The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin

Jankowitsch F, Schwarz J, Rückert C, Gust B, Szczepanowski R, Blom J, Pelzer S, Kalinowski J, Mack M (2012)
Journal of bacteriology 194(24): 6818-6827.

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Abstract
Streptoymces davawensis JCM 4913 synthesizes the antibiotic roseoflavin, a structural riboflavin (vitamin B(2)) analog. Here we report the 9,466,619 base pair linear chromosome of S. davawensis JCM 4913 and a 89,331 base pair linear plasmid. The sequence has an average G + C content of 70.58% and contains six rRNA operons (16S-23S-5S) and 69 tRNA genes. The 8,616 predicted protein-coding sequences include 32 clusters coding for secondary metabolites several of which are unique to S. davawensis. The chromosome contains long terminal inverted repeats of 33,255 bp each and atypical telomeres. Sequence analysis with regard to riboflavin biosynthesis revealed three different patterns of gene organization in Streptomyces species. Heterologous expression of a set of genes present on a subgenomic fragment of S. davawensis resulted in the production of roseoflavin by the host Streptomyces coelicolor M1152. Phylogenetic analysis revealed that S. davawensis is a close relative to Streptomyces cinnabarinus and, much to our surprise, we found that the latter bacterium is a roseoflavin producer as well.
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Jankowitsch F, Schwarz J, Rückert C, et al. The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. Journal of bacteriology. 2012;194(24):6818-6827.
Jankowitsch, F., Schwarz, J., Rückert, C., Gust, B., Szczepanowski, R., Blom, J., Pelzer, S., et al. (2012). The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. Journal of bacteriology, 194(24), 6818-6827.
Jankowitsch, F., Schwarz, J., Rückert, C., Gust, B., Szczepanowski, R., Blom, J., Pelzer, S., Kalinowski, J., and Mack, M. (2012). The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. Journal of bacteriology 194, 6818-6827.
Jankowitsch, F., et al., 2012. The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. Journal of bacteriology, 194(24), p 6818-6827.
F. Jankowitsch, et al., “The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin”, Journal of bacteriology, vol. 194, 2012, pp. 6818-6827.
Jankowitsch, F., Schwarz, J., Rückert, C., Gust, B., Szczepanowski, R., Blom, J., Pelzer, S., Kalinowski, J., Mack, M.: The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin. Journal of bacteriology. 194, 6818-6827 (2012).
Jankowitsch, Frank, Schwarz, Julia, Rückert, Christian, Gust, Bertolt, Szczepanowski, Rafael, Blom, Jochen, Pelzer, Stefan, Kalinowski, Jörn, and Mack, Matthias. “The genome sequence of the bacterium Streptomyces davawensis JCM 4913 and heterologous production of the unique antibiotic roseoflavin”. Journal of bacteriology 194.24 (2012): 6818-6827.
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