Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis

Dietz K-J, Rudloff S, Ageorges A, Eckerskorn C, Fischer K, Arbinger B (1995)
The Plant Journal 8(4): 521-529.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ;
Abstract
A tonoplast protein of 31 kDa apparent molecular mass (TpP 31) was isolated from two-dimensional gels. Amino acid sequences were determined from LysC endoproteinase-peptide fragments. Using degenerate oligonucleotides, a corresponding cDNA clone of 1034 bp was isolated from a barley leaf cDNA library. It encodes for subunit E of the vacuolar H+-ATPase, the first one identi fled in plants so far. The open reading frame extends over 681 bp, encoding a gene product of 227 amino acids and a calculated molecular weight of 26 228 g mol(-1). Northern and Western blot analysis indicates constitutive expression of subunit E in all plant organs with only small effects of salt stress. Localization of TpP 31 at the tonoplast was confirmed in fractions of purified vacuolar membrane obtained by free-flow electrophoresis. Immunoprecipitation of newly synthesized S-35-labelled membrane proteins with anti-TpP 31 gave two additional bands with apparent molecular masses of about 53 and 62 kDa. Gel filtration after mild solubilization showed co-purification of TpP 31 with the 55 kDa subunit of the H+-ATPase. Both results provide evidence beyond the sequence homology that TpP 31 is a structural component of the vacuolar H+-ATPase.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Dietz K-J, Rudloff S, Ageorges A, Eckerskorn C, Fischer K, Arbinger B. Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. The Plant Journal. 1995;8(4):521-529.
Dietz, K. - J., Rudloff, S., Ageorges, A., Eckerskorn, C., Fischer, K., & Arbinger, B. (1995). Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. The Plant Journal, 8(4), 521-529. doi:10.1046/j.1365-313X.1995.8040521.x
Dietz, K. - J., Rudloff, S., Ageorges, A., Eckerskorn, C., Fischer, K., and Arbinger, B. (1995). Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. The Plant Journal 8, 521-529.
Dietz, K.-J., et al., 1995. Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. The Plant Journal, 8(4), p 521-529.
K.-J. Dietz, et al., “Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis”, The Plant Journal, vol. 8, 1995, pp. 521-529.
Dietz, K.-J., Rudloff, S., Ageorges, A., Eckerskorn, C., Fischer, K., Arbinger, B.: Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis. The Plant Journal. 8, 521-529 (1995).
Dietz, Karl-Josef, Rudloff, S., Ageorges, A., Eckerskorn, C., Fischer, K., and Arbinger, B. “Subunit E of the vacuolar H+-ATPase of Hordeum vulgare L.: cDNA cloning, expression and immunological analysis”. The Plant Journal 8.4 (1995): 521-529.
This data publication is cited in the following publications:
This publication cites the following data publications:

19 Citations in Europe PMC

Data provided by Europe PubMed Central.

Regulation of the V-type ATPase by redox modulation.
Seidel T, Scholl S, Krebs M, Rienmuller F, Marten I, Hedrich R, Hanitzsch M, Janetzki P, Dietz KJ, Schumacher K., Biochem. J. 448(2), 2012
PMID: 22943363
Cloning and functional analysis of wheat V-H+-ATPase subunit genes.
Zhao Q, Zhao YJ, Zhao BC, Ge RC, Li M, Shen YZ, Huang ZJ., Plant Mol. Biol. 69(1-2), 2009
PMID: 18836689
Resistance of wheat to Mycosphaerella graminicola involves early and late peaks of gene expression
Adhikari TB, Balaji B, Breeden J., Physiol. Mol. Plant Pathol. 71(), 2007
PMID: IND500692879
Subcellular distribution of the V-ATPase complex in plant cells, and in vivo localisation of the 100 kDa subunit VHA-a within the complex.
Kluge C, Seidel T, Bolte S, Sharma SS, Hanitzsch M, Satiat-Jeunemaitre B, Ross J, Sauer M, Golldack D, Dietz KJ., BMC Cell Biol. 5(), 2004
PMID: 15310389
Alterations in Cd-induced gene expression under nitrogen deficiency in Hordeum vulgare.
Finkemeier I, Kluge C, Metwally A, Georgi M, Grotjohann N, Dietz KJ., Plant Cell Environ. 26(6), 2003
PMID: 12803610
A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase.
Sze H, Schumacher K, Muller ML, Padmanaban S, Taiz L., Trends Plant Sci. 7(4), 2002
PMID: 11950611
Transport of 1-kestose across the tonoplast of Jerusalem artichoke tubers.
Pontis HG, Gonzalez P, Etxeberria E., Phytochemistry 59(3), 2002
PMID: 11830131
Significance of the V-type ATPase for the adaptation to stressful growth conditions and its regulation on the molecular and biochemical level.
Dietz KJ, Tavakoli N, Kluge C, Mimura T, Sharma SS, Harris GC, Chardonnens AN, Golldack D., J. Exp. Bot. 52(363), 2001
PMID: 11559732
CLC-Nt1, a putative chloride channel protein of tobacco, co-localizes with mitochondrial membrane markers.
Lurin C, Guclu J, Cheniclet C, Carde JP, Barbier-Brygoo H, Maurel C., Biochem. J. 348 Pt 2(), 2000
PMID: 10816421
Membrane proteins and proteomics: un amour impossible?
Santoni V, Molloy M, Rabilloud T., Electrophoresis 21(6), 2000
PMID: 10786880
Structure, function and regulation of the plant vacuolar H(+)-translocating ATPase.
Ratajczak R., Biochim. Biophys. Acta 1465(1-2), 2000
PMID: 10748245
Subunit C of the vacuolar H+-ATPase of Hordeum vulgare.
Tavakoli N, Eckerskorn C, Golldack D, Dietz KJ., FEBS Lett. 456(1), 1999
PMID: 10452532
Cloning of the V-ATPase subunit G in plant: functional expression and sub-cellular localization.
Rouquie D, Tournaire-Roux C, Szponarski W, Rossignol M, Doumas P., FEBS Lett. 437(3), 1998
PMID: 9824309
Down-regulation of plant V-type H+ -ATPase genes after light-induced inhibition of growth.
Viereck R, Kirsch M, Low R, Rausch T., FEBS Lett. 384(3), 1996
PMID: 8617373

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 7496398
PubMed | Europe PMC

Search this title in

Google Scholar