Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*

Heider S, Peters-Wendisch P, Wendisch VF (2012)
BMC Microbiology 12(198).

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Abstract / Bemerkung
Background Corynebacterium glutamicum contains the glycosylated C50 carotenoid decaprenoxanthin as yellow pigment. Starting from isopentenyl pyrophosphate, which is generated in the non-mevalonate pathway, decaprenoxanthin is synthesized via the intermediates farnesyl pyrophosphate, geranylgeranyl pyrophosphate, lycopene and flavuxanthin. Results Here, we showed that the genes of the carotenoid gene cluster crtE-cg0722-crtBIYeYfEb are co-transcribed and characterized defined gene deletion mutants. Gene deletion analysis revealed that crtI, crtEb, and crtYeYf, respectively, code for the only phytoene desaturase, lycopene elongase, and carotenoid C45/C50 epsilon-cyclase, respectively. However, the genome of C. glutamicum also encodes a second carotenoid gene cluster comprising crtB2I2-1/2 shown to be co-transcribed, as well. Ectopic expression of crtB2 could compensate for the lack of phytoene synthase CrtB in C. glutamicum DeltacrtB, thus, C. glutamicum possesses two functional phytoene synthases, namely CrtB and CrtB2. Genetic evidence for a crtI2-1/2 encoded phytoene desaturase could not be obtained since plasmid-borne expression of crtI2-1/2 did not compensate for the lack of phytoene desaturase CrtI in C. glutamicum DeltacrtI. The potential of C. glutamicum to overproduce carotenoids was estimated with lycopene as example. Deletion of the gene crtEb prevented conversion of lycopene to decaprenoxanthin and entailed accumulation of lycopene to 0.03 +/- 0.01 mg/g cell dry weight (CDW). When the genes crtE, crtB and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were overexpressed in C. glutamicum DeltacrtEb intensely red-pigmented cells and an 80 fold increased lycopene content of 2.4 +/- 0.3 mg/g CDW were obtained. Conclusion C. glutamicum possesses a certain degree of redundancy in the biosynthesis of the C50 carotenoid decaprenoxanthin as it possesses two functional phytoene synthase genes. Already metabolic engineering of only the terminal reactions leading to lycopene resulted in considerable lycopene production indicating that C. glutamicum may serve as a potential host for carotenoid production.
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Zeitschriftentitel
BMC Microbiology
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12
Zeitschriftennummer
198
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Heider S, Peters-Wendisch P, Wendisch VF. Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology. 2012;12(198).
Heider, S., Peters-Wendisch, P., & Wendisch, V. F. (2012). Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology, 12(198). doi:10.1186/1471-2180-12-198
Heider, S., Peters-Wendisch, P., and Wendisch, V. F. (2012). Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology 12.
Heider, S., Peters-Wendisch, P., & Wendisch, V.F., 2012. Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology, 12(198).
S. Heider, P. Peters-Wendisch, and V.F. Wendisch, “Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*”, BMC Microbiology, vol. 12, 2012.
Heider, S., Peters-Wendisch, P., Wendisch, V.F.: Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology. 12, (2012).
Heider, Sabine, Peters-Wendisch, Petra, and Wendisch, Volker F. “Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*”. BMC Microbiology 12.198 (2012).
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29 Zitationen in Europe PMC

Daten bereitgestellt von Europe PubMed Central.

Production and glucosylation of C50 and C 40 carotenoids by metabolically engineered Corynebacterium glutamicum.
Heider SA, Peters-Wendisch P, Netzer R, Stafnes M, Brautaset T, Wendisch VF., Appl Microbiol Biotechnol 98(3), 2014
PMID: 24270893
Development of fatty acid-producing Corynebacterium glutamicum strains.
Takeno S, Takasaki M, Urabayashi A, Mimura A, Muramatsu T, Mitsuhashi S, Ikeda M., Appl Environ Microbiol 79(21), 2013
PMID: 23995924
Genomes of "Spiribacter", a streamlined, successful halophilic bacterium.
López-Pérez M, Ghai R, Leon MJ, Rodríguez-Olmos Á, Copa-Patiño JL, Soliveri J, Sanchez-Porro C, Ventosa A, Rodriguez-Valera F., BMC Genomics 14(), 2013
PMID: 24225341

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