Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium

Wu ML, van Teeseling MCF, Willems MJR, van Donselaar EG, Klingl A, Rachel R, Geerts WJC, Jetten MSM, Strous M, van Niftrik L (2012)
Journal of Bacteriology 194(2): 284-291.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ;
Abstract
"Candidatus Methylomirabilis oxyfera" is a newly discovered denitrifying methanotroph that is unrelated to previously known methanotrophs. This bacterium is a member of the NC10 phylum and couples methane oxidation to denitrification through a newly discovered intra-aerobic pathway. In the present study, we report the first ultrastructural study of "Ca. Methylomirabilis oxyfera" using scanning electron microscopy, transmission electron microscopy, and electron tomography in combination with different sample preparation methods. We observed that "Ca. Methylomirabilis oxyfera" cells possess an atypical polygonal shape that is distinct from other bacterial shapes described so far. Also, an additional layer was observed as the outermost sheath, which might represent a (glyco) protein surface layer. Further, intracytoplasmic membranes, which are a common feature among proteobacterial methanotrophs, were never observed under the current growth conditions. Our results indicate that "Ca. Methylomirabilis oxyfera" is ultrastructurally distinct from other bacteria by its atypical cell shape and from the classical proteobacterial methanotrophs by its apparent lack of intracytoplasmic membranes.
Publishing Year
ISSN
PUB-ID

Cite this

Wu ML, van Teeseling MCF, Willems MJR, et al. Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. Journal of Bacteriology. 2012;194(2):284-291.
Wu, M. L., van Teeseling, M. C. F., Willems, M. J. R., van Donselaar, E. G., Klingl, A., Rachel, R., Geerts, W. J. C., et al. (2012). Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. Journal of Bacteriology, 194(2), 284-291.
Wu, M. L., van Teeseling, M. C. F., Willems, M. J. R., van Donselaar, E. G., Klingl, A., Rachel, R., Geerts, W. J. C., Jetten, M. S. M., Strous, M., and van Niftrik, L. (2012). Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. Journal of Bacteriology 194, 284-291.
Wu, M.L., et al., 2012. Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. Journal of Bacteriology, 194(2), p 284-291.
M.L. Wu, et al., “Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium”, Journal of Bacteriology, vol. 194, 2012, pp. 284-291.
Wu, M.L., van Teeseling, M.C.F., Willems, M.J.R., van Donselaar, E.G., Klingl, A., Rachel, R., Geerts, W.J.C., Jetten, M.S.M., Strous, M., van Niftrik, L.: Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium. Journal of Bacteriology. 194, 284-291 (2012).
Wu, Ming L., van Teeseling, Muriel C. F., Willems, Marieke J. R., van Donselaar, Elly G., Klingl, Andreas, Rachel, Reinhard, Geerts, Willie J. C., Jetten, Mike S. M., Strous, Marc, and van Niftrik, Laura. “Ultrastructure of the Denitrifying Methanotroph "Candidatus Methylomirabilis oxyfera," a Novel Polygon-Shaped Bacterium”. Journal of Bacteriology 194.2 (2012): 284-291.
This data publication is cited in the following publications:
This publication cites the following data publications:

14 Citations in Europe PMC

Data provided by Europe PubMed Central.

