Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance

Ritzefeld M, Sewald N (2012)
Journal of amino acids 2012: 1-19.

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Several proteins, like transcription factors, bind to certain DNA sequences, thereby regulating biochemical pathways that determine the fate of the corresponding cell. Due to these key positions, it is indispensable to analyze protein-DNA interactions and to identify their mode of action. Surface plasmon resonance is a label-free method that facilitates the elucidation of real-time kinetics of biomolecular interactions. In this article, we focus on this biosensor-based method and provide a detailed guide how SPR can be utilized to study binding of proteins to oligonucleotides. After a description of the physical phenomenon and the instrumental realization including fiber-optic-based SPR and SPR imaging, we will continue with a survey of immobilization methods. Subsequently, we will focus on the optimization of the experiment, expose pitfalls, and introduce how data should be analyzed and published. Finally, we summarize several interesting publications of the last decades dealing with protein-DNA and RNA interaction analysis by SPR.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Ritzefeld M, Sewald N. Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of amino acids. 2012;2012:1-19.
Ritzefeld, M., & Sewald, N. (2012). Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of amino acids, 2012, 1-19.
Ritzefeld, M., and Sewald, N. (2012). Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of amino acids 2012, 1-19.
Ritzefeld, M., & Sewald, N., 2012. Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of amino acids, 2012, p 1-19.
M. Ritzefeld and N. Sewald, “Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance”, Journal of amino acids, vol. 2012, 2012, pp. 1-19.
Ritzefeld, M., Sewald, N.: Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance. Journal of amino acids. 2012, 1-19 (2012).
Ritzefeld, Markus, and Sewald, Norbert. “Real-Time Analysis of Specific Protein-DNA Interactions with Surface Plasmon Resonance”. Journal of amino acids 2012 (2012): 1-19.
This data publication is cited in the following publications:
This publication cites the following data publications:

5 Citations in Europe PMC

Data provided by Europe PubMed Central.

Surface plasmon resonance: a label-free tool for cellular analysis.
Zeidan E, Kepley CL, Sayes C, Sandros MG., Nanomedicine (Lond) 10(11), 2015
PMID: 26080702
Surface plasmon resonance for cell-based clinical diagnosis.
Yanase Y, Hiragun T, Ishii K, Kawaguchi T, Yanase T, Kawai M, Sakamoto K, Hide M., Sensors (Basel) 14(3), 2014
PMID: 24618778
Production of pure and functional RNA for in vitro reconstitution experiments.
Edelmann FT, Niedner A, Niessing D., Methods 65(3), 2014
PMID: 24021718

89 References

Data provided by Europe PubMed Central.

Lactose repressor-operator DNA interactions: kinetic analysis by a surface plasmon resonance biosensor.
Bondeson K, Frostell-Karlsson A, Fagerstam L, Magnusson G., Anal. Biochem. 214(1), 1993
PMID: 8250230
Analysis of the DNA-binding domain of Escherichia coli DnaA protein.
Blaesing F, Weigel C, Welzeck M, Messer W., Mol. Microbiol. 36(3), 2000
PMID: 10844646
Fiber optic SPR biosensing of DNA hybridization and DNA-protein interactions.
Pollet J, Delport F, Janssen KP, Jans K, Maes G, Pfeiffer H, Wevers M, Lammertyn J., Biosens Bioelectron 25(4), 2009
PMID: 19775884
Double recognition of oligonucleotide and protein in the detection of DNA methylation with surface plasmon resonance biosensors.
Pan S, Xu J, Shu Y, Wang F, Xia W, Ding Q, Xu T, Zhao C, Zhang M, Huang P, Lu S., Biosens Bioelectron 26(2), 2010
PMID: 20810273
A dual surface plasmon resonance assay for the determination of ribonuclease H activity.
Sipova H, Vaisocherova H, Stepanek J, Homola J., Biosens Bioelectron 26(4), 2010
PMID: 20829018
Real-time monitoring of solid-phase PCR using fiber-optic SPR.
Pollet J, Janssen KP, Knez K, Lammertyn J., Small 7(8), 2011
PMID: 21394905
Minor groove recognition is important for the transcription factor PhoB: a surface plasmon resonance study.
Ritzefeld M, Wollschlager K, Niemann G, Anselmetti D, Sewald N., Mol Biosyst 7(11), 2011
PMID: 21912786

Export

0 Marked Publications

Open Data PUB

Sources

PMID: 22500214
PubMed | Europe PMC

Search this title in

Google Scholar