Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae

Strobel T, Al-Dilaimi A, Blom J, Gessner A, Kalinowski J, Luzhetska M, Pühler A, Szczepanowski R, Bechthold A, Rückert C (2012)
BMC Genomics 13(1).

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ABSTRACT: BACKGROUND: The genus Saccharothrix is a representative of the family Pseudonocardiaceae, known to include producer strains of a wide variety of potent antibiotics. Saccharothrix espanaensis produces both saccharomicins A and B of the promising new class of heptadecaglycoside antibiotics, active against both bacteria and yeast. RESULTS: To better assess its capabilities, the complete genome sequence of S. espanaensis was established. With a size of 9,360,653 bp, coding for 8,501 genes, it stands alongside other Pseudonocardiaceae with large genomes. Besides a predicted core genome of 810 genes shared in the family, S. espanaensis has a large number of accessory genes: 2,967 singletons when compared to the family, of which 1,292 have no clear orthologs in the RefSeq database. The genome analysis revealed the presence of 26 biosynthetic gene clusters potentially encoding secondary metabolites. Among them, the cluster coding for the saccharomicins could be identified. CONCLUSION: S. espanaensis is the first completely sequenced species of the genus Saccharothrix. The genome discloses the cluster responsible for the biosynthesis of the saccharomicins, the largest oligosaccharide antibiotic currently identified. Moreover, the genome revealed 25 additional putative secondary metabolite gene clusters further suggesting the strain's potential for natural product synthesis.
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Strobel T, Al-Dilaimi A, Blom J, et al. Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae. BMC Genomics. 2012;13(1).
Strobel, T., Al-Dilaimi, A., Blom, J., Gessner, A., Kalinowski, J., Luzhetska, M., Pühler, A., et al. (2012). Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae. BMC Genomics, 13(1).
Strobel, T., Al-Dilaimi, A., Blom, J., Gessner, A., Kalinowski, J., Luzhetska, M., Pühler, A., Szczepanowski, R., Bechthold, A., and Rückert, C. (2012). Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae. BMC Genomics 13.
Strobel, T., et al., 2012. Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae. BMC Genomics, 13(1).
T. Strobel, et al., “Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae”, BMC Genomics, vol. 13, 2012.
Strobel, T., Al-Dilaimi, A., Blom, J., Gessner, A., Kalinowski, J., Luzhetska, M., Pühler, A., Szczepanowski, R., Bechthold, A., Rückert, C.: Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae. BMC Genomics. 13, (2012).
Strobel, Tina, Al-Dilaimi, Arwa, Blom, Jochen, Gessner, Arne, Kalinowski, Jörn, Luzhetska, Marta, Pühler, Alfred, Szczepanowski, Rafael, Bechthold, Andreas, and Rückert, Christian. “Complete genome sequence of Saccharothrix espanaensis DSM 44229T and comparison to the other completely sequenced Pseudonocardiaceae”. BMC Genomics 13.1 (2012).
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PMID: 25301375
Complete genome sequence of the actinobacterium Amycolatopsis japonica MG417-CF17(T) (=DSM 44213T) producing (S,S)-N,N'-ethylenediaminedisuccinic acid.
Stegmann E, Albersmeier A, Spohn M, Gert H, Weber T, Wohlleben W, Kalinowski J, Ruckert C., J. Biotechnol. 189(), 2014
PMID: 25193710
Bioactive oligosaccharide natural products.
McCranie EK, Bachmann BO., Nat Prod Rep 31(8), 2014
PMID: 24883430
Genomic characteristics and comparative genomics analysis of Penicillium chrysogenum KF-25.
Peng Q, Yuan Y, Gao M, Chen X, Liu B, Liu P, Wu Y, Wu D., BMC Genomics 15(), 2014
PMID: 24555742
MbtH homology codes to identify gifted microbes for genome mining.
Baltz RH., J. Ind. Microbiol. Biotechnol. 41(2), 2014
PMID: 24197465
Tracking down biotransformation to the genetic level: identification of a highly flexible glycosyltransferase from Saccharothrix espanaensis.
Strobel T, Schmidt Y, Linnenbrink A, Luzhetskyy A, Luzhetska M, Taguchi T, Brotz E, Paululat T, Stasevych M, Stanko O, Novikov V, Bechthold A., Appl. Environ. Microbiol. 79(17), 2013
PMID: 23793643

53 References

Data provided by Europe PubMed Central.

The Ribosomal Database Project: improved alignments and new tools for rRNA analysis.
Cole JR, Wang Q, Cardenas E, Fish J, Chai B, Farris RJ, Kulam-Syed-Mohideen AS, McGarrell DM, Marsh T, Garrity GM, Tiedje JM., Nucleic Acids Res. 37(Database issue), 2009
PMID: 19004872
The specificity-conferring code of adenylation domains in nonribosomal peptide synthetases.
Stachelhaus T, Mootz HD, Marahiel MA., Chem. Biol. 6(8), 1999
PMID: 10421756

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