RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System

Schöning JC, Staiger D (2009)
In: Plant Signal Transduction. Pfannschmidt T (Ed); Methods in Molecular Biology, 479. Humana Press: 337-351.

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Pfannschmidt, Th.
Abstract
Post-transcriptional control makes an important contribution to shaping transcript profiles of circadianly regulated genes. In Arabidopsis thaliana , the clock-regulated glycine-rich RNA-binding protein AT GRP7 oscillates with a 24-h rhythm and transmits the rhythmicity generated by the central oscillator within the cell. AT GRP7 negatively auto-regulates its own expression at the post-transcriptional level. In response to an elevated protein level, a shift to a cryptic 5′ splice site within the intron occurs, leading to an unproductively spliced transcript that rapidly vanishes due to its short half-life. This feedback regulation relies on direct binding of the RNA-binding protein to its own RNA. Here we describe the analysis of RNA-protein interaction in vitro employing recombinant RNA-binding protein and 32 P-labelled in vitro transcripts or synthetic RNA oligoribonucleotides comprising the binding site under study.
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Schöning JC, Staiger D. RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System. In: Pfannschmidt T, ed. Plant Signal Transduction. Methods in Molecular Biology. Vol 479. Humana Press; 2009: 337-351.
Schöning, J. C., & Staiger, D. (2009). RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System. In T. Pfannschmidt (Ed.), Methods in Molecular Biology: Vol. 479. Plant Signal Transduction (pp. 337-351). Humana Press.
Schöning, J. C., and Staiger, D. (2009). “RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System” in Plant Signal Transduction, ed. T. Pfannschmidt Methods in Molecular Biology, vol. 479, (Humana Press), 337-351.
Schöning, J.C., & Staiger, D., 2009. RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System. In T. Pfannschmidt, ed. Plant Signal Transduction. Methods in Molecular Biology. no.479 Humana Press, pp. 337-351.
J.C. Schöning and D. Staiger, “RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System”, Plant Signal Transduction, T. Pfannschmidt, ed., Methods in Molecular Biology, vol. 479, Humana Press, 2009, pp.337-351.
Schöning, J.C., Staiger, D.: RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System. In: Pfannschmidt, T. (ed.) Plant Signal Transduction. Methods in Molecular Biology. 479, p. 337-351. Humana Press (2009).
Schöning, Jan C., and Staiger, Dorothee. “RNA-protein Interaction Mediating Post-transcriptional Regulation in the Circadian System”. Plant Signal Transduction. Ed. Th. Pfannschmidt. Humana Press, 2009.Vol. 479. Methods in Molecular Biology. 337-351.
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Quantitative analysis of single-molecule RNA-protein interaction.
Fuhrmann A, Schoening JC, Anselmetti D, Staiger D, Ros R., Biophys. J. 96(12), 2009
PMID: 19527663

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