Elements of transcriptional machinery are compatible among plants and mammals

Wolf A, Akrap N, Marg B, Galliardt H, Heiligentag M, Humpert F, Sauer M, Kaltschmidt B, Kaltschmidt C, Seidel T (2013)
PLoS ONE 8(1): e53737.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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In the present work, the objective has been to analyse the compatibility of plant and human transcriptional machinery. The experiments revealed that nuclear import and export are conserved among plants and mammals. Further it has been shown that transactivation of a human promoter occurs by human transcription factor NF-κB in plant cells, demonstrating that the transcriptional machinery is highly conserved in both kingdoms. Functionality was also seen for regulatory elements of NF-κB such as its inhibitor IκB isoform α that negatively regulated the transactivation activity of the p50/RelA heterodimer by interaction with NF-κB in plant cells. Nuclear export of RelA could be demonstrated by FRAP-measurements so that RelA shows nucleo-cytoplasmic shuttling as reported for RelA in mammalian cells. The data reveals the high level of compatibility of human transcriptional elements with the plant transcriptional machinery. Thus, Arabidopsis thaliana mesophyll protoplasts might provide a new heterologous expression system for the investigation of the human NF-κB signaling pathways. The system successfully enabled the controlled manipulation of NF-κB activity. We suggest the plant protoplast system as a tool for reconstitution and analyses of mammalian pathways and for direct observation of responses to e.g. pharmaceuticals. The major advantage of the system is the absence of interference with endogenous factors that affect and crosstalk with the pathway.
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PLoS ONE
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8
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1
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e53737
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Wolf A, Akrap N, Marg B, et al. Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE. 2013;8(1):e53737.
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., et al. (2013). Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE, 8(1), e53737. doi:10.1371/journal.pone.0053737
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., Kaltschmidt, B., Kaltschmidt, C., and Seidel, T. (2013). Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE 8, e53737.
Wolf, A., et al., 2013. Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE, 8(1), p e53737.
A. Wolf, et al., “Elements of transcriptional machinery are compatible among plants and mammals”, PLoS ONE, vol. 8, 2013, pp. e53737.
Wolf, A., Akrap, N., Marg, B., Galliardt, H., Heiligentag, M., Humpert, F., Sauer, M., Kaltschmidt, B., Kaltschmidt, C., Seidel, T.: Elements of transcriptional machinery are compatible among plants and mammals. PLoS ONE. 8, e53737 (2013).
Wolf, Annette, Akrap, Nina, Marg, Berenice, Galliardt, Helena, Heiligentag, Martyna, Humpert, Fabian, Sauer, Markus, Kaltschmidt, Barbara, Kaltschmidt, Christian, and Seidel, Thorsten. “Elements of transcriptional machinery are compatible among plants and mammals”. PLoS ONE 8.1 (2013): e53737.
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2017-11-07T10:16:44Z

3 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

A disulphide-linked heterodimer of TWIK-1 and TREK-1 mediates passive conductance in astrocytes.
Hwang EM, Kim E, Yarishkin O, Woo DH, Han KS, Park N, Bae Y, Woo J, Kim D, Park M, Lee CJ, Park JY., Nat Commun 5(), 2014
PMID: 24496152
1,8-Cineol inhibits nuclear translocation of NF-κB p65 and NF-κB-dependent transcriptional activity.
Greiner JF, Müller J, Zeuner MT, Hauser S, Seidel T, Klenke C, Grunwald LM, Schomann T, Widera D, Sudhoff H, Kaltschmidt B, Kaltschmidt C., Biochim Biophys Acta 1833(12), 2013
PMID: 23872422
Quantification of Förster resonance energy transfer by monitoring sensitized emission in living plant cells.
Müller SM, Galliardt H, Schneider J, Barisas BG, Seidel T., Front Plant Sci 4(), 2013
PMID: 24194740

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