A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development

Baer K, Dückers N, Rosenbaum T, Leggewie C, Simon S, Krausser M, Osswald S, Hummel W, Gröger H (2011)
Tetrahedron Asymmetry 22(9): 925-928.

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Zeitschriftenaufsatz | Veröffentlicht | Englisch
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Abstract / Bemerkung
The development of aldol reactions of glycine with substituted benzaldehydes in the presence of recombinant L-threonine aldolases from Escherichia coil or Saccharomyces cerevisiae, which were obtained with excellent overexpression data, has been carried out. When using glycine and ortho-chlorobenzaldehyde, a high conversion of >95%, an enantioselectivity of >99% ee, and a diastereoselectivity with d.r.(syn/anti) = 80:20 was obtained for the resulting beta-hydroxy alpha-amino acid in such a biotransformation. It should be noted that this enzymatic process can be conducted at an elevated substrate concentration of 250 mM. (C) 2011 Elsevier Ltd. All rights reserved.
Erscheinungsjahr
Zeitschriftentitel
Tetrahedron Asymmetry
Band
22
Zeitschriftennummer
9
Seite
925-928
ISSN
PUB-ID

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Baer K, Dückers N, Rosenbaum T, et al. A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development. Tetrahedron Asymmetry. 2011;22(9):925-928.
Baer, K., Dückers, N., Rosenbaum, T., Leggewie, C., Simon, S., Krausser, M., Osswald, S., et al. (2011). A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development. Tetrahedron Asymmetry, 22(9), 925-928. doi:10.1016/j.tetasy.2011.04.016
Baer, K., Dückers, N., Rosenbaum, T., Leggewie, C., Simon, S., Krausser, M., Osswald, S., Hummel, W., and Gröger, H. (2011). A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development. Tetrahedron Asymmetry 22, 925-928.
Baer, K., et al., 2011. A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development. Tetrahedron Asymmetry, 22(9), p 925-928.
K. Baer, et al., “A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development”, Tetrahedron Asymmetry, vol. 22, 2011, pp. 925-928.
Baer, K., Dückers, N., Rosenbaum, T., Leggewie, C., Simon, S., Krausser, M., Osswald, S., Hummel, W., Gröger, H.: A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development. Tetrahedron Asymmetry. 22, 925-928 (2011).
Baer, Katrin, Dückers, Nina, Rosenbaum, Thorsten, Leggewie, Christian, Simon, Sabine, Krausser, Marina, Osswald, Steffen, Hummel, Werner, and Gröger, Harald. “A study towards efficient L-threonine aldolase-catalyzed enantio- and diastereoselective aldol reactions of glycine with substituted benzaldehydes: biocatalyst production and process development”. Tetrahedron Asymmetry 22.9 (2011): 925-928.