Hidden Multiple Bond Effects in Dynamic Force Spectroscopy

Getfert S, Reimann P (2012)
Biophysical Journal 102(5): 1184-1193.

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In dynamic force spectroscopy, a (bio-) molecular complex is subjected to a steadily increasing force until the chemical bond breaks. Repeating the same experiment many times results in a broad distribution of rupture forces, whose quantitative interpretation represents a formidable theoretical challenge. In this study we address the situation that more than a single molecular bond is involved in one experimental run, giving rise to multiple rupture events that are even more difficult to analyze and thus are usually eliminated as far as possible from the further evaluation of the experimental data. We develop and numerically solve a detailed model of a complete dynamic force spectroscopy experiment including a possible clustering of molecules on the substrate surface, the formation of bonds, their dissociation under load, and the postprocessing of the force extension curves. We show that the data, remaining after elimination of obvious multiple rupture events, may still contain a considerable number of hidden multiple bonds, which are experimentally indistinguishable from true single bonds, but which have considerable effects on the resulting rupture force statistics and its consistent theoretical interpretation.
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Getfert S, Reimann P. Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal. 2012;102(5):1184-1193.
Getfert, S., & Reimann, P. (2012). Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal, 102(5), 1184-1193. doi:10.1016/j.bpj.2012.01.037
Getfert, S., and Reimann, P. (2012). Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal 102, 1184-1193.
Getfert, S., & Reimann, P., 2012. Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal, 102(5), p 1184-1193.
S. Getfert and P. Reimann, “Hidden Multiple Bond Effects in Dynamic Force Spectroscopy”, Biophysical Journal, vol. 102, 2012, pp. 1184-1193.
Getfert, S., Reimann, P.: Hidden Multiple Bond Effects in Dynamic Force Spectroscopy. Biophysical Journal. 102, 1184-1193 (2012).
Getfert, Sebastian, and Reimann, Peter. “Hidden Multiple Bond Effects in Dynamic Force Spectroscopy”. Biophysical Journal 102.5 (2012): 1184-1193.
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