Tuber-specific cphA expression to enhance cyanophycin production in potatoes

Hühns M, Neumann K, Hausmann T, Klemke F, Lockau W, Kahmann U, Kopertekh L, Staiger D, Pistorius EK, Reuther J, Waldvogel E, et al. (2009)
Plant Biotechnology Journal 7(9): 883-898.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract
The production of biodegradable polymers that can be used to substitute petrochemical compounds in commercial products in transgenic plants is an important challenge for plant biotechnology. Nevertheless, it is often accompanied by reduced plant fitness. To decrease the phenotypic abnormalities of the sprout and to increase polymer production, we restricted cyanophycin accumulation to the potato tubers by using the cyanophycin synthetase gene (cphATe) from Thermosynechococcus elongatus BP-1, which is under the control of the tuber-specific class 1 promoter (B33). Tuber-specific cytosolic (pB33-cphATe) as well as tuber-specific plastidic (pB33-PsbY-cphATe) expression resulted in significant polymer accumulation solely in the tubers. In plants transformed with pB33-cphATe, both cyanophycin synthetase and cyanophycin were detected in the cytoplasm leading to an increase up to 2.3% cyanophycin of dry weight and resulting in small and deformed tubers. In B33-PsbY-cphATe tubers, cyanophycin synthetase and cyanophycin were exclusively found in amyloplasts leading to a cyanophycin accumulation up to 7.5% of dry weight. These tubers were normal in size, some clones showed reduced tuber yield and sometimes exhibited brown sunken staining starting at tubers navel. During a storage period over of 32 weeks of one selected clone, the cyanophycin content was stable in B33-PsbY-cphATe tubers but the stress symptoms increased. However, all tubers were able to germinate. Nitrogen fertilization in the greenhouse led not to an increased cyanophycin yield, slightly reduced protein content, decreased starch content, and changes in the amounts of bound and free arginine and aspartate, as compared with control tubers were observed.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Hühns M, Neumann K, Hausmann T, et al. Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnology Journal. 2009;7(9):883-898.
Hühns, M., Neumann, K., Hausmann, T., Klemke, F., Lockau, W., Kahmann, U., Kopertekh, L., et al. (2009). Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnology Journal, 7(9), 883-898.
Hühns, M., Neumann, K., Hausmann, T., Klemke, F., Lockau, W., Kahmann, U., Kopertekh, L., Staiger, D., Pistorius, E. K., Reuther, J., et al. (2009). Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnology Journal 7, 883-898.
Hühns, M., et al., 2009. Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnology Journal, 7(9), p 883-898.
M. Hühns, et al., “Tuber-specific cphA expression to enhance cyanophycin production in potatoes”, Plant Biotechnology Journal, vol. 7, 2009, pp. 883-898.
Hühns, M., Neumann, K., Hausmann, T., Klemke, F., Lockau, W., Kahmann, U., Kopertekh, L., Staiger, D., Pistorius, E.K., Reuther, J., Waldvogel, E., Wohlleben, W., Effmert, M., Junghans, H., Neubauer, K., Kragl, U., Schmidt, K., Schmidtke, J., Broer, I.: Tuber-specific cphA expression to enhance cyanophycin production in potatoes. Plant Biotechnology Journal. 7, 883-898 (2009).
Hühns, Maja, Neumann, Katrin, Hausmann, Tina, Klemke, Friederike, Lockau, Wolfgang, Kahmann, Uwe, Kopertekh, Lilya, Staiger, Dorothee, Pistorius, Elfriede K., Reuther, Jens, Waldvogel, Eva, Wohlleben, Wolfgang, Effmert, Martin, Junghans, Holger, Neubauer, Katja, Kragl, Udo, Schmidt, Kerstin, Schmidtke, Jörg, and Broer, Inge. “Tuber-specific cphA expression to enhance cyanophycin production in potatoes”. Plant Biotechnology Journal 7.9 (2009): 883-898.
This data publication is cited in the following publications:
This publication cites the following data publications:

3 Citations in Europe PMC

Data provided by Europe PubMed Central.

Plastid genetic engineering in Solanaceae.
Venkatesh J, Park SW., Protoplasma 249(4), 2012
PMID: 22395455
Greenhouse and field cultivations of antigen-expressing potatoes focusing on the variability in plant constituents and antigen expression.
Mikschofsky H, Heilmann E, Schmidtke J, Schmidt K, Meyer U, Leinweber P, Broer I., Plant Mol. Biol. 76(1-2), 2011
PMID: 21594687
Heterologous expression of Anabaena sp. PCC7120 cyanophycin metabolism genes cphA1 and cphB1 in Sinorhizobium (Ensifer) meliloti 1021.
Abd-El-Karem Y, Elbers T, Reichelt R, Steinbuchel A., Appl. Microbiol. Biotechnol. 89(4), 2011
PMID: 20938772

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 19843250
PubMed | Europe PMC

Search this title in

Google Scholar