Involvement of CD44v6 in InlB-dependent Listeria invasion

Jung C, Matzke A, Niemann H, Schwerk C, Tenenbaum T, Orian-Rousseau V (2009)
Molecular Microbiology 72(5): 1196-1207.

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Listeria monocytogenes, a Gram-positive bacterium, is the causative agent for the disease called listeriosis. This pathogen utilizes host cell surface proteins such as E-cadherin or c-Met in order to invade eukaryotic cells. The invasion via c-Met depends on the bacterial protein InlB that activates c-Met phosphorylation and internalization mimicking in many regards HGF, the authentic c-Met ligand. In this paper, we demonstrate that the activation of c-Met induced by InlB is dependent on CD44v6, a member of the CD44 family of transmembrane glycoproteins. Inhibiting CD44v6 by means of a blocking peptide, a CD44v6 antibody or CD44v6-specific siRNA prevents the activation of c-Met induced by InlB. Subsequently, signalling, scattering and the entry of InlB-coated beads into host cells are also impaired by CD44v6 blocking reagents. For the entry process, ezrin, a protein that links the CD44v6 cytoplasmic domain to the cytoskeleton, is required as well. Most importantly, this collaboration between c-Met and CD44v6 contributes to the invasion of L. monocytogenes into target cells as demonstrated by a drastic decrease in bacterial invasion in the presence of blocking agents such as the CD44v6 peptide or antibody.
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Jung C, Matzke A, Niemann H, Schwerk C, Tenenbaum T, Orian-Rousseau V. Involvement of CD44v6 in InlB-dependent Listeria invasion. Molecular Microbiology. 2009;72(5):1196-1207.
Jung, C., Matzke, A., Niemann, H., Schwerk, C., Tenenbaum, T., & Orian-Rousseau, V. (2009). Involvement of CD44v6 in InlB-dependent Listeria invasion. Molecular Microbiology, 72(5), 1196-1207.
Jung, C., Matzke, A., Niemann, H., Schwerk, C., Tenenbaum, T., and Orian-Rousseau, V. (2009). Involvement of CD44v6 in InlB-dependent Listeria invasion. Molecular Microbiology 72, 1196-1207.
Jung, C., et al., 2009. Involvement of CD44v6 in InlB-dependent Listeria invasion. Molecular Microbiology, 72(5), p 1196-1207.
C. Jung, et al., “Involvement of CD44v6 in InlB-dependent Listeria invasion”, Molecular Microbiology, vol. 72, 2009, pp. 1196-1207.
Jung, C., Matzke, A., Niemann, H., Schwerk, C., Tenenbaum, T., Orian-Rousseau, V.: Involvement of CD44v6 in InlB-dependent Listeria invasion. Molecular Microbiology. 72, 1196-1207 (2009).
Jung, C., Matzke, A., Niemann, Hartmut, Schwerk, Christian, Tenenbaum, Tobias, and Orian-Rousseau, V. “Involvement of CD44v6 in InlB-dependent Listeria invasion”. Molecular Microbiology 72.5 (2009): 1196-1207.
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