Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves

BETZ M, MARTINOIA E, HINCHA DK, SCHMITT JM, Dietz K-J (1992)
Phytochemistry 31(2): 433-440.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ; ;
Abstract
Apoplastic and vacuolar forms of alpha-mannosidase from barley leaves were purified (the latter ca 1000-fold). Isoenzymes were separated during purification. They consisted of four subunits with an apparent M(r) of 53, 46, 40 and 24 x 10(3) in varying proportions. In two-dimensional electropherograms, the M(r) 46 and 40 x 10(3) polypeptides were separated into two isomeric forms of different isoelectric point. The native proteins focused in a pH gradient at different isoelectric points. Their M(r)S ranged between 290 and 270 x 10(3) as judged from size exclusion chromatography and electrophoretic mobility. The compartmentation of the isoenzymes revealed tissue- and age-specificity.
Publishing Year
ISSN
PUB-ID

Cite this

BETZ M, MARTINOIA E, HINCHA DK, SCHMITT JM, Dietz K-J. Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry. 1992;31(2):433-440.
BETZ, M., MARTINOIA, E., HINCHA, D. K., SCHMITT, J. M., & Dietz, K. - J. (1992). Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry, 31(2), 433-440.
BETZ, M., MARTINOIA, E., HINCHA, D. K., SCHMITT, J. M., and Dietz, K. - J. (1992). Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry 31, 433-440.
BETZ, M., et al., 1992. Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry, 31(2), p 433-440.
M. BETZ, et al., “Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves”, Phytochemistry, vol. 31, 1992, pp. 433-440.
BETZ, M., MARTINOIA, E., HINCHA, D.K., SCHMITT, J.M., Dietz, K.-J.: Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves. Phytochemistry. 31, 433-440 (1992).
BETZ, M, MARTINOIA, E, HINCHA, DK, SCHMITT, JM, and Dietz, Karl-Josef. “Purification and compartmentation of alpha-mannosidase isoenzymes of barley leaves”. Phytochemistry 31.2 (1992): 433-440.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Search this title in

Google Scholar