Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora

Rezzonico F, Braun-Kiewnick A, Mann RA, Rodoni B, Goesmann A, Duffy B, Smits THM (2012)
Molecular plant pathology 13(8): 975-984.

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Abstract
Comparative genomic analysis revealed differences in the lipopolysaccharide (LPS) biosynthesis gene cluster between the Rubus-infecting strain ATCC BAA-2158 and the Spiraeoideae-infecting strain CFBP 1430 of Erwinia amylovora. These differences corroborate rpoB-based phylogenetic clustering of E. amylovora into four different groups and enable the discrimination of Spiraeoideae- and Rubus-infecting strains. The structure of the differences between the two groups supports the hypothesis that adaptation to Rubus spp. took place after species separation of E. amylovora and E. pyrifoliae that contrasts with a recently proposed scenario, based on CRISPR data, in which the shift to domesticated apple would have caused an evolutionary bottleneck in the Spiraeoideae-infecting strains of E. amylovora which would be a much earlier event. In the core region of the LPS biosynthetic gene cluster, Spiraeoideae-infecting strains encode three glycosyltransferases and an LPS ligase (Spiraeoideae-type waaL), whereas Rubus-infecting strains encode two glycosyltransferases and a different LPS ligase (Rubus-type waaL). These coding domains share little to no homology at the amino acid level between Rubus- and Spiraeoideae-infecting strains, and this genotypic difference was confirmed by polymerase chain reaction analysis of the associated DNA region in 31 Rubus- and Spiraeoideae-infecting strains. The LPS biosynthesis gene cluster may thus be used as a molecular marker to distinguish between Rubus- and Spiraeoideae-infecting strains of E. amylovora using primers designed in this study.
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Rezzonico F, Braun-Kiewnick A, Mann RA, et al. Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora. Molecular plant pathology. 2012;13(8):975-984.
Rezzonico, F., Braun-Kiewnick, A., Mann, R. A., Rodoni, B., Goesmann, A., Duffy, B., & Smits, T. H. M. (2012). Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora. Molecular plant pathology, 13(8), 975-984.
Rezzonico, F., Braun-Kiewnick, A., Mann, R. A., Rodoni, B., Goesmann, A., Duffy, B., and Smits, T. H. M. (2012). Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora. Molecular plant pathology 13, 975-984.
Rezzonico, F., et al., 2012. Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora. Molecular plant pathology, 13(8), p 975-984.
F. Rezzonico, et al., “Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora”, Molecular plant pathology, vol. 13, 2012, pp. 975-984.
Rezzonico, F., Braun-Kiewnick, A., Mann, R.A., Rodoni, B., Goesmann, A., Duffy, B., Smits, T.H.M.: Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora. Molecular plant pathology. 13, 975-984 (2012).
Rezzonico, Fabio, Braun-Kiewnick, Andrea, Mann, Rachel A, Rodoni, Brendan, Goesmann, Alexander, Duffy, Brion, and Smits, Theo H M. “Lipopolysaccharide biosynthesis genes discriminate between Rubus- and Spiraeoideae-infective genotypes of Erwinia amylovora”. Molecular plant pathology 13.8 (2012): 975-984.
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Virulence Factors of Erwinia amylovora: A Review.
Pique N, Minana-Galbis D, Merino S, Tomas JM., Int J Mol Sci 16(6), 2015
PMID: 26057748
Phylogeography and population structure of the biologically invasive phytopathogen Erwinia amylovora inferred using minisatellites.
Buhlmann A, Dreo T, Rezzonico F, Pothier JF, Smits TH, Ravnikar M, Frey JE, Duffy B., Environ. Microbiol. 16(7), 2014
PMID: 24112873

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