vermeer JE, Adjobo-Hermans MJ, van Weeren L, Gadella TW Jr., PLoS ONE 2(10), 2007
PMID: 17925859
Transcriptional regulation via the NF-kappaB signaling module.
Hoffmann A, Natoli G, Ghosh G., Oncogene 25(51), 2006
PMID: 17072323

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