Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities

Schwekendiek A, Spring O, Heyerick A, Pickel B, Pitsch NT, Peschke F, de Keukeleire D, Weber G (2007)
Journal of agricultural and food chemistry 55(17): 7002-7009.

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Resveratrol, a well-known phytoalexin and antioxidant, is produced by the action of stilbene synthase (STS) in some plant species. Hop (Humulus lupulus L.) plants of the Tettnang variety were transformed with a gene encoding for STS from grapevine. Under the control of the constitutive 35S cauliflower mosaic virus promoter, expression of the transgene resulted in accumulation of resveratrol and high levels of its glycosylated derivatives in leaves and inflorescences. Piceid, the predominant derivative, reached a concentration of up to 560 microg/g of fresh weight (f.w.) in hop cones, whereas no stilbenes were detected in nontransformed controls (wild-type). In transgenic plants the amounts of alpha- and beta-acids, naringenin chalcone, and prenylated flavonoids did not change significantly when compared with nontransformed plants. Transgenic plants showed normal morphology and flower development as did the nontransformed controls. The results clearly show that in hop constitutive expression of sts interferes neither with plant development nor with the biosynthesis of secondary metabolites relevant for the brewing industry. Since resveratrol is a well-known phytoalexin and antioxidant, sts transgenic hop plants could display enhanced pathogen resistance against microbial pathogens, exhibit new beneficial properties for health, and open new venues for metabolic engineering.
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Schwekendiek A, Spring O, Heyerick A, et al. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 2007;55(17):7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., et al. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), 7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N. T., Peschke, F., de Keukeleire, D., and Weber, G. (2007). Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry 55, 7002-7009.
Schwekendiek, A., et al., 2007. Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry, 55(17), p 7002-7009.
A. Schwekendiek, et al., “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”, Journal of agricultural and food chemistry, vol. 55, 2007, pp. 7002-7009.
Schwekendiek, A., Spring, O., Heyerick, A., Pickel, B., Pitsch, N.T., Peschke, F., de Keukeleire, D., Weber, G.: Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities. Journal of agricultural and food chemistry. 55, 7002-7009 (2007).
Schwekendiek, Axel, Spring, Otmar, Heyerick, Arne, Pickel, Benjamin, Pitsch, Nicola T, Peschke, Florian, de Keukeleire, Denis, and Weber, Gerd. “Constitutive expression of a grapevine stilbene synthase gene in transgenic hop (Humulus lupulus L.) yields resveratrol and its derivatives in substantial quantities”. Journal of agricultural and food chemistry 55.17 (2007): 7002-7009.
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PMID: 26302213
Resveratrol biosynthesis: plant metabolic engineering for nutritional improvement of food.
Giovinazzo G, Ingrosso I, Paradiso A, De Gara L, Santino A., Plant Foods Hum Nutr 67(3), 2012
PMID: 22777386
Metabolic engineering of yeast and plants for the production of the biologically active hydroxystilbene, resveratrol.
Jeandet P, Delaunois B, Aziz A, Donnez D, Vasserot Y, Cordelier S, Courot E., J. Biomed. Biotechnol. 2012(), 2012
PMID: 22654481
Phytoalexins in defense against pathogens.
Ahuja I, Kissen R, Bones AM., Trends Plant Sci. 17(2), 2012
PMID: 22209038
Cloning a peanut resveratrol synthase gene and its expression in purple sweet potato.
Pan LP, Yu SL, Chen CJ, Li H, Wu YL, Li HH., Plant Cell Rep. 31(1), 2012
PMID: 21932029
Expression pattern, genomic structure, and promoter analysis of the gene encoding stilbene synthase from Chinese wild Vitis pseudoreticulata.
Xu W, Yu Y, Zhou Q, Ding J, Dai L, Xie X, Xu Y, Zhang C, Wang Y., J. Exp. Bot. 62(8), 2011
PMID: 21504880
Perspectives for production and application of resveratrol.
Kiselev KV., Appl. Microbiol. Biotechnol. 90(2), 2011
PMID: 21360145
Water deficit increases stilbene metabolism in Cabernet Sauvignon berries.
Deluc LG, Decendit A, Papastamoulis Y, Merillon JM, Cushman JC, Cramer GR., J. Agric. Food Chem. 59(1), 2011
PMID: 21128664
Cre-mediated seed-specific transgene excision in tobacco.
Kopertekh L, Schulze K, Frolov A, Strack D, Broer I, Schiemann J., Plant Mol. Biol. 72(6), 2010
PMID: 20076992
Stilbene synthase gene transfer caused alterations in the phenylpropanoid metabolism of transgenic strawberry (Fragaria x ananassa).
Hanhineva K, Kokko H, Siljanen H, Rogachev I, Aharoni A, Karenlampi SO., J. Exp. Bot. 60(7), 2009
PMID: 19443619
Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis.
Kiselev KV, Dubrovina AS, Bulgakov VP., Appl. Microbiol. Biotechnol. 82(4), 2009
PMID: 19043702
Molecular engineering of resveratrol in plants.
Delaunois B, Cordelier S, Conreux A, Clement C, Jeandet P., Plant Biotechnol. J. 7(1), 2009
PMID: 19021877
Efficient and stable transformation of hop (Humulus lupulus L.) var. Eroica by particle bombardment.
Batista D, Fonseca S, Serrazina S, Figueiredo A, Pais MS., Plant Cell Rep. 27(7), 2008
PMID: 18369628
Potential for metabolic engineering of resveratrol biosynthesis.
Halls C, Yu O., Trends Biotechnol. 26(2), 2008
PMID: 18191264

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