Superresolution Optical Fluctuation Imaging (SOFI)

Dertinger T, Coyler R, Vogel R, Heilemann M, Sauer M, Enderlein J, Weiss S (2012)
In: Nano-Biotechnology for Biomedical and Diagnostic Research. Zahavy E, Ordentlich A, Yitzhaki S, Shafferman A (Eds); Advances in Experimental Medicine and Biology, 733. Springer: 17-21.

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Zahavy, Eran ; Ordentlich, Arie ; Yitzhaki, Shmuel ; Shafferman, Avigdor
Abstract
Superresolution microscopy has shifted the limits for fluorescence microscopy in cell ­biology. The possibility to image cellular structures and dynamics of fixed and even live cells and organisms at resolutions of several nanometers holds great promise for future biological discoveries. We recently introduced a novel superresolution technique, based on the statistical evaluation of stochastic fluctuations stemming from single emitters, dubbed "superresolution optical fluctuation ­imaging" (SOFI). In comparison to previously introduced superresolution methods, SOFI exhibits favorable attributes such as simplicity, affordability, high speed, and low levels of light exposure. Here we summarize the basic working principle and recent advances.
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Dertinger T, Coyler R, Vogel R, et al. Superresolution Optical Fluctuation Imaging (SOFI). In: Zahavy E, Ordentlich A, Yitzhaki S, Shafferman A, eds. Nano-Biotechnology for Biomedical and Diagnostic Research. Advances in Experimental Medicine and Biology. Vol 733. Springer; 2012: 17-21.
Dertinger, T., Coyler, R., Vogel, R., Heilemann, M., Sauer, M., Enderlein, J., & Weiss, S. (2012). Superresolution Optical Fluctuation Imaging (SOFI). In E. Zahavy, A. Ordentlich, S. Yitzhaki, & A. Shafferman (Eds.), Advances in Experimental Medicine and Biology: Vol. 733. Nano-Biotechnology for Biomedical and Diagnostic Research (pp. 17-21). Springer.
Dertinger, T., Coyler, R., Vogel, R., Heilemann, M., Sauer, M., Enderlein, J., and Weiss, S. (2012). “Superresolution Optical Fluctuation Imaging (SOFI)” in Nano-Biotechnology for Biomedical and Diagnostic Research, ed. E. Zahavy, A. Ordentlich, S. Yitzhaki, and A. Shafferman Advances in Experimental Medicine and Biology, vol. 733, (Springer), 17-21.
Dertinger, T., et al., 2012. Superresolution Optical Fluctuation Imaging (SOFI). In E. Zahavy, et al., eds. Nano-Biotechnology for Biomedical and Diagnostic Research. Advances in Experimental Medicine and Biology. no.733 Springer, pp. 17-21.
T. Dertinger, et al., “Superresolution Optical Fluctuation Imaging (SOFI)”, Nano-Biotechnology for Biomedical and Diagnostic Research, E. Zahavy, et al., eds., Advances in Experimental Medicine and Biology, vol. 733, Springer, 2012, pp.17-21.
Dertinger, T., Coyler, R., Vogel, R., Heilemann, M., Sauer, M., Enderlein, J., Weiss, S.: Superresolution Optical Fluctuation Imaging (SOFI). In: Zahavy, E., Ordentlich, A., Yitzhaki, S., and Shafferman, A. (eds.) Nano-Biotechnology for Biomedical and Diagnostic Research. Advances in Experimental Medicine and Biology. 733, p. 17-21. Springer (2012).
Dertinger, Thomas, Coyler, Ryan, Vogel, Robert, Heilemann, Mike, Sauer, Markus, Enderlein, Jörg, and Weiss, Shimon. “Superresolution Optical Fluctuation Imaging (SOFI)”. Nano-Biotechnology for Biomedical and Diagnostic Research. Ed. Eran Zahavy, Arie Ordentlich, Shmuel Yitzhaki, and Avigdor Shafferman. Springer, 2012.Vol. 733. Advances in Experimental Medicine and Biology. 17-21.
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