The role of paraffin oil on the interaction between denitrifying anaerobic methane oxidation and Anammox processes.
Fu L, Ding ZW, Ding J, Zhang F, Zeng RJ., Appl. Microbiol. Biotechnol. 99(19), 2015
PMID: 26036704
Cytochromes c in Archaea: distribution, maturation, cell architecture, and the special case of Ignicoccus hospitalis.
Kletzin A, Heimerl T, Flechsler J, van Niftrik L, Rachel R, Klingl A., Front Microbiol 6(), 2015
PMID: 26029183
Microbial community composition and ultrastructure of granules from a full-scale anammox reactor.
Gonzalez-Gil G, Sougrat R, Behzad AR, Lens PN, Saikaly PE., Microb. Ecol. 70(1), 2015
PMID: 25501888
XoxF-type methanol dehydrogenase from the anaerobic methanotroph “Candidatus Methylomirabilis oxyfera”.
Wu ML, Wessels JC, Pol A, Op den Camp HJ, Jetten MS, van Niftrik L., Appl. Environ. Microbiol. 81(4), 2015
PMID: 25527536
Anaerobic oxidation of methane: an "active" microbial process.
Cui M, Ma A, Qi H, Zhuang X, Zhuang G., Microbiologyopen 4(1), 2015
PMID: 25530008
Effect of inoculum sources on the enrichment of nitrite-dependent anaerobic methane-oxidizing bacteria.
He Z, Cai C, Shen L, Lou L, Zheng P, Xu X, Hu B., Appl. Microbiol. Biotechnol. 99(2), 2015
PMID: 25186148
Expanding the verrucomicrobial methanotrophic world: description of three novel species of Methylacidimicrobium gen. nov.
van Teeseling MC, Pol A, Harhangi HR, van der Zwart S, Jetten MS, Op den Camp HJ, van Niftrik L., Appl. Environ. Microbiol. 80(21), 2014
PMID: 25172849
High abundance and diversity of nitrite-dependent anaerobic methane-oxidizing bacteria in a paddy field profile.
Zhou L, Wang Y, Long XE, Guo J, Zhu G., FEMS Microbiol. Lett. 360(1), 2014
PMID: 25109910
Autotrophic carbon dioxide fixation via the Calvin-Benson-Bassham cycle by the denitrifying methanotroph "Candidatus Methylomirabilis oxyfera".
Rasigraf O, Kool DM, Jetten MS, Sinninghe Damste JS, Ettwig KF., Appl. Environ. Microbiol. 80(8), 2014
PMID: 24509918
A new addition to the cell plan of anammox bacteria: "Candidatus Kuenenia stuttgartiensis" has a protein surface layer as the outermost layer of the cell.
van Teeseling MC, de Almeida NM, Klingl A, Speth DR, Op den Camp HJ, Rachel R, Jetten MS, van Niftrik L., J. Bacteriol. 196(1), 2014
PMID: 24142254
Methane, microbes and models: fundamental understanding of the soil methane cycle for future predictions.
Nazaries L, Murrell JC, Millard P, Baggs L, Singh BK., Environ. Microbiol. 15(9), 2013
PMID: 23718889
Microbiology, ecology, and application of the nitrite-dependent anaerobic methane oxidation process.
Shen LD, He ZF, Zhu Q, Chen DQ, Lou LP, Xu XY, Zheng P, Hu BL., Front Microbiol 3(), 2012
PMID: 22905032
Co-localization of particulate methane monooxygenase and cd1 nitrite reductase in the denitrifying methanotroph 'Candidatus Methylomirabilis oxyfera'.
Wu ML, van Alen TA, van Donselaar EG, Strous M, Jetten MS, van Niftrik L., FEMS Microbiol. Lett. 334(1), 2012
PMID: 22681179

41 References

Data provided by Europe PubMed Central.

A microbial consortium couples anaerobic methane oxidation to denitrification.
Raghoebarsing AA, Pol A, van de Pas-Schoonen KT, Smolders AJ, Ettwig KF, Rijpstra WI, Schouten S, Damste JS, Op den Camp HJ, Jetten MS, Strous M., Nature 440(7086), 2006
PMID: 16612380
The uncultured microbial majority.
Rappe MS, Giovannoni SJ., Annu. Rev. Microbiol. 57(), 2003
PMID: 14527284
Methane oxidation by cell-free extracts of Methylococcus capsulatus.
Ribbons DW, Michalover JL., FEBS Lett. 11(1), 1970
PMID: 11945445
I. Basic and applied S-layer research: an overview
Sleytr UB., 1997
Bacterial S-layers.
Sleytr UB, Beveridge TJ., Trends Microbiol. 7(6), 1999
PMID: 10366863
S-layers on cell walls of cyanobacteria.
Smarda J, Smajs D, Komrska J, Krzyzanek V., Micron 33(3), 2002
PMID: 11742749
Uber bakterien, welche methan ab knhlenstoffnahrung and energioquelle gebrauchen
Sohngen NL., 1906
Metabolic aspects of aerobic obligate methanotrophy
Trotsenko YA, Murrell JC, Allen SS, Laskin I, Geoffrey MG., 2008
Linking ultrastructure and function in four genera of anaerobic ammonium-oxidizing bacteria: cell plan, glycogen storage, and localization of cytochrome C proteins.
van Niftrik L, Geerts WJ, van Donselaar EG, Humbel BM, Webb RI, Fuerst JA, Verkleij AJ, Jetten MS, Strous M., J. Bacteriol. 190(2), 2008
PMID: 17993524
Square bacterium
Walsby AE., 1980
Physiological role of the respiratory quinol oxidase in the anaerobic nitrite-reducing methanotroph 'Candidatus Methylomirabilis oxyfera'.
Wu ML, de Vries S, van Alen TA, Butler MK, Op den Camp HJ, Keltjens JT, Jetten MS, Strous M., Microbiology (Reading, Engl.) 157(Pt 3), 2011
PMID: 21071492
A new intra-aerobic metabolism in the nitrite-dependent anaerobic methane-oxidizing bacterium Candidatus 'Methylomirabilis oxyfera'.
Wu ML, Ettwig KF, Jetten MS, Strous M, Keltjens JT, van Niftrik L., Biochem. Soc. Trans. 39(1), 2011
PMID: 21265781
Bacterial shape.
Young KD., Mol. Microbiol. 49(3), 2003
PMID: 12914007

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 22020652
PubMed | Europe PMC

Search this title in

Google Scholar