Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons

Borriss R, Chen X-H, Rückert C, Blom J, Becker A, Baumgarth B, Fan B, Pukall R, Schumann P, Sproer C, Junge H, et al. (2011)
Int J Syst Evol Microbiol 61(8): 1786-1801.

Download
No fulltext has been uploaded. References only!
Journal Article | Original Article | Published | English

No fulltext has been uploaded

Author
; ; ; ; ; ; ; ; ; ; ;
All
Abstract
The whole-genome-sequenced rhizobacterium Bacillus amyloliquefaciens FZB42(T) (Chen et al., 2007) and other plant-associated strains of the genus Bacillus described as belonging to the species Bacillus amyloliquefaciens or Bacillus subtilis are used commercially to promote the growth and improve the health of crop plants. Previous investigations revealed that a group of strains represented a distinct ecotype related to B. amyloliquefaciens; however, the exact taxonomic position of this group remains elusive (Reva et al., 2004). In the present study, we demonstrated the ability of a group of Bacillus strains closely related to strain FZB42(T) to colonize Arabidopsis roots. On the basis of their phenotypic traits, the strains were similar to Bacillus amyloliquefaciens DSM 7(T) but differed considerably from this type strain in the DNA sequences of genes encoding 16S rRNA, gyrase subunit A (gyrA) and histidine kinase (cheA). Phylogenetic analysis performed with partial 16S rRNA, gyrA and cheA gene sequences revealed that the plant-associated strains of the genus Bacillus, including strain FZB42(T), formed a lineage, which could be distinguished from the cluster of strains closely related to B. amyloliquefaciens DSM 7(T). DNA-DNA hybridizations (DDH) performed with genomic DNA from strains DSM 7(T) and FZB42(T) yielded relatedness values of 63.7-71.2 %. Several methods of genomic analysis, such as direct whole-genome comparison, digital DDH and microarray-based comparative genomichybridization (M-CGH) were used as complementary tests. The group of plant-associated strains could be distinguished from strain DSM 7(T) and the type strain of B. subtilis by differences in the potential to synthesize non-ribosomal lipopeptides and polyketides. Based on the differences found in the marker gene sequences and the whole genomes of these strains, we propose two novel subspecies, designated B. amyloliquefaciens subsp. plantarum subsp. nov., with the type strain FZB42(T) ( = DSM 23117(T) = BGSC 10A6(T)), and B. amyloliquefaciens subsp. amyloliquefaciens subsp. nov., with the type strain DSM 7(T)( = ATCC 23350(T) = Fukumoto Strain F(T)), for plant-associated and non-plant-associated representatives, respecitvely. This is in agreement with results of DDH and M-CGH tests and the MALDI-TOF MS of cellular components, all of which suggested that the ecovars represent two different subspecies.
Publishing Year
ISSN
eISSN
PUB-ID

Cite this

Borriss R, Chen X-H, Rückert C, et al. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol. 2011;61(8):1786-1801.
Borriss, R., Chen, X. - H., Rückert, C., Blom, J., Becker, A., Baumgarth, B., Fan, B., et al. (2011). Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol, 61(8), 1786-1801. doi:10.1099/ijs.0.023267-0
Borriss, R., Chen, X. - H., Rückert, C., Blom, J., Becker, A., Baumgarth, B., Fan, B., Pukall, R., Schumann, P., Sproer, C., et al. (2011). Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol 61, 1786-1801.
Borriss, R., et al., 2011. Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol, 61(8), p 1786-1801.
R. Borriss, et al., “Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons”, Int J Syst Evol Microbiol, vol. 61, 2011, pp. 1786-1801.
Borriss, R., Chen, X.-H., Rückert, C., Blom, J., Becker, A., Baumgarth, B., Fan, B., Pukall, R., Schumann, P., Sproer, C., Junge, H., Vater, J., Pühler, A., Klenk, H.-P.: Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons. Int J Syst Evol Microbiol. 61, 1786-1801 (2011).
Borriss, Rainer, Chen, Xiao-Hua, Rückert, Christian, Blom, Jochen, Becker, Anke, Baumgarth, Birgit, Fan, Ben, Pukall, Rudiger, Schumann, Peter, Sproer, Cathrin, Junge, Helmut, Vater, Joachim, Pühler, Alfred, and Klenk, Hans-Peter. “Relationship of Bacillus amyloliquefaciens clades associated with strains DSM 7T and FZB42T: a proposal for Bacillus amyloliquefaciens subsp. amyloliquefaciens subsp. nov. and Bacillus amyloliquefaciens subsp. plantarum subsp. nov. based on complete genome sequence comparisons”. Int J Syst Evol Microbiol 61.8 (2011): 1786-1801.
This data publication is cited in the following publications:
This publication cites the following data publications:

