Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel

Klughammer B, Betz M, Benz R, Dietz K-J (1992)
Journal of Membrane Biology 128(1): 17-25.

Journal Article | Published | English

No fulltext has been uploaded

Author
; ; ;
Abstract
A potassium-specific tonoplast channel was identified by reconstitution of tonoplast polypeptides into planar lipid bilayer membranes. Highly purified tonoplast membranes were solubilized in Triton X-100-containing buffer and fractionated by size-exclusion chromatography. The protein fractions were assayed for ion channel activity in a planar bilayer system, and the potassium channel was routinely recovered in specific fractions corresponding to an apparent molecular mass of 80 kDa. In symmetrical electrolyte solutions of 100 mm potassium chloride, the potassium channel had a single-channel conductance of 72 pS. Substates of the channel with conductances of 17, 33 and 52 pS were frequently observed. After identification of the channel in low or high KCl, addition of sodium acetate or sodium chloride caused only insignificant conductance changes. This result suggested that the channel was not or little permeable for sodium or chloride, whereas it had similar single-channel conductance for rubidium and caesium ions as compared with potassium ions. The channel is presumably responsible for the equilibration of potassium between the vacuole and the cytosol. The role of the channel in the physiology of the barley cell under salt stress is discussed.
Publishing Year
ISSN
PUB-ID

Cite this

Klughammer B, Betz M, Benz R, Dietz K-J. Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel. Journal of Membrane Biology. 1992;128(1):17-25.
Klughammer, B., Betz, M., Benz, R., & Dietz, K. - J. (1992). Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel. Journal of Membrane Biology, 128(1), 17-25.
Klughammer, B., Betz, M., Benz, R., and Dietz, K. - J. (1992). Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel. Journal of Membrane Biology 128, 17-25.
Klughammer, B., et al., 1992. Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel. Journal of Membrane Biology, 128(1), p 17-25.
B. Klughammer, et al., “Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel”, Journal of Membrane Biology, vol. 128, 1992, pp. 17-25.
Klughammer, B., Betz, M., Benz, R., Dietz, K.-J.: Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel. Journal of Membrane Biology. 128, 17-25 (1992).
Klughammer, B., Betz, M., Benz, R., and Dietz, Karl-Josef. “Partial purification of a potassium channel with low permeability for sodium from tonoplast membranes of Hordeum vulgare cv. Gerbel”. Journal of Membrane Biology 128.1 (1992): 17-25.
This data publication is cited in the following publications:
This publication cites the following data publications:

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 1501236
PubMed | Europe PMC

Search this title in

Google Scholar