Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection

Chalupowicz L, Zellermann E-M, Flügel M, Dror O, Eichenlaub R, Gartemann K-H, Savidor A, Sessa G, Raki N, Barash I, Manulis-Sasson S (2012)
Phytopathology 102(1): 23-31.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ;
Abstract
Chalupowicz, L., Zellermann, E.-M., Fluegel, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Savidor, A., Sessa, G., Iraki, N., Barash, I., and Manulis-Sasson, S. 2012. Colonization and movement of GFP-labeled Clavibacter michiganensis subsp. michiganensis during tomato infection. Phytopathology 102:23-31. The vascular pathogen Clavibacter michiganensis subsp. michiganensis is responsible for bacterial wilt and canker of tomato. Pathogenicity of this bacterium is dependent on plasmid-borne virulence factors and serine proteases located on the chromosomal chp/tomA pathogenicity island (PAI). In this study, colonization patterns and movement of C.;michiganensis subsp. michiganensis during tomato infection was examined using a green fluorescent protein (GFP)-labeled strain. A plasmid expressing GFP in C. michiganensis subsp. michiganensis was constructed and found to be stable in planta for at least 1 month. Confocal laser-scanning microscopy (CLSM) of inoculated stems showed that the pathogen extensively colonizes the lumen of xylem vessels and preferentially attaches to spiral secondary wall thickening of the protoxylem. Acropetal movement of the wild-type strain C. michiganensis subsp. michiganensis NCPPB382 (Cmm382) in tomato resulted in an extensive systemic colonization of the whole plant reaching the apical region after 15 days, whereas Cmm100 (lacking the plasmids pCM1 and pCM2) or Cmm27 (lacking the chp/tomA PAI) remained confined to the area surrounding of the inoculation site. Cmm382 formed biofilm-like structures composed of large bacterial aggregates on the interior of xylem walls as observed by CLSM and scanning electron microscopy. These findings suggest that virulence factors located on the chp/tomA PAI or the plasmids are required for effective movement of the pathogen in tomato and for the formation of cellular aggregates.
Publishing Year
ISSN
PUB-ID

Cite this

Chalupowicz L, Zellermann E-M, Flügel M, et al. Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection. Phytopathology. 2012;102(1):23-31.
Chalupowicz, L., Zellermann, E. - M., Flügel, M., Dror, O., Eichenlaub, R., Gartemann, K. - H., Savidor, A., et al. (2012). Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection. Phytopathology, 102(1), 23-31.
Chalupowicz, L., Zellermann, E. - M., Flügel, M., Dror, O., Eichenlaub, R., Gartemann, K. - H., Savidor, A., Sessa, G., Raki, N., Barash, I., et al. (2012). Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection. Phytopathology 102, 23-31.
Chalupowicz, L., et al., 2012. Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection. Phytopathology, 102(1), p 23-31.
L. Chalupowicz, et al., “Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection”, Phytopathology, vol. 102, 2012, pp. 23-31.
Chalupowicz, L., Zellermann, E.-M., Flügel, M., Dror, O., Eichenlaub, R., Gartemann, K.-H., Savidor, A., Sessa, G., Raki, N., Barash, I., Manulis-Sasson, S.: Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection. Phytopathology. 102, 23-31 (2012).
Chalupowicz, L., Zellermann, Eva-Maria, Flügel, Monika, Dror, O., Eichenlaub, Rudolf, Gartemann, Karl-Heinz, Savidor, A., Sessa, G., Raki, N., Barash, I., and Manulis-Sasson, S. “Colonization and Movement of GFP-Labeled Clavibacter michiganensis subsp michiganensis During Tomato Infection”. Phytopathology 102.1 (2012): 23-31.
This data publication is cited in the following publications:
This publication cites the following data publications:

7 Citations in Europe PMC

Data provided by Europe PubMed Central.

How way leads on to way.
Barash I., Annu Rev Phytopathol 52(), 2014
PMID: 25090476
Studies on the colonization of axenically grown tomato plants by a GFP-tagged strain of Clavibacter michiganensis subsp. michiganensis
Lelis FMV, Czajkowski R, de Souza RM, Ribeiro DH, van der Wolf JM., Eur. J. Plant Pathol. 139(1), 2014
PMID: IND500743147
Tomato fruit and seed colonization by Clavibacter michiganensis subsp. michiganensis through external and internal routes.
Tancos MA, Chalupowicz L, Barash I, Manulis-Sasson S, Smart CD., Appl. Environ. Microbiol. 79(22), 2013
PMID: 24014525
Colonization patterns of an mCherry-tagged Pectobacterium carotovorum subsp. brasiliense strain in potato plants.
Kubheka GC, Coutinho TA, Moleleki N, Moleleki LN., Phytopathology 103(12), 2013
PMID: 23758294
Cyclic Di-GMP modulates the disease progression of Erwinia amylovora.
Edmunds AC, Castiblanco LF, Sundin GW, Waters CM., J. Bacteriol. 195(10), 2013
PMID: 23475975

32 References

Data provided by Europe PubMed Central.

Biofilm formation in plant-microbe associations.
Ramey BE, Koutsoudis M, von Bodman SB, Fuqua C., Curr. Opin. Microbiol. 7(6), 2004
PMID: 15556032

Stumpp, BIOspektrum (), 2000
Green fluorescent protein as a reporter for spatial and temporal gene expression in Streptomyces coelicolor A3(2).
Sun J, Kelemen GH, Fernandez-Abalos JM, Bibb MJ., Microbiology (Reading, Engl.) 145 ( Pt 9)(), 1999
PMID: 10517575
Quorum sensing in plant-pathogenic bacteria.
Von Bodman SB, Bauer WD, Coplin DL., Annu Rev Phytopathol 41(), 2003
PMID: 12730390

AUTHOR UNKNOWN, 0
Bioluminescence imaging of Clavibacter michiganensis subsp. michiganensis infection of tomato seeds and plants.
Xu X, Miller SA, Baysal-Gurel F, Gartemann KH, Eichenlaub R, Rajashekara G., Appl. Environ. Microbiol. 76(12), 2010
PMID: 20400561

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 21879791
PubMed | Europe PMC

Search this title in

Google Scholar