Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*

Heider S, Peters-Wendisch P, Wendisch VF (2012)
BMC Microbiology 12(198).

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Abstract
Background Corynebacterium glutamicum contains the glycosylated C50 carotenoid decaprenoxanthin as yellow pigment. Starting from isopentenyl pyrophosphate, which is generated in the non-mevalonate pathway, decaprenoxanthin is synthesized via the intermediates farnesyl pyrophosphate, geranylgeranyl pyrophosphate, lycopene and flavuxanthin. Results Here, we showed that the genes of the carotenoid gene cluster crtE-cg0722-crtBIYeYfEb are co-transcribed and characterized defined gene deletion mutants. Gene deletion analysis revealed that crtI, crtEb, and crtYeYf, respectively, code for the only phytoene desaturase, lycopene elongase, and carotenoid C45/C50 epsilon-cyclase, respectively. However, the genome of C. glutamicum also encodes a second carotenoid gene cluster comprising crtB2I2-1/2 shown to be co-transcribed, as well. Ectopic expression of crtB2 could compensate for the lack of phytoene synthase CrtB in C. glutamicum DeltacrtB, thus, C. glutamicum possesses two functional phytoene synthases, namely CrtB and CrtB2. Genetic evidence for a crtI2-1/2 encoded phytoene desaturase could not be obtained since plasmid-borne expression of crtI2-1/2 did not compensate for the lack of phytoene desaturase CrtI in C. glutamicum DeltacrtI. The potential of C. glutamicum to overproduce carotenoids was estimated with lycopene as example. Deletion of the gene crtEb prevented conversion of lycopene to decaprenoxanthin and entailed accumulation of lycopene to 0.03 +/- 0.01 mg/g cell dry weight (CDW). When the genes crtE, crtB and crtI for conversion of geranylgeranyl pyrophosphate to lycopene were overexpressed in C. glutamicum DeltacrtEb intensely red-pigmented cells and an 80 fold increased lycopene content of 2.4 +/- 0.3 mg/g CDW were obtained. Conclusion C. glutamicum possesses a certain degree of redundancy in the biosynthesis of the C50 carotenoid decaprenoxanthin as it possesses two functional phytoene synthase genes. Already metabolic engineering of only the terminal reactions leading to lycopene resulted in considerable lycopene production indicating that C. glutamicum may serve as a potential host for carotenoid production.
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Heider S, Peters-Wendisch P, Wendisch VF. Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology. 2012;12(198).
Heider, S., Peters-Wendisch, P., & Wendisch, V. F. (2012). Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology, 12(198).
Heider, S., Peters-Wendisch, P., and Wendisch, V. F. (2012). Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology 12.
Heider, S., Peters-Wendisch, P., & Wendisch, V.F., 2012. Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology, 12(198).
S. Heider, P. Peters-Wendisch, and V.F. Wendisch, “Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*”, BMC Microbiology, vol. 12, 2012.
Heider, S., Peters-Wendisch, P., Wendisch, V.F.: Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*. BMC Microbiology. 12, (2012).
Heider, Sabine, Peters-Wendisch, Petra, and Wendisch, Volker F. “Carotenoid biosynthesis and overproduction in *Corynebacterium glutamicum*”. BMC Microbiology 12.198 (2012).
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21 Citations in Europe PMC

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PMID: 27376307
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de Oliveira JD, Carvalho LS, Gomes AM, Queiroz LR, Magalhaes BS, Parachin NS., Microb. Cell Fact. 15(1), 2016
PMID: 27370777
Complete biosynthetic pathway of the C50 carotenoid bacterioruberin from lycopene in the extremely halophilic archaeon Haloarcula japonica.
Yang Y, Yatsunami R, Ando A, Miyoko N, Fukui T, Takaichi S, Nakamura S., J. Bacteriol. 197(9), 2015
PMID: 25712483
Genomic and phenotypic insights into the ecology of Arthrobacter from Antarctic soils.
Dsouza M, Taylor MW, Turner SJ, Aislabie J., BMC Genomics 16(), 2015
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PMID: 25191655
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Heider SA, Peters-Wendisch P, Beekwilder J, Wendisch VF., FEBS J. 281(21), 2014
PMID: 25181035
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PMID: 24930112
Production of the sesquiterpene (+)-valencene by metabolically engineered Corynebacterium glutamicum.
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PMID: 24910970
Metabolic engineering for the microbial production of carotenoids and related products with a focus on the rare C50 carotenoids.
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PMID: 24687754
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PMID: 23995924

50 References

Data provided by Europe PubMed Central.


AUTHOR UNKNOWN, 2005
Sigma factors and promoters in Corynebacterium glutamicum.
Patek M, Nesvera J., J. Biotechnol. 154(2-3), 2011
PMID: 21277915
Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli.
Alper H, Jin YS, Moxley JF, Stephanopoulos G., Metab. Eng. 7(3), 2005
PMID: 15885614
Increase of lycopene production by supplementing auxiliary carbon sources in metabolically engineered Escherichia coli.
Kim YS, Lee JH, Kim NH, Yeom SJ, Kim SW, Oh DK., Appl. Microbiol. Biotechnol. 90(2), 2011
PMID: 21246354
The chemistry of novel xanthophyll carotenoids.
Jackson H, Braun CL, Ernst H., Am. J. Cardiol. 101(10A), 2008
PMID: 18474275
Antioxidant activities of astaxanthin and related carotenoids.
Naguib YM., J. Agric. Food Chem. 48(4), 2000
PMID: 10775364
Antioxidant activities of carotenes and xanthophylls.
Miller NJ, Sampson J, Candeias LP, Bramley PM, Rice-Evans CA., FEBS Lett. 384(3), 1996
PMID: 8617362
Experiments
AUTHOR UNKNOWN, 2005

AUTHOR UNKNOWN, 2001
Studies on transformation of Escherichia coli with plasmids.
Hanahan D., J. Mol. Biol. 166(4), 1983
PMID: 6345791
Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis.
Netzer R, Krause M, Rittmann D, Peters-Wendisch PG, Eggeling L, Wendisch VF, Sahm H., Arch. Microbiol. 182(5), 2004
PMID: 15375646
Identification and characterization of the dicarboxylate uptake system DccT in Corynebacterium glutamicum.
Youn JW, Jolkver E, Kramer R, Marin K, Wendisch VF., J. Bacteriol. 190(19), 2008
PMID: 18658264
Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production.
Stansen C, Uy D, Delaunay S, Eggeling L, Goergen JL, Wendisch VF., Appl. Environ. Microbiol. 71(10), 2005
PMID: 16204505
Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum.
Peters-Wendisch PG, Schiel B, Wendisch VF, Katsoulidis E, Mockel B, Sahm H, Eikmanns BJ., J. Mol. Microbiol. Biotechnol. 3(2), 2001
PMID: 11321586
Basic local alignment search tool.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ., J. Mol. Biol. 215(3), 1990
PMID: 2231712
Taxonomical studies on glutamic acid producing bacteria
AUTHOR UNKNOWN, 1967

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