63 Citations in Europe PMC

Data provided by Europe PubMed Central.

Bacillus amyloliquefaciens ssp. plantarum F11 isolated from Algerian salty lake as a source of biosurfactants and bioactive lipopeptides.
Daas MS, Acedo JZ, Rosana ARR, Orata FD, Reiz B, Zheng J, Nateche F, Case RJ, Kebbouche-Gana S, Vederas JC., FEMS Microbiol. Lett. 365(1), 2018
PMID: 29186395
Draft genome sequence of a thermostable, alkaliphilic α-amylase and protease producing Bacillus amyloliquefaciens strain KCP2.
Prajapati VS, Ray S, Narayan J, Joshi CC, Patel KC, Trivedi UB, Patel RM., 3 Biotech 7(6), 2017
PMID: 29071169
The contribution of genome mining strategies to the understanding of active principles of PGPR strains.
Paterson J, Jahanshah G, Li Y, Wang Q, Mehnaz S, Gross H., FEMS Microbiol. Ecol. 93(3), 2017
PMID: 27986826
New SigD-regulated genes identified in the rhizobacterium Bacillus amyloliquefaciens FZB42.
Fan B, Li YL, Mariappan A, Becker A, Wu XQ, Borriss R., Biol Open 5(12), 2016
PMID: 27797724
Whole genome shotgun sequence of Bacillus amyloliquefaciens TF28, a biocontrol entophytic bacterium.
Zhang S, Jiang W, Li J, Meng L, Cao X, Hu J, Liu Y, Chen J, Sha C., Stand Genomic Sci 11(), 2016
PMID: 27688836
Complete Genome Sequence of Bacillus methylotrophicus Strain B25, a Potential Plant Growth-Promoting Rhizobacterium.
Gerbore J, Brutel A, Lemainque A, Mairey B, Medigue C, Vallenet D, Lefort F, Grizard D., Genome Announc 4(2), 2016
PMID: 26966215
Complete genome sequence of the molybdenum-resistant bacterium Bacillus subtilis strain LM 4-2.
You XY, Wang H, Ren GY, Li JJ, Duan X, Zheng HJ, Jiang ZQ., Stand Genomic Sci 10(), 2015
PMID: 26664656
Single bacteria movement tracking by online microscopy--a proof of concept study.
Ziegler A, Schock-Kusch D, Bopp D, Dounia S, Radle M, Stahl U., PLoS ONE 10(4), 2015
PMID: 25849813
Draft Genome Sequence of Bacillus atrophaeus UCMB-5137, a Plant Growth-Promoting Rhizobacterium.
Chan WY, Dietel K, Lapa SV, Avdeeva LV, Borriss R, Reva ON., Genome Announc 1(3), 2013
PMID: 23788535
Does the applicability of Bacillus strains in probiotics rely upon their taxonomy?
Safronova LA, Zelena LB, Klochko VV, Reva ON., Can. J. Microbiol. 58(2), 2012
PMID: 22257263

Export

0 Marked Publications

Open Data PUB

Web of Science

View record in Web of Science®

Sources

PMID: 20817842
PubMed | Europe PMC

Search this title in

Google Scholar