Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves

BETZ M, MARTINOIA E, HINCHA DK, SCHMITT JM, Dietz K-J (1992)
Phytochemistry 31(2): 433-440.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
Apoplastic and vacuolar forms of alpha-mannosidase from barley leaves were purified (the latter ca 1000-fold). Isoenzymes were separated during purification. They consisted of four subunits with an apparent M(r) of 53, 46, 40 and 24 x 10(3) in varying proportions. In two-dimensional electropherograms, the M(r) 46 and 40 x 10(3) polypeptides were separated into two isomeric forms of different isoelectric point. The native proteins focused in a pH gradient at different isoelectric points. Their M(r)S ranged between 290 and 270 x 10(3) as judged from size exclusion chromatography and electrophoretic mobility. The compartmentation of the isoenzymes revealed tissue- and age-specificity.
Erscheinungsjahr
Zeitschriftentitel
Phytochemistry
Band
31
Zeitschriftennummer
2
Seite
433-440
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PUB-ID

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BETZ M, MARTINOIA E, HINCHA DK, SCHMITT JM, Dietz K-J. Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry. 1992;31(2):433-440.
BETZ, M., MARTINOIA, E., HINCHA, D. K., SCHMITT, J. M., & Dietz, K. - J. (1992). Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry, 31(2), 433-440. doi:10.1016/0031-9422(92)90012-F
BETZ, M., MARTINOIA, E., HINCHA, D. K., SCHMITT, J. M., and Dietz, K. - J. (1992). Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry 31, 433-440.
BETZ, M., et al., 1992. Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry, 31(2), p 433-440.
M. BETZ, et al., “Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves”, Phytochemistry, vol. 31, 1992, pp. 433-440.
BETZ, M., MARTINOIA, E., HINCHA, D.K., SCHMITT, J.M., Dietz, K.-J.: Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry. 31, 433-440 (1992).
BETZ, M, MARTINOIA, E, HINCHA, DK, SCHMITT, JM, and Dietz, Karl-Josef. “Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves”. Phytochemistry 31.2 (1992): 433-440.