Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells

Mazur L, Opydo-Chanek M, Wojcieszek K, Stojak M, Niemeyer, IIT-GmbH U (2012)
Folia Biologica 60(1): 35-40.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Mafosfamide cyclohexylamine salt (D-17272), 4-hydro-peroxy-cyclophosphamide (D-18864), and beta-D-glucose-isophosphoramide mustard (D-I 9575, glufosfamide) are three new generation oxazaphosphorine agents. The aim of the present study was to compare the cell response to the action of these three oxazaphosphorines. The experiments were performed in vitro on human acute myeloblastic leukemia ML-1 cells. After exposure of ML-1 cells to the oxazaphosphorines, the size, viability and count of these cells were determined. The research was conducted using the spectrophotometric MTT assay and the electronic Beckman Coulter method. The temporary changes in the ML-1 cell size, viability and count, were dependent on the oxazaphosphorine agent tested, its dose, and the time intervals after its application. Among the three oxazaphosphorine agents, D-18864 proved to be the most cytotoxic, and D-19575 was characterized by the lowest cytotoxicity. The results suggest the possibility of using the electronic sizing and counting method and the MTT assay as a rapid in vitro test for assessing leukemic cell sensitivity to the action of new potential chemotherapeutic agents.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Mazur L, Opydo-Chanek M, Wojcieszek K, Stojak M, Niemeyer, IIT-GmbH U. Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica. 2012;60(1):35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., & Niemeyer, IIT-GmbH, U. (2012). Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica, 60(1), 35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., and Niemeyer, IIT-GmbH, U. (2012). Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica 60, 35-40.
Mazur, L., et al., 2012. Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica, 60(1), p 35-40.
L. Mazur, et al., “Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells”, Folia Biologica, vol. 60, 2012, pp. 35-40.
Mazur, L., Opydo-Chanek, M., Wojcieszek, K., Stojak, M., Niemeyer, IIT-GmbH, U.: Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells. Folia Biologica. 60, 35-40 (2012).
Mazur, Lidia, Opydo-Chanek, Malgorzata, Wojcieszek, Katarzyna, Stojak, Marta, and Niemeyer, IIT-GmbH, Ulf. “Comparative Effects of New Generation Oxazaphosphorines on the Size and Viability of Human Acute Myeloblastic Leukemia Cells”. Folia Biologica 60.1 (2012): 35-40.
This data publication is cited in the following publications:
This publication cites the following data publications:

1 Citation in Europe PMC

Data provided by Europe PubMed Central.

The chemomodulatory effects of glufosfamide on docetaxel cytotoxicity in prostate cancer cells.
Attia RT, Tolba MF, Trivedi R, Tadros MG, Arafa HM, Abdel-Naim AB., PeerJ 4(), 2016
PMID: 27413637

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 22428304
PubMed | Europe PMC

Search this title in

Google Scholar