Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals

Oelze M-L, Vogel MO, Alsharafa K, Kahmann U, Viehhauser A, Maurino VG, Dietz K-J (2012)
Journal of Experimental Botany 63(3): 1297-1313.

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Abstract
Chloroplasts are equipped with a nuclear-encoded antioxidant defence system the components of which are usually expressed at high transcript and activity levels. To significantly challenge the chloroplast antioxidant system, Arabidopsis thaliana plants, acclimated to extremely low light slightly above the light compensation point or to normal growth chamber light, were moved to high light corresponding to a 100- and 10-fold light jump, for 6 h and 24 h in order to observe the responses of the water-water cycle at the transcript, protein, enzyme activity, and metabolite levels. The plants coped efficiently with the high light regime and the photoinhibition was fully reversible. Reactive oxygen species (ROS), glutathione and ascorbate levels as well as redox states, respectively, revealed no particular oxidative stress in low-light-acclimated plants transferred to 100-fold excess light. Strong regulation of the water-water cycle enzymes at the transcript level was only partly reflected at the protein and activity levels. In general, low light plants had higher stromal (sAPX) and thylakoid ascorbate peroxidase (tAPX), dehydroascorbate reductase (DHAR), and CuZn superoxide dismutase (CuZnSOD) protein contents than normal light-grown plants. Mutants defective in components relevant for retrograde signalling, namely stn7, ex1, tpt1, and a mutant expressing E .coli catalase in the chloroplast showed unaltered transcriptional responses of water-water cycle enzymes. These findings, together with the response of marker transcripts, indicate that abscisic acid is not involved and that the plastoquinone redox state and reactive oxygen species do not play a major role in regulating the transcriptional response at t=6 h, while other marker transcripts suggest a major role for reductive power, metabolites, and lipids as signals for the response of the water-water cycle.
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Oelze M-L, Vogel MO, Alsharafa K, et al. Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals. Journal of Experimental Botany. 2012;63(3):1297-1313.
Oelze, M. - L., Vogel, M. O., Alsharafa, K., Kahmann, U., Viehhauser, A., Maurino, V. G., & Dietz, K. - J. (2012). Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals. Journal of Experimental Botany, 63(3), 1297-1313.
Oelze, M. - L., Vogel, M. O., Alsharafa, K., Kahmann, U., Viehhauser, A., Maurino, V. G., and Dietz, K. - J. (2012). Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals. Journal of Experimental Botany 63, 1297-1313.
Oelze, M.-L., et al., 2012. Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals. Journal of Experimental Botany, 63(3), p 1297-1313.
M.-L. Oelze, et al., “Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals”, Journal of Experimental Botany, vol. 63, 2012, pp. 1297-1313.
Oelze, M.-L., Vogel, M.O., Alsharafa, K., Kahmann, U., Viehhauser, A., Maurino, V.G., Dietz, K.-J.: Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals. Journal of Experimental Botany. 63, 1297-1313 (2012).
Oelze, Marie-Luise, Vogel, Marc Oliver, Alsharafa, Khalid, Kahmann, Uwe, Viehhauser, Andrea, Maurino, Veronica G, and Dietz, Karl-Josef. “Efficient acclimation of the chloroplast antioxidant defence of Arabidopsis thaliana leaves in response to a 10- or 100-fold light increment and the possible involvement of retrograde signals”. Journal of Experimental Botany 63.3 (2012): 1297-1313.
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Glaßer C, Haberer G, Finkemeier I, Pfannschmidt T, Kleine T, Leister D, Dietz KJ, Hausler RE, Grimm B, Mayer KF., Mol Plant 7(7), 2014
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Alsharafa K, Vogel MO, Oelze ML, Moore M, Stingl N, Konig K, Friedman H, Mueller MJ, Dietz KJ., Philos. Trans. R. Soc. Lond., B, Biol. Sci. 369(1640), 2014
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Light intensity-dependent retrograde signalling in higher plants.
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Retrograde signals galore.
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81 References

Data provided by Europe PubMed Central.

Regulation of gene expression by photosynthetic signals triggered through modified CO2 availability.
Wormuth D, Baier M, Kandlbinder A, Scheibe R, Hartung W, Dietz KJ., BMC Plant Biol. 6(), 2006
PMID: 16916444
Thylakoid membrane-bound ascorbate peroxidase is a limiting factor of antioxidative systems under photo-oxidative stress.
Yabuta Y, Motoki T, Yoshimura K, Takeda T, Ishikawa T, Shigeoka S., Plant J. 32(6), 2002
PMID: 12492834
Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses.
Yoshimura K, Yabuta Y, Ishikawa T, Shigeoka S., Plant Physiol. 123(1), 2000
PMID: 10806239

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