Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling

Flügel M, Becker A, Gartemann K-H, Eichenlaub R (2012)
Journal of Biotechnology 160(1-2): 42-54.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Genome-wide expression profiles of the phytopathogenic actinomycete Clavibacter michiganensis subsp. michiganensis (Cmm) strain NCPPB382 were analyzed using a 70mer oligonucleotide microarray. Cmm causes bacterial wilt and canker of tomato, a systemic disease leading to substantial economic losses worldwide. Global gene expression was monitored in vitro after long-and short-term incubation with tomato homogenate to simulate conditions in planta and in vivo ten days after inoculation of tomatoes. Surprisingly, both in the presence of tomato homogenate and in planta known virulence genes (celA, chpC, ppaA/C) were down-regulated indicating that the encoded extracellular enzymes are dispensable in late infection stages where plant tissue has already been heavily destroyed. In contrast, some genes of the tomA-region which are involved in sugar metabolism showed an enhanced RNA-level after permanent growth in supplemented medium. Therefore, these genes may be important for utilization of plant derived nutrients. In the plant Cmm exhibited an expression profile completely different from that in vitro. Especially, the strong expression of genes of the wco-cluster (extracellular polysaccharide II), 10 genes encoding surface or pilus assembly proteins, and CMM_2382, coding for a putative perforin suggest a possible role of these genes in the plant-pathogenic interaction. (C) 2012 Elsevier B.V. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
Journal of Biotechnology
Band
160
Zeitschriftennummer
1-2
Seite
42-54
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Flügel M, Becker A, Gartemann K-H, Eichenlaub R. Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling. Journal of Biotechnology. 2012;160(1-2):42-54.
Flügel, M., Becker, A., Gartemann, K. - H., & Eichenlaub, R. (2012). Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling. Journal of Biotechnology, 160(1-2), 42-54. doi:10.1016/j.jbiotec.2012.01.023
Flügel, M., Becker, A., Gartemann, K. - H., and Eichenlaub, R. (2012). Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling. Journal of Biotechnology 160, 42-54.
Flügel, M., et al., 2012. Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling. Journal of Biotechnology, 160(1-2), p 42-54.
M. Flügel, et al., “Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling”, Journal of Biotechnology, vol. 160, 2012, pp. 42-54.
Flügel, M., Becker, A., Gartemann, K.-H., Eichenlaub, R.: Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling. Journal of Biotechnology. 160, 42-54 (2012).
Flügel, Monika, Becker, Anke, Gartemann, Karl-Heinz, and Eichenlaub, Rudolf. “Analysis of the interaction of Clavibacter michiganensis subsp michiganensis with its host plant tomato by genome-wide expression profiling”. Journal of Biotechnology 160.1-2 (2012): 42-54.

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A Proteomic Study of Clavibacter Michiganensis Subsp. Michiganensis Culture Supernatants.
Hiery E, Poetsch A, Moosbauer T, Amin B, Hofmann J, Burkovski A., Proteomes 3(4), 2015
PMID: 28248277
Evaluation of a SUMO E2 Conjugating Enzyme Involved in Resistance to Clavibacter michiganensis Subsp. michiganensis in Solanum peruvianum, Through a Tomato Mottle Virus VIGS Assay.
Esparza-Araiza MJ, Bañuelos-Hernández B, Argüello-Astorga GR, Lara-Ávila JP, Goodwin PH, Isordia-Jasso MI, Castillo-Collazo R, Rougon-Cardoso A, Alpuche-Solís ÁG., Front Plant Sci 6(), 2015
PMID: 26734014
The leafy gall syndrome induced by Rhodococcus fascians.
Stes E, Francis I, Pertry I, Dolzblasz A, Depuydt S, Vereecke D., FEMS Microbiol Lett 342(2), 2013
PMID: 23480693
Genome-wide transcriptome analysis of Clavibacter michiganensis subsp. michiganensis grown in xylem mimicking medium.
Hiery E, Adam S, Reid S, Hofmann J, Sonnewald S, Burkovski A., J Biotechnol 168(4), 2013
PMID: 24060828

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