The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression

Klein P, Seidel T, Stöcker B, Dietz K-J (2012)
Frontiers in Plant Science 3: 247.

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Abstract / Bemerkung
The stromal ascorbate peroxidase (sAPX) functions as central element of the chloroplast antioxidant defense system. Its expression is under retrograde control of chloroplast signals including redox- and reactive oxygen species-linked cues. The sAPX promoter of Arabidopsis thaliana was dissected in transient reporter assays using mesophyll protoplasts. The study revealed regulatory elements up to -1868 upstream of the start codon. By yeast-one-hybrid screening, the transcription factor ANAC089 was identified to bind to the promoter fragment 2 (-1262 to -1646 bp upstream of translational initiation). Upon mutation of the cis-acting element CACG, binding of ANAC089 was abolished. Expression of a fused fluorescent protein version and comparison with known endomembrane markers localized ANAC089 to the trans-Golgi network and the ER. The transcription factor was released upon treatment with reducing agents and targeted to the nucleus. Transactivation assays using wild type and mutated versions of the promoter showed a partial suppression of reporter expression. The data indicate that ANAC089 functions in a negative retrograde loop, lowering sAPX expression if the cell encounters a highly reducing condition. This conclusion was supported by reciprocal transcript accumulation of ANAC089 and sAPX during acclimation to low, normal, and high light conditions.
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Frontiers in Plant Science
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3
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247
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Article Processing Charge funded by the Deutsche Forschungsgemeinschaft and the Open Access Publication Fund of Bielefeld University.
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Klein P, Seidel T, Stöcker B, Dietz K-J. The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression. Frontiers in Plant Science. 2012;3:247.
Klein, P., Seidel, T., Stöcker, B., & Dietz, K. - J. (2012). The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression. Frontiers in Plant Science, 3, 247. doi:10.3389/fpls.2012.00247
Klein, P., Seidel, T., Stöcker, B., and Dietz, K. - J. (2012). The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression. Frontiers in Plant Science 3, 247.
Klein, P., et al., 2012. The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression. Frontiers in Plant Science, 3, p 247.
P. Klein, et al., “The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression”, Frontiers in Plant Science, vol. 3, 2012, pp. 247.
Klein, P., Seidel, T., Stöcker, B., Dietz, K.-J.: The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression. Frontiers in Plant Science. 3, 247 (2012).
Klein, Peter, Seidel, Thorsten, Stöcker, Benedikt, and Dietz, Karl-Josef. “The membrane-tethered transcription factor ANAC089 serves as redox-dependent suppressor of stromal ascorbate peroxidase gene expression”. Frontiers in Plant Science 3 (2012): 247.
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2012-12-05T14:19:03Z

11 Zitationen in Europe PMC

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Subcellular Distribution of NTL Transcription Factors in Arabidopsis thaliana.
Liang M, Li H, Zhou F, Li H, Liu J, Hao Y, Wang Y, Zhao H, Han S., Traffic 16(10), 2015
PMID: 26201836
The ABI4-induced Arabidopsis ANAC060 transcription factor attenuates ABA signaling and renders seedlings sugar insensitive when present in the nucleus.
Li P, Zhou H, Shi X, Yu B, Zhou Y, Chen S, Wang Y, Peng Y, Meyer RC, Smeekens SC, Teng S., PLoS Genet 10(3), 2014
PMID: 24625790
The membrane-associated transcription factor NAC089 controls ER-stress-induced programmed cell death in plants.
Yang ZT, Wang MJ, Sun L, Lu SJ, Bi DL, Sun L, Song ZT, Zhang SS, Zhou SF, Liu JX., PLoS Genet 10(3), 2014
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Identification of target genes and transcription factors implicated in translation-dependent retrograde signaling in Arabidopsis.
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13 References

Daten bereitgestellt von Europe PubMed Central.

Protease gene families in Populus and Arabidopsis.
Garcia-Lorenzo M, Sjodin A, Jansson S, Funk C., BMC Plant Biol. 6(), 2006
PMID: 17181860
Comprehensive analysis of NAC domain transcription factor gene family in Populus trichocarpa.
Hu R, Qi G, Kong Y, Kong D, Gao Q, Zhou G., BMC Plant Biol. 10(), 2010
PMID: 20630103
Exploring membrane-associated NAC transcription factors in Arabidopsis: implications for membrane biology in genome regulation.
Kim SY, Kim SG, Kim YS, Seo PJ, Bae M, Yoon HK, Park CM., Nucleic Acids Res. 35(1), 2007
PMID: 17158162
Fructose sensitivity is suppressed in Arabidopsis by the transcription factor ANAC089 lacking the membrane-bound domain.
Li P, Wind JJ, Shi X, Zhang H, Hanson J, Smeekens SC, Teng S., Proc. Natl. Acad. Sci. U.S.A. 108(8), 2011
PMID: 21300879
AFP is a novel negative regulator of ABA signaling that promotes ABI5 protein degradation.
Lopez-Molina L, Mongrand S, Kinoshita N, Chua NH., Genes Dev. 17(3), 2003
PMID: 12569131
Arabidopsis has two functional orthologs of the yeast V-ATPase assembly factor Vma21p.
Neubert C, Graham LA, Black-Maier EW, Coonrod EM, Liu TY, Stierhof YD, Seidel T, Stevens TH, Schumacher K., Traffic 9(10), 2008
PMID: 18694437
Organelle-specific isoenzymes of plant V-ATPase as revealed by in vivo-FRET analysis.
Seidel T, Schnitzer D, Golldack D, Sauer M, Dietz KJ., BMC Cell Biol. 9(), 2008
PMID: 18507826
The redox-sensitive transcription factor Rap2.4a controls nuclear expression of 2-Cys peroxiredoxin A and other chloroplast antioxidant enzymes.
Shaikhali J, Heiber I, Seidel T, Stroher E, Hiltscher H, Birkmann S, Dietz KJ, Baier M., BMC Plant Biol. 8(), 2008
PMID: 18439303
Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability.
Wormuth D, Baier M, Kandlbinder A, Scheibe R, Hartung W, Dietz KJ., BMC Plant Biol. 6(), 2006
PMID: 16916444
Peroxiredoxins in plants and cyanobacteria.
Dietz KJ., Antioxid. Redox Signal. 15(4), 2011
PMID: 21194355
Structure of the conserved domain of ANAC, a member of the NAC family of transcription factors.
Ernst HA, Olsen AN, Larsen S, Lo Leggio L., EMBO Rep. 5(3), 2004
PMID: 15083810

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