Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411

Barzantny H, Guttmann S, Lässig C, Brune I, Tauch A (2013)
Microbial Biotechnology 6(2): 118-130.

Download
No fulltext has been uploaded. References only!
Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Corynebacterial fatty acid metabolism has been associated with human body odour, and is therefore discussed as a potential target for the development of new deodorant additives. For this reason, the transcription levels of fad genes associated with lipid metabolism in the axilla isolate Corynebacterium jeikeium were analysed during growth on different lipid sources. The transcription of several fad genes was induced two- to ninefold in the presence of Tween 60, including the acyl-CoA dehydrogenase gene fadE6. DNA affinity chromatography identified the MarR-like protein FamR as candidate regulator of fadE6. DNA band shift assays and in vivo reporter gene fusions confirmed the direct interaction of FamR with the mapped fadE6 promoter region. Moreover, DNA affinity chromatography and DNA band shift assays detected the binding of GlxR to the promoter regions of fadE6 and famR, revealing a hierarchical control of fadE6 transcription by a feed-forward loop. Binding of GlxR and FamR to additional fad gene regions was demonstrated in vitro by DNA band shift assays, resulting in the co-regulation of fadA, fadD, fadE and fadH genes. These results shed first light on the hierarchical transcriptional control of lipid metabolism in C.jeikeium, a pathway associated with the development of human axillary odour. (2012 The Authors. Microbial Biotechnology 2012 Society for Applied Microbiology and Blackwell Publishing Ltd.)
Publishing Year
ISSN
PUB-ID

Cite this

Barzantny H, Guttmann S, Lässig C, Brune I, Tauch A. Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411. Microbial Biotechnology. 2013;6(2):118-130.
Barzantny, H., Guttmann, S., Lässig, C., Brune, I., & Tauch, A. (2013). Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411. Microbial Biotechnology, 6(2), 118-130. doi:10.1111/1751-7915.12004
Barzantny, H., Guttmann, S., Lässig, C., Brune, I., and Tauch, A. (2013). Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411. Microbial Biotechnology 6, 118-130.
Barzantny, H., et al., 2013. Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411. Microbial Biotechnology, 6(2), p 118-130.
H. Barzantny, et al., “Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411”, Microbial Biotechnology, vol. 6, 2013, pp. 118-130.
Barzantny, H., Guttmann, S., Lässig, C., Brune, I., Tauch, A.: Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411. Microbial Biotechnology. 6, 118-130 (2013).
Barzantny, Helena, Guttmann, Sarah, Lässig, Charlotte, Brune, Iris, and Tauch, Andreas. “Transcriptional control of lipid metabolism by the MarR-like regulator FamR and the global regulator GlxR in the lipophilic axilla isolateCorynebacterium jeikeiumK411”. Microbial Biotechnology 6.2 (2013): 118-130.
This data publication is cited in the following publications:
This publication cites the following data publications:

41 References

Data provided by Europe PubMed Central.

Cutaneous manifestations of Corynebacterium jeikeium sepsis.
Olson JM, Nguyen VQ, Yoo J, Kuechle MK., Int. J. Dermatol. 48(8), 2009
PMID: 19659870
Epidermal surface lipids.
Pappas A., Dermatoendocrinol 1(2), 2009
PMID: 20224687
ChIP-seq: advantages and challenges of a maturing technology.
Park PJ., Nat. Rev. Genet. 10(10), 2009
PMID: 19736561
Transcriptional repression: conserved and evolved features.
Payankaulam S, Li LM, Arnosti DN., Curr. Biol. 20(17), 2010
PMID: 20833321
MarR homologs with urate-binding signature.
Perera IC, Grove A., Protein Sci. 20(3), 2011
PMID: 21432936

Sambrook R, Russel DW., 2001
Corynebacterium glutamicum DNA is subjected to methylation-restriction in Escherichia coli.
Tauch A, Kirchner O, Wehmeier L, Kalinowski J, Puhler A., FEMS Microbiol. Lett. 123(3), 1994
PMID: 7988915
Efficient electrotransformation of corynebacterium diphtheriae with a mini-replicon derived from the Corynebacterium glutamicum plasmid pGA1.
Tauch A, Kirchner O, Loffler B, Gotker S, Puhler A, Kalinowski J., Curr. Microbiol. 45(5), 2002
PMID: 12232668
Complete genome sequence and analysis of the multiresistant nosocomial pathogen Corynebacterium jeikeium K411, a lipid-requiring bacterium of the human skin flora.
Tauch A, Kaiser O, Hain T, Goesmann A, Weisshaar B, Albersmeier A, Bekel T, Bischoff N, Brune I, Chakraborty T, Kalinowski J, Meyer F, Rupp O, Schneiker S, Viehoever P, Puhler A., J. Bacteriol. 187(13), 2005
PMID: 15968079
Characterization of the microflora of the human axilla.
Taylor D, Daulby A, Grimshaw S, James G, Mercer J, Vaziri S., Int J Cosmet Sci 25(3), 2003
PMID: 18494895
Composition analysis of two batches of polysorbate 60 using MS and NMR techniques.
Vu Dang H, Gray AI, Watson D, Bates CD, Scholes P, Eccleston GM., J Pharm Biomed Anal 40(5), 2006
PMID: 16280224

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 23163914
PubMed | Europe PMC

Search this title in

Google Scholar