Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis

Hinse D, Vollmer T, Rückert C, Blom J, Kalinowski J, Knabbe C, Dreier J (2011)
BMC Genomics 12(1).

Journal Article | Published | English

No fulltext has been uploaded

Abstract
Background: Streptococcus gallolyticus subsp. gallolyticus is an important causative agent of infectious endocarditis, while the pathogenicity of this species is widely unclear. To gain insight into the pathomechanisms and the underlying genetic elements for lateral gene transfer, we sequenced the entire genome of this pathogen. Results: We sequenced the whole genome of S. gallolyticus subsp. gallolyticus strain ATCC BAA-2069, consisting of a 2,356,444 bp circular DNA molecule with a G+C-content of 37.65% and a novel 20,765 bp plasmid designated as pSGG1. Bioinformatic analysis predicted 2,309 ORFs and the presence of 80 tRNAs and 21 rRNAs in the chromosome. Furthermore, 21 ORFs were detected on the plasmid pSGG1, including tetracycline resistance genes telL and tet(O/W/32/O). Screening of 41 S. gallolyticus subsp. gallolyticus isolates revealed one plasmid (pSGG2) homologous to pSGG1. We further predicted 21 surface proteins containing the cell wall-sorting motif LPxTG, which were shown to play a functional role in the adhesion of bacteria to host cells. In addition, we performed a whole genome comparison to the recently sequenced S. gallolyticus subsp. gallolyticus strain UCN34, revealing significant differences. Conclusions: The analysis of the whole genome sequence of S. gallolyticus subsp. gallolyticus promotes understanding of genetic factors concerning the pathogenesis and adhesion to ECM of this pathogen. For the first time we detected the presence of the mobilizable pSGG1 plasmid, which may play a functional role in lateral gene transfer and promote a selective advantage due to a tetracycline resistance.
Publishing Year
ISSN
PUB-ID

Cite this

Hinse D, Vollmer T, Rückert C, et al. Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis. BMC Genomics. 2011;12(1).
Hinse, D., Vollmer, T., Rückert, C., Blom, J., Kalinowski, J., Knabbe, C., & Dreier, J. (2011). Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis. BMC Genomics, 12(1).
Hinse, D., Vollmer, T., Rückert, C., Blom, J., Kalinowski, J., Knabbe, C., and Dreier, J. (2011). Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis. BMC Genomics 12.
Hinse, D., et al., 2011. Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis. BMC Genomics, 12(1).
D. Hinse, et al., “Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis”, BMC Genomics, vol. 12, 2011.
Hinse, D., Vollmer, T., Rückert, C., Blom, J., Kalinowski, J., Knabbe, C., Dreier, J.: Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis. BMC Genomics. 12, (2011).
Hinse, Dennis, Vollmer, Tanja, Rückert, Christian, Blom, Jochen, Kalinowski, Jörn, Knabbe, Cornelius, and Dreier, Jens. “Complete genome and comparative analysis of Streptococcus gallolyticus subsp gallolyticus, an emerging pathogen of infective endocarditis”. BMC Genomics 12.1 (2011).
This data publication is cited in the following publications:
This publication cites the following data publications:

11 Citations in Europe PMC

Data provided by Europe PubMed Central.

Comparative metagenomics of biogas-producing microbial communities from production-scale biogas plants operating under wet or dry fermentation conditions.
Stolze Y, Zakrzewski M, Maus I, Eikmeyer F, Jaenicke S, Rottmann N, Siebner C, Puhler A, Schluter A., Biotechnol Biofuels 8(), 2015
PMID: 25688290
Streptococcus gallolyticus subsp. gallolyticus from human and animal origins: genetic diversity, antimicrobial susceptibility, and characterization of a vancomycin-resistant calf isolate carrying a vanA-Tn1546-like element.
Romero-Hernandez B, Tedim AP, Sanchez-Herrero JF, Librado P, Rozas J, Munoz G, Baquero F, Canton R, Del Campo R., Antimicrob. Agents Chemother. 59(4), 2015
PMID: 25605355
Development and application of a multilocus sequence typing scheme for Streptococcus gallolyticus subsp. gallolyticus.
Dumke J, Hinse D, Vollmer T, Knabbe C, Dreier J., J. Clin. Microbiol. 52(7), 2014
PMID: 24789199
Comparative genomics of the dairy isolate Streptococcus macedonicus ACA-DC 198 against related members of the Streptococcus bovis/Streptococcus equinus complex.
Papadimitriou K, Anastasiou R, Mavrogonatou E, Blom J, Papandreou NC, Hamodrakas SJ, Ferreira S, Renault P, Supply P, Pot B, Tsakalidou E., BMC Genomics 15(), 2014
PMID: 24713045
Molecular identification key of the family Streptococcaceae.
Kanyo I, Nagy D., Acta Microbiol Immunol Hung 61(1), 2014
PMID: 24631752
Viridans and bovis group streptococci that cause infective endocarditis in two regions with contrasting epidemiology.
Naveen Kumar V, van der Linden M, Menon T, Nitsche-Schmitz DP., Int. J. Med. Microbiol. 304(3-4), 2014
PMID: 24220665
Molecular organization of small plasmids bearing blaTEM-1 and conferring resistance to β-lactams in Haemophilus influenzae.
Sondergaard A, San Millan A, Santos-Lopez A, Nielsen SM, Gonzalez-Zorn B, Norskov-Lauritsen N., Antimicrob. Agents Chemother. 56(9), 2012
PMID: 22733069
Characterization of a Streptococcus suis tet(O/W/32/O)-carrying element transferable to major streptococcal pathogens.
Palmieri C, Magi G, Mingoia M, Bagnarelli P, Ripa S, Varaldo PE, Facinelli B., Antimicrob. Agents Chemother. 56(9), 2012
PMID: 22710115

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 21824414
PubMed | Europe PMC

Search this title in

Google Scholar