Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities

Schwekendiek A, Spring O, Heyerick A, Pickel B, Pitsch NT, Peschke F, de Keukeleire D, Weber G (2007)
Journal of agricultural and food chemistry 55(17): 7002-7009.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Resveratrol, a well-known phytoalexin and antioxidant, is produced by the action of stilbene synthase (STS) in some plant species. Hop (Humulus lupulus L.) plants of the Tettnang variety were transformed with a gene encoding for STS from grapevine. Under the control of the constitutive 35S cauliflower mosaic virus promoter, expression of the transgene resulted in accumulation of resveratrol and high levels of its glycosylated derivatives in leaves and inflorescences. Piceid, the predominant derivative, reached a concentration of up to 560 microg/g of fresh weight (f.w.) in hop cones, whereas no stilbenes were detected in nontransformed controls (wild-type). In transgenic plants the amounts of alpha- and beta-acids, naringenin chalcone, and prenylated flavonoids did not change significantly when compared with nontransformed plants. Transgenic plants showed normal morphology and flower development as did the nontransformed controls. The results clearly show that in hop constitutive expression of sts interferes neither with plant development nor with the biosynthesis of secondary metabolites relevant for the brewing industry. Since resveratrol is a well-known phytoalexin and antioxidant, sts transgenic hop plants could display enhanced pathogen resistance against microbial pathogens, exhibit new beneficial properties for health, and open new venues for metabolic engineering.
Erscheinungsjahr
Zeitschriftentitel
Journal of agricultural and food chemistry
Band
55
Zeitschriftennummer
17
Seite
7002-7009
ISSN
eISSN
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Schwekendiek A, Spring O, Heyerick A, et al. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 2007;55(17):7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., et al. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), 7002-7009. doi:10.1021/jf070509e
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., and Weber, G. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry 55, 7002-7009.
Schwekendiek, A., et al., 2007. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), p 7002-7009.
A. Schwekendiek, et al., “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”, Journal of agricultural and food chemistry, vol. 55, 2007, pp. 7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N.T., Peschke, F., de Keukeleire, D., Weber, G.: Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 55, 7002-7009 (2007).
Schwekendiek, Axel, Spring, Otmar, Heyerick, Arne, Pickel, Benjamin, Pitsch, Nicola T, Peschke, Florian, de Keukeleire, Denis, and Weber, Gerd. “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”. Journal of agricultural and food chemistry 55.17 (2007): 7002-7009.

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Tyunin AP, Nityagovsky NN, Grigorchuk VP, Kiselev KV., Biotechnol Appl Biochem 65(2), 2018
PMID: 28332216
Natural products against Alzheimer's disease: Pharmaco-therapeutics and biotechnological interventions.
Dey A, Bhattacharya R, Mukherjee A, Pandey DK., Biotechnol Adv 35(2), 2017
PMID: 28043897
Characterization of NtREL1, a novel root-specific gene from tobacco, and upstream promoter activity analysis in homologous and heterologous hosts.
Zhang C, Pan S, Chen H, Cai T, Zhuang C, Deng Y, Zhuang Y, Zeng Y, Chen S, Zhuang W., Plant Cell Rep 35(4), 2016
PMID: 26849672
Evaluating the Effect of Expressing a Peanut Resveratrol Synthase Gene in Rice.
Zheng S, Zhao S, Li Z, Wang Q, Yao F, Yang L, Pan J, Liu W., PLoS One 10(8), 2015
PMID: 26302213
Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato.
Pan LP, Yu SL, Chen CJ, Li H, Wu YL, Li HH., Plant Cell Rep 31(1), 2012
PMID: 21932029
Phytoalexins in defense against pathogens.
Ahuja I, Kissen R, Bones AM., Trends Plant Sci 17(2), 2012
PMID: 22209038
Metabolic engineering of yeast and plants for the production of the biologically active hydroxystilbene, resveratrol.
Jeandet P, Delaunois B, Aziz A, Donnez D, Vasserot Y, Cordelier S, Courot E., J Biomed Biotechnol 2012(), 2012
PMID: 22654481
Resveratrol biosynthesis: plant metabolic engineering for nutritional improvement of food.
Giovinazzo G, Ingrosso I, Paradiso A, De Gara L, Santino A., Plant Foods Hum Nutr 67(3), 2012
PMID: 22777386
Water deficit increases stilbene metabolism in Cabernet Sauvignon berries.
Deluc LG, Decendit A, Papastamoulis Y, Mérillon JM, Cushman JC, Cramer GR., J Agric Food Chem 59(1), 2011
PMID: 21128664
Perspectives for production and application of resveratrol.
Kiselev KV., Appl Microbiol Biotechnol 90(2), 2011
PMID: 21360145
Occurrence and estimation of trans-resveratrol in one-year-old canes from seven major Chinese grape producing regions.
Zhang A, Fang Y, Li X, Meng J, Wang H, Li H, Zhang Z, Guo Z., Molecules 16(4), 2011
PMID: 21455097
Cre-mediated seed-specific transgene excision in tobacco.
Kopertekh L, Schulze K, Frolov A, Strack D, Broer I, Schiemann J., Plant Mol Biol 72(6), 2010
PMID: 20076992
Molecular engineering of resveratrol in plants.
Delaunois B, Cordelier S, Conreux A, Clément C, Jeandet P., Plant Biotechnol J 7(1), 2009
PMID: 19021877
Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis.
Kiselev KV, Dubrovina AS, Bulgakov VP., Appl Microbiol Biotechnol 82(4), 2009
PMID: 19043702
Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria x ananassa).
Hanhineva K, Kokko H, Siljanen H, Rogachev I, Aharoni A, Kärenlampi SO., J Exp Bot 60(7), 2009
PMID: 19443619
Potential for metabolic engineering of resveratrol biosynthesis.
Halls C, Yu O., Trends Biotechnol 26(2), 2008
PMID: 18191264
EST analysis of hop glandular trichomes identifies an O-methyltransferase that catalyzes the biosynthesis of xanthohumol.
Nagel J, Culley LK, Lu Y, Liu E, Matthews PD, Stevens JF, Page JE., Plant Cell 20(1), 2008
PMID: 18223037
Efficient and stable transformation of hop (Humulus lupulus L.) var. Eroica by particle bombardment.
Batista D, Fonseca S, Serrazina S, Figueiredo A, Pais MS., Plant Cell Rep 27(7), 2008
PMID: 18369628

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