Video-based and interference-free axial force detection and analysis for optical tweezers

Knust S, Spiering A, Vieker H, Beyer A, Gölzhäuser A, Tönsing K, Sischka A, Anselmetti D (2012)
Review Of Scientific Instruments 83(10).

Journal Article | Published | English

No fulltext has been uploaded

Abstract
For measuring the minute forces exerted on single molecules during controlled translocation through nanopores with sub-piconewton precision, we have developed a video-based axial force detection and analysis system for optical tweezers. Since our detection system is equipped with a standard and versatile CCD video camera with a limited bandwidth offering operation at moderate light illumination with minimal sample heating, we integrated Allan variance analysis for trap stiffness calibration. Upon manipulating a microbead in the vicinity of a weakly reflecting surface with simultaneous axial force detection, interference effects have to be considered and minimized. We measured and analyzed the backscattering light properties of polystyrene and silica microbeads with different diameters and propose distinct and optimized experimental configurations (microbead material and diameter) for minimal light backscattering and virtually interference-free microbead position detection. As a proof of principle, we investigated the nanopore threading forces of a single dsDNA strand attached to a microbead with an overall force resolution of +/- 0.5 pN at a sample rate of 123 Hz. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.4757397]
Publishing Year
ISSN
PUB-ID

Cite this

Knust S, Spiering A, Vieker H, et al. Video-based and interference-free axial force detection and analysis for optical tweezers. Review Of Scientific Instruments. 2012;83(10).
Knust, S., Spiering, A., Vieker, H., Beyer, A., Gölzhäuser, A., Tönsing, K., Sischka, A., et al. (2012). Video-based and interference-free axial force detection and analysis for optical tweezers. Review Of Scientific Instruments, 83(10).
Knust, S., Spiering, A., Vieker, H., Beyer, A., Gölzhäuser, A., Tönsing, K., Sischka, A., and Anselmetti, D. (2012). Video-based and interference-free axial force detection and analysis for optical tweezers. Review Of Scientific Instruments 83.
Knust, S., et al., 2012. Video-based and interference-free axial force detection and analysis for optical tweezers. Review Of Scientific Instruments, 83(10).
S. Knust, et al., “Video-based and interference-free axial force detection and analysis for optical tweezers”, Review Of Scientific Instruments, vol. 83, 2012.
Knust, S., Spiering, A., Vieker, H., Beyer, A., Gölzhäuser, A., Tönsing, K., Sischka, A., Anselmetti, D.: Video-based and interference-free axial force detection and analysis for optical tweezers. Review Of Scientific Instruments. 83, (2012).
Knust, Sebastian, Spiering, André, Vieker, Henning, Beyer, André, Gölzhäuser, Armin, Tönsing, Katja, Sischka, Andy, and Anselmetti, Dario. “Video-based and interference-free axial force detection and analysis for optical tweezers”. Review Of Scientific Instruments 83.10 (2012).
This data publication is cited in the following publications:
This publication cites the following data publications:

2 Citations in Europe PMC

Data provided by Europe PubMed Central.

Controlled translocation of DNA through nanopores in carbon nano-, silicon-nitride- and lipid-coated membranes.
Sischka A, Galla L, Meyer AJ, Spiering A, Knust S, Mayer M, Hall AR, Beyer A, Reimann P, Golzhauser A, Anselmetti D., Analyst 140(14), 2015
PMID: 25768647
A high-speed vertical optical trap for the mechanical testing of living cells at piconewton forces.
Bodensiek K, Li W, Sanchez P, Nawaz S, Schaap IA., Rev Sci Instrum 84(11), 2013
PMID: 24289404

20 References

Data provided by Europe PubMed Central.

Recent advances in optical tweezers.
Moffitt JR, Chemla YR, Smith SB, Bustamante C., Annu. Rev. Biochem. 77(), 2008
PMID: 18307407

AUTHOR UNKNOWN, 0

AUTHOR UNKNOWN, 0
Force detection in optical tweezers using backscattered light.
Huisstede J, van der Werf K, Bennink M, Subramaniam V., Opt Express 13(4), 2005
PMID: 19494979

AUTHOR UNKNOWN, 0
Single beam optical tweezers setup with backscattered light detection for three-dimensional measurements on DNA and nanopores.
Sischka A, Kleimann C, Hachmann W, Schafer MM, Seuffert I, Tonsing K, Anselmetti D., Rev Sci Instrum 79(6), 2008
PMID: 18601408
An automated two-dimensional optical force clamp for single molecule studies.
Lang MJ, Asbury CL, Shaevitz JW, Block SM., Biophys. J. 83(1), 2002
PMID: 12080136
Real-time particle tracking at 10,000 fps using optical fiber illumination.
Otto O, Czerwinski F, Gornall JL, Stober G, Oddershede LB, Seidel R, Keyser UF., Opt Express 18(22), 2010
PMID: 21164611
Dynamic translocation of ligand-complexed DNA through solid-state nanopores with optical tweezers.
Sischka A, Spiering A, Khaksar M, Laxa M, Konig J, Dietz KJ, Anselmetti D., J Phys Condens Matter 22(45), 2010
PMID: 21339608

AUTHOR UNKNOWN, 0
Optical tweezers with 2.5 kHz bandwidth video detection for single-colloid electrophoresis.
Otto O, Gutsche C, Kremer F, Keyser UF., Rev Sci Instrum 79(2 Pt 1), 2008
PMID: 18315308
Measuring the accuracy of particle position and force in optical tweezers using high-speed video microscopy.
Gibson GM, Leach J, Keen S, Wright AJ, Padgett MJ., Opt Express 16(19), 2008
PMID: 18794991
Single-beam trapping in front of reflective surfaces.
Jonass A, Zemanek P, Florin EL., Opt Lett 26(19), 2001
PMID: 18049636
Optical trapping of coated microspheres.
Bormuth V, Jannasch A, Ander M, van Kats CM, van Blaaderen A, Howard J, Schaffer E., Opt Express 16(18), 2008
PMID: 18772994
Nanopore translocation dynamics of a single DNA-bound protein.
Spiering A, Getfert S, Sischka A, Reimann P, Anselmetti D., Nano Lett. 11(7), 2011
PMID: 21667921

AUTHOR UNKNOWN, 0
Direct force measurements on double-stranded RNA in solid-state nanopores.
van den Hout M, Vilfan ID, Hage S, Dekker NH., Nano Lett. 10(2), 2010
PMID: 20050676

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 23126771
PubMed | Europe PMC

Search this title in

Google Scholar