Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities

Schwekendiek A, Spring O, Heyerick A, Pickel B, Pitsch NT, Peschke F, de Keukeleire D, Weber G (2007)
Journal of agricultural and food chemistry 55(17): 7002-7009.

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Resveratrol, a well-known phytoalexin and antioxidant, is produced by the action of stilbene synthase (STS) in some plant species. Hop (Humulus lupulus L.) plants of the Tettnang variety were transformed with a gene encoding for STS from grapevine. Under the control of the constitutive 35S cauliflower mosaic virus promoter, expression of the transgene resulted in accumulation of resveratrol and high levels of its glycosylated derivatives in leaves and inflorescences. Piceid, the predominant derivative, reached a concentration of up to 560 microg/g of fresh weight (f.w.) in hop cones, whereas no stilbenes were detected in nontransformed controls (wild-type). In transgenic plants the amounts of alpha- and beta-acids, naringenin chalcone, and prenylated flavonoids did not change significantly when compared with nontransformed plants. Transgenic plants showed normal morphology and flower development as did the nontransformed controls. The results clearly show that in hop constitutive expression of sts interferes neither with plant development nor with the biosynthesis of secondary metabolites relevant for the brewing industry. Since resveratrol is a well-known phytoalexin and antioxidant, sts transgenic hop plants could display enhanced pathogen resistance against microbial pathogens, exhibit new beneficial properties for health, and open new venues for metabolic engineering.
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Schwekendiek A, Spring O, Heyerick A, et al. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 2007;55(17):7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., et al. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), 7002-7009. doi:10.1021/jf070509e
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., and Weber, G. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry 55, 7002-7009.
Schwekendiek, A., et al., 2007. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), p 7002-7009.
A. Schwekendiek, et al., “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”, Journal of agricultural and food chemistry, vol. 55, 2007, pp. 7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N.T., Peschke, F., de Keukeleire, D., Weber, G.: Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 55, 7002-7009 (2007).
Schwekendiek, Axel, Spring, Otmar, Heyerick, Arne, Pickel, Benjamin, Pitsch, Nicola T, Peschke, Florian, de Keukeleire, Denis, and Weber, Gerd. “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”. Journal of agricultural and food chemistry 55.17 (2007): 7002-7009.
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PMID: 18369628